Limits...
Phosphorylation of TGB1 by protein kinase CK2 promotes barley stripe mosaic virus movement in monocots and dicots.

Hu Y, Li Z, Yuan C, Jin X, Yan L, Zhao X, Zhang Y, Jackson AO, Wang X, Han C, Yu J, Li D - J. Exp. Bot. (2015)

Bottom Line: Substitution of Thr-395 or Thr-401 with aspartic acid interfered with monocot and dicot cell-to-cell movement and the plants failed to develop systemic infections.The mutant XJTGB1T395A/T401A weakened in vitro interactions between XJTGB1 and XJTGB3 proteins but had little effect on XJTGB1 RNA-binding ability.Taken together, our results support a critical role of CK2 phosphorylation in the movement of BSMV in monocots and dicots, and provide new insights into the roles of phosphorylation in TGB protein functions.

View Article: PubMed Central - PubMed

Affiliation: State Key laboratory of Agro-Biotechnology and Ministry of Agriculture Key Laboratory of Soil Microbiology, College of Biological Sciences, China Agricultural University, Beijing 100193, PR China.

No MeSH data available.


Related in: MedlinePlus

Mutants affecting phosphorylation of the XJTGB1 protein have host-specific effects on systemic infectivity. (A) Symptoms of N. benthamiana elicited after infiltration with an Agrobacterium mixture harbouring pCa-αXJ, pCa-γXJ, and pCa-βXJ or its phosphorylation site mutants. Upper uninfiltrated leaf tissues were harvested and photographed at 10 dpi (top). CP ELISA (middle) and RNAγ RT-PCR amplification (bottom) were monitored to estimate the infectivity levels. (B, C) Systemic symptoms appearing in barley (B) and wheat (C) after inoculation with pT7-αXJ and pT7-γXJin vitro transcripts mixed with pT7-βXJ and various phosphorylation mutant transcripts. Leaves were photographed at 14 dpi (top) and all experiments were repeated three times. (This figure is available in colour at JXB online.)
© Copyright Policy - creative-commons
Related In: Results  -  Collection

License 1 - License 2
getmorefigures.php?uid=PMC4507770&req=5

Figure 5: Mutants affecting phosphorylation of the XJTGB1 protein have host-specific effects on systemic infectivity. (A) Symptoms of N. benthamiana elicited after infiltration with an Agrobacterium mixture harbouring pCa-αXJ, pCa-γXJ, and pCa-βXJ or its phosphorylation site mutants. Upper uninfiltrated leaf tissues were harvested and photographed at 10 dpi (top). CP ELISA (middle) and RNAγ RT-PCR amplification (bottom) were monitored to estimate the infectivity levels. (B, C) Systemic symptoms appearing in barley (B) and wheat (C) after inoculation with pT7-αXJ and pT7-γXJin vitro transcripts mixed with pT7-βXJ and various phosphorylation mutant transcripts. Leaves were photographed at 14 dpi (top) and all experiments were repeated three times. (This figure is available in colour at JXB online.)

Mentions: To determine whether the XJTGB1 mutants affected systemic movement in N. benthamiana, leaves were infiltrated with Agrobacterium harbouring the pCa-αXJ, wt pCa-βXJ, or individual TGB1 mutant derivatives, and the pCa-γXJ clones. Three independent experiments revealed that only wt XJTGB1 and the βXJ-TGB1T395A mutant, which exhibited slightly lower phosphorylation levels than the wt XJTGB1 protein, were able to establish systemic infections at 10 dpi (Fig. 5A, lanes 2 and 3). None of the remaining infiltrations containing single or double XJTGB1 mutants developed mosaic symptoms or invaded the upper uninoculated leaves as assessed by the absence of CP accumulation (Fig. 5A, lanes 4–11).


Phosphorylation of TGB1 by protein kinase CK2 promotes barley stripe mosaic virus movement in monocots and dicots.

Hu Y, Li Z, Yuan C, Jin X, Yan L, Zhao X, Zhang Y, Jackson AO, Wang X, Han C, Yu J, Li D - J. Exp. Bot. (2015)

Mutants affecting phosphorylation of the XJTGB1 protein have host-specific effects on systemic infectivity. (A) Symptoms of N. benthamiana elicited after infiltration with an Agrobacterium mixture harbouring pCa-αXJ, pCa-γXJ, and pCa-βXJ or its phosphorylation site mutants. Upper uninfiltrated leaf tissues were harvested and photographed at 10 dpi (top). CP ELISA (middle) and RNAγ RT-PCR amplification (bottom) were monitored to estimate the infectivity levels. (B, C) Systemic symptoms appearing in barley (B) and wheat (C) after inoculation with pT7-αXJ and pT7-γXJin vitro transcripts mixed with pT7-βXJ and various phosphorylation mutant transcripts. Leaves were photographed at 14 dpi (top) and all experiments were repeated three times. (This figure is available in colour at JXB online.)
© Copyright Policy - creative-commons
Related In: Results  -  Collection

License 1 - License 2
Show All Figures
getmorefigures.php?uid=PMC4507770&req=5

Figure 5: Mutants affecting phosphorylation of the XJTGB1 protein have host-specific effects on systemic infectivity. (A) Symptoms of N. benthamiana elicited after infiltration with an Agrobacterium mixture harbouring pCa-αXJ, pCa-γXJ, and pCa-βXJ or its phosphorylation site mutants. Upper uninfiltrated leaf tissues were harvested and photographed at 10 dpi (top). CP ELISA (middle) and RNAγ RT-PCR amplification (bottom) were monitored to estimate the infectivity levels. (B, C) Systemic symptoms appearing in barley (B) and wheat (C) after inoculation with pT7-αXJ and pT7-γXJin vitro transcripts mixed with pT7-βXJ and various phosphorylation mutant transcripts. Leaves were photographed at 14 dpi (top) and all experiments were repeated three times. (This figure is available in colour at JXB online.)
Mentions: To determine whether the XJTGB1 mutants affected systemic movement in N. benthamiana, leaves were infiltrated with Agrobacterium harbouring the pCa-αXJ, wt pCa-βXJ, or individual TGB1 mutant derivatives, and the pCa-γXJ clones. Three independent experiments revealed that only wt XJTGB1 and the βXJ-TGB1T395A mutant, which exhibited slightly lower phosphorylation levels than the wt XJTGB1 protein, were able to establish systemic infections at 10 dpi (Fig. 5A, lanes 2 and 3). None of the remaining infiltrations containing single or double XJTGB1 mutants developed mosaic symptoms or invaded the upper uninoculated leaves as assessed by the absence of CP accumulation (Fig. 5A, lanes 4–11).

Bottom Line: Substitution of Thr-395 or Thr-401 with aspartic acid interfered with monocot and dicot cell-to-cell movement and the plants failed to develop systemic infections.The mutant XJTGB1T395A/T401A weakened in vitro interactions between XJTGB1 and XJTGB3 proteins but had little effect on XJTGB1 RNA-binding ability.Taken together, our results support a critical role of CK2 phosphorylation in the movement of BSMV in monocots and dicots, and provide new insights into the roles of phosphorylation in TGB protein functions.

View Article: PubMed Central - PubMed

Affiliation: State Key laboratory of Agro-Biotechnology and Ministry of Agriculture Key Laboratory of Soil Microbiology, College of Biological Sciences, China Agricultural University, Beijing 100193, PR China.

No MeSH data available.


Related in: MedlinePlus