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Functional and expression analyses of kiwifruit SOC1-like genes suggest that they may not have a role in the transition to flowering but may affect the duration of dormancy.

Voogd C, Wang T, Varkonyi-Gasic E - J. Exp. Bot. (2015)

Bottom Line: Largely overlapping spatial domains but distinct expression profiles in buds were identified between the SOC1-like gene family members.Ectopic expression of AcSOC1e, AcSOC1i, and AcSOC1f in Actinidia chinensis had no impact on establishment of winter dormancy and failed to induce precocious flowering, but AcSOC1i reduced the duration of dormancy in the absence of winter chilling.These findings add to our understanding of the SOC1-like gene family and the potential diversification of SOC1 function in woody perennials.

View Article: PubMed Central - PubMed

Affiliation: The New Zealand Institute for Plant & Food Research Limited (Plant & Food Research), Mt Albert, Private Bag 92169, Auckland 1142, New Zealand.

No MeSH data available.


Related in: MedlinePlus

Summary of kiwifruit SOC1-like protein interactions detected by yeast two-hybrid analysis. (A) Homo- and heterodimerization of kiwifruit SOC1-like and Arabidopsis SOC1 proteins. (B, C) Heterodimerization of kiwifruit SOC1-like, SVP-like, and Arabidopsis SOC1, SVP, and AGL24. Black, very strong interaction; dark grey, strong interaction; light grey, weak interaction; white, no interaction. The summary is based on results presented in Supplementary Fig. S3 at JXB online.
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Figure 6: Summary of kiwifruit SOC1-like protein interactions detected by yeast two-hybrid analysis. (A) Homo- and heterodimerization of kiwifruit SOC1-like and Arabidopsis SOC1 proteins. (B, C) Heterodimerization of kiwifruit SOC1-like, SVP-like, and Arabidopsis SOC1, SVP, and AGL24. Black, very strong interaction; dark grey, strong interaction; light grey, weak interaction; white, no interaction. The summary is based on results presented in Supplementary Fig. S3 at JXB online.

Mentions: As an important floral integrator, Arabidopsis SOC1 has multiple interaction partners (Pelaz et al., 2001; de Folter et al., 2005). It was hypothesized that the kiwifruit SOC1-like proteins also exert their function through formation of homo- and heterodimers or higher order MADS box protein complexes. In particular, heterodimerization of SOC1 and AGL24 is essential for function (Lee et al., 2008), and the kiwifruit homologues of AGL24/ SVP genes are co-expressed with SOC1-like genes in vegetative plant tissues and show similar profiles of elevated expression over the bud dormancy period (Wu et al., 2012). A yeast two-hybrid screen was therefore performed to evaluate the interaction of the kiwifruit SOC1-like proteins with each other and with kiwifruit SVP proteins. Arabidopsis SOC1, AGL24, and SVP were included as controls. Only Arabidopsis SOC1, AcSOC1h, and, to a lesser extent, AcSOC1i were capable of homodimerization in this assay. AcSOC1h strongly interacted with the majority of other AcSOC1-like proteins, which were also mostly capable of interacting with SOC1; but other interactions were not detected (Fig. 6A; Supplementary Fig. S3 at JXB online). On the other hand, strong or very strong interactions were observed with SVP proteins, particularly kiwifruit SVP2 and SVP3 (Fig. 6B, C; Supplementary Fig. S3).


Functional and expression analyses of kiwifruit SOC1-like genes suggest that they may not have a role in the transition to flowering but may affect the duration of dormancy.

Voogd C, Wang T, Varkonyi-Gasic E - J. Exp. Bot. (2015)

Summary of kiwifruit SOC1-like protein interactions detected by yeast two-hybrid analysis. (A) Homo- and heterodimerization of kiwifruit SOC1-like and Arabidopsis SOC1 proteins. (B, C) Heterodimerization of kiwifruit SOC1-like, SVP-like, and Arabidopsis SOC1, SVP, and AGL24. Black, very strong interaction; dark grey, strong interaction; light grey, weak interaction; white, no interaction. The summary is based on results presented in Supplementary Fig. S3 at JXB online.
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Related In: Results  -  Collection

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Figure 6: Summary of kiwifruit SOC1-like protein interactions detected by yeast two-hybrid analysis. (A) Homo- and heterodimerization of kiwifruit SOC1-like and Arabidopsis SOC1 proteins. (B, C) Heterodimerization of kiwifruit SOC1-like, SVP-like, and Arabidopsis SOC1, SVP, and AGL24. Black, very strong interaction; dark grey, strong interaction; light grey, weak interaction; white, no interaction. The summary is based on results presented in Supplementary Fig. S3 at JXB online.
Mentions: As an important floral integrator, Arabidopsis SOC1 has multiple interaction partners (Pelaz et al., 2001; de Folter et al., 2005). It was hypothesized that the kiwifruit SOC1-like proteins also exert their function through formation of homo- and heterodimers or higher order MADS box protein complexes. In particular, heterodimerization of SOC1 and AGL24 is essential for function (Lee et al., 2008), and the kiwifruit homologues of AGL24/ SVP genes are co-expressed with SOC1-like genes in vegetative plant tissues and show similar profiles of elevated expression over the bud dormancy period (Wu et al., 2012). A yeast two-hybrid screen was therefore performed to evaluate the interaction of the kiwifruit SOC1-like proteins with each other and with kiwifruit SVP proteins. Arabidopsis SOC1, AGL24, and SVP were included as controls. Only Arabidopsis SOC1, AcSOC1h, and, to a lesser extent, AcSOC1i were capable of homodimerization in this assay. AcSOC1h strongly interacted with the majority of other AcSOC1-like proteins, which were also mostly capable of interacting with SOC1; but other interactions were not detected (Fig. 6A; Supplementary Fig. S3 at JXB online). On the other hand, strong or very strong interactions were observed with SVP proteins, particularly kiwifruit SVP2 and SVP3 (Fig. 6B, C; Supplementary Fig. S3).

Bottom Line: Largely overlapping spatial domains but distinct expression profiles in buds were identified between the SOC1-like gene family members.Ectopic expression of AcSOC1e, AcSOC1i, and AcSOC1f in Actinidia chinensis had no impact on establishment of winter dormancy and failed to induce precocious flowering, but AcSOC1i reduced the duration of dormancy in the absence of winter chilling.These findings add to our understanding of the SOC1-like gene family and the potential diversification of SOC1 function in woody perennials.

View Article: PubMed Central - PubMed

Affiliation: The New Zealand Institute for Plant & Food Research Limited (Plant & Food Research), Mt Albert, Private Bag 92169, Auckland 1142, New Zealand.

No MeSH data available.


Related in: MedlinePlus