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Identification of jasmonic acid-associated microRNAs and characterization of the regulatory roles of the miR319/TCP4 module under root-knot nematode stress in tomato.

Zhao W, Li Z, Fan J, Hu C, Yang R, Qi X, Chen H, Zhao F, Wang S - J. Exp. Bot. (2015)

Bottom Line: A total of 263 known miRNAs and 441 novel miRNAs were significantly regulated under RKN stress in the two libraries.Reverse genetics demonstrated that the miR319/TCP4 module affected JA synthetic genes and the endogenous JA level in leaves, thereby mediating RKN resistance.These results suggested that the action of miR319 in serving as a systemic signal responder and regulator that modulated the RKN systemic defensive response was mediated via JA.

View Article: PubMed Central - PubMed

Affiliation: Beijing Key Laboratory for Agricultural Application and New Technique, Plant Science and Technology College, Beijing University of Agriculture, Beijing, 102206, China.

No MeSH data available.


Related in: MedlinePlus

The length distribution and abundance of sRNAs in the libraries of WT (blank bars) and spr2 (grey bars).
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Figure 1: The length distribution and abundance of sRNAs in the libraries of WT (blank bars) and spr2 (grey bars).

Mentions: The 21 and 24 nucleotide RNAs were the most abundant sRNA species (Fig. 1). The sRNAs ranged from 21 to 24 nucleotides in length and comprised 81.46% of the WT and 76.94% of the spr2 transcripts, which was consistent with evidence found in the conifers Pinus contorta and Taxus chinensis, for which >50% of the sequenced sRNAs were 21 or 24 nucleotides (Qiu et al., 2009). In contrast to the results found in T. chinensis (Qiu et al., 2009), the percentage of 24 nucleotide sRNAs was much higher than the percentage of 21 nucleotide sRNAs in the WT in this study. The sRNAs had a strong bias for 21 nucleotide lengths in the spr2 strain, almost twice the rate in the WT strain. In addition, 24 nucleotide sRNAs dominated the WT strain, which was consistent with previous deep sequencing studies in which Hi-JA Arabidopsis exhibited a bias for 24 nucleotide sRNA populations (Zhang et al., 2012).


Identification of jasmonic acid-associated microRNAs and characterization of the regulatory roles of the miR319/TCP4 module under root-knot nematode stress in tomato.

Zhao W, Li Z, Fan J, Hu C, Yang R, Qi X, Chen H, Zhao F, Wang S - J. Exp. Bot. (2015)

The length distribution and abundance of sRNAs in the libraries of WT (blank bars) and spr2 (grey bars).
© Copyright Policy - creative-commons
Related In: Results  -  Collection

License 1 - License 2
Show All Figures
getmorefigures.php?uid=PMC4507771&req=5

Figure 1: The length distribution and abundance of sRNAs in the libraries of WT (blank bars) and spr2 (grey bars).
Mentions: The 21 and 24 nucleotide RNAs were the most abundant sRNA species (Fig. 1). The sRNAs ranged from 21 to 24 nucleotides in length and comprised 81.46% of the WT and 76.94% of the spr2 transcripts, which was consistent with evidence found in the conifers Pinus contorta and Taxus chinensis, for which >50% of the sequenced sRNAs were 21 or 24 nucleotides (Qiu et al., 2009). In contrast to the results found in T. chinensis (Qiu et al., 2009), the percentage of 24 nucleotide sRNAs was much higher than the percentage of 21 nucleotide sRNAs in the WT in this study. The sRNAs had a strong bias for 21 nucleotide lengths in the spr2 strain, almost twice the rate in the WT strain. In addition, 24 nucleotide sRNAs dominated the WT strain, which was consistent with previous deep sequencing studies in which Hi-JA Arabidopsis exhibited a bias for 24 nucleotide sRNA populations (Zhang et al., 2012).

Bottom Line: A total of 263 known miRNAs and 441 novel miRNAs were significantly regulated under RKN stress in the two libraries.Reverse genetics demonstrated that the miR319/TCP4 module affected JA synthetic genes and the endogenous JA level in leaves, thereby mediating RKN resistance.These results suggested that the action of miR319 in serving as a systemic signal responder and regulator that modulated the RKN systemic defensive response was mediated via JA.

View Article: PubMed Central - PubMed

Affiliation: Beijing Key Laboratory for Agricultural Application and New Technique, Plant Science and Technology College, Beijing University of Agriculture, Beijing, 102206, China.

No MeSH data available.


Related in: MedlinePlus