Limits...
Functional and expression analyses of kiwifruit SOC1-like genes suggest that they may not have a role in the transition to flowering but may affect the duration of dormancy.

Voogd C, Wang T, Varkonyi-Gasic E - J. Exp. Bot. (2015)

Bottom Line: Largely overlapping spatial domains but distinct expression profiles in buds were identified between the SOC1-like gene family members.Ectopic expression of AcSOC1e, AcSOC1i, and AcSOC1f in Actinidia chinensis had no impact on establishment of winter dormancy and failed to induce precocious flowering, but AcSOC1i reduced the duration of dormancy in the absence of winter chilling.These findings add to our understanding of the SOC1-like gene family and the potential diversification of SOC1 function in woody perennials.

View Article: PubMed Central - PubMed

Affiliation: The New Zealand Institute for Plant & Food Research Limited (Plant & Food Research), Mt Albert, Private Bag 92169, Auckland 1142, New Zealand.

No MeSH data available.


Related in: MedlinePlus

Constitutive expression of kiwifruit SOC1-like genes in transgenic kiwifruit Actinidia chinensis. (A) Relative expression of kiwifruit SOC1-like transgenes in A. chinensis transgenic lines. (B) Examples of A. chinensis single-node cuttings at the indicated stages. (C) Percentage of cuttings at the budbreak stage. (D) Average budbreak time of individual transgenic lines. Error bars represent SEs for three cuttings.
© Copyright Policy - creative-commons
Related In: Results  -  Collection

License 1 - License 2
getmorefigures.php?uid=PMC4507769&req=5

Figure 7: Constitutive expression of kiwifruit SOC1-like genes in transgenic kiwifruit Actinidia chinensis. (A) Relative expression of kiwifruit SOC1-like transgenes in A. chinensis transgenic lines. (B) Examples of A. chinensis single-node cuttings at the indicated stages. (C) Percentage of cuttings at the budbreak stage. (D) Average budbreak time of individual transgenic lines. Error bars represent SEs for three cuttings.

Mentions: To investigate the role of kiwifruit SOC1-like genes in kiwifruit, transgenic A. chinensis lines were generated using the AcSOC1e, AcSOC1f, and AcSOC1i coding sequence driven by the CaMV 35S promoter. These genes were chosen because of their similarity to Arabidopsis SOC1, high relative expression in buds (Fig. 3), and apparent sequential increase in transcription from late dormancy for AcSOC1e, pre-budbreak for AcSOC1i, and post-budbreak for AcSOC1f (Fig. 5). In addition, it was of interest to determine if kiwifruit SOC1-like genes affect the timing of the first flowering and reduce the juvenile stage. Most Actinidia species need several years to reach maturity and produce flowers and fruit. A minimum of eight independent lines were generated for each construct, with low to moderate transgene expression confirmed for 11 AcSOC1e lines and six of each of AcSOC1i and AcSOC1f lines (Fig. 7A; Supplementary Fig. S4 at JXB online). Normal regeneration, callus formation, and plantlet growth in tissue culture and in the soil were observed. The plants grown in the glasshouse in ambient conditions had the same appearance as controls and underwent normal cessation of active growth and leaf fall in autumn. Therefore, it is concluded that ectopic expression of AcSOC1e, AcSOC1i, and AcSOC1f has no impact on establishment of winter dormancy.


Functional and expression analyses of kiwifruit SOC1-like genes suggest that they may not have a role in the transition to flowering but may affect the duration of dormancy.

Voogd C, Wang T, Varkonyi-Gasic E - J. Exp. Bot. (2015)

Constitutive expression of kiwifruit SOC1-like genes in transgenic kiwifruit Actinidia chinensis. (A) Relative expression of kiwifruit SOC1-like transgenes in A. chinensis transgenic lines. (B) Examples of A. chinensis single-node cuttings at the indicated stages. (C) Percentage of cuttings at the budbreak stage. (D) Average budbreak time of individual transgenic lines. Error bars represent SEs for three cuttings.
© Copyright Policy - creative-commons
Related In: Results  -  Collection

License 1 - License 2
Show All Figures
getmorefigures.php?uid=PMC4507769&req=5

Figure 7: Constitutive expression of kiwifruit SOC1-like genes in transgenic kiwifruit Actinidia chinensis. (A) Relative expression of kiwifruit SOC1-like transgenes in A. chinensis transgenic lines. (B) Examples of A. chinensis single-node cuttings at the indicated stages. (C) Percentage of cuttings at the budbreak stage. (D) Average budbreak time of individual transgenic lines. Error bars represent SEs for three cuttings.
Mentions: To investigate the role of kiwifruit SOC1-like genes in kiwifruit, transgenic A. chinensis lines were generated using the AcSOC1e, AcSOC1f, and AcSOC1i coding sequence driven by the CaMV 35S promoter. These genes were chosen because of their similarity to Arabidopsis SOC1, high relative expression in buds (Fig. 3), and apparent sequential increase in transcription from late dormancy for AcSOC1e, pre-budbreak for AcSOC1i, and post-budbreak for AcSOC1f (Fig. 5). In addition, it was of interest to determine if kiwifruit SOC1-like genes affect the timing of the first flowering and reduce the juvenile stage. Most Actinidia species need several years to reach maturity and produce flowers and fruit. A minimum of eight independent lines were generated for each construct, with low to moderate transgene expression confirmed for 11 AcSOC1e lines and six of each of AcSOC1i and AcSOC1f lines (Fig. 7A; Supplementary Fig. S4 at JXB online). Normal regeneration, callus formation, and plantlet growth in tissue culture and in the soil were observed. The plants grown in the glasshouse in ambient conditions had the same appearance as controls and underwent normal cessation of active growth and leaf fall in autumn. Therefore, it is concluded that ectopic expression of AcSOC1e, AcSOC1i, and AcSOC1f has no impact on establishment of winter dormancy.

Bottom Line: Largely overlapping spatial domains but distinct expression profiles in buds were identified between the SOC1-like gene family members.Ectopic expression of AcSOC1e, AcSOC1i, and AcSOC1f in Actinidia chinensis had no impact on establishment of winter dormancy and failed to induce precocious flowering, but AcSOC1i reduced the duration of dormancy in the absence of winter chilling.These findings add to our understanding of the SOC1-like gene family and the potential diversification of SOC1 function in woody perennials.

View Article: PubMed Central - PubMed

Affiliation: The New Zealand Institute for Plant & Food Research Limited (Plant & Food Research), Mt Albert, Private Bag 92169, Auckland 1142, New Zealand.

No MeSH data available.


Related in: MedlinePlus