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Functional and expression analyses of kiwifruit SOC1-like genes suggest that they may not have a role in the transition to flowering but may affect the duration of dormancy.

Voogd C, Wang T, Varkonyi-Gasic E - J. Exp. Bot. (2015)

Bottom Line: Largely overlapping spatial domains but distinct expression profiles in buds were identified between the SOC1-like gene family members.Ectopic expression of AcSOC1e, AcSOC1i, and AcSOC1f in Actinidia chinensis had no impact on establishment of winter dormancy and failed to induce precocious flowering, but AcSOC1i reduced the duration of dormancy in the absence of winter chilling.These findings add to our understanding of the SOC1-like gene family and the potential diversification of SOC1 function in woody perennials.

View Article: PubMed Central - PubMed

Affiliation: The New Zealand Institute for Plant & Food Research Limited (Plant & Food Research), Mt Albert, Private Bag 92169, Auckland 1142, New Zealand.

No MeSH data available.


Related in: MedlinePlus

Relative expression of kiwifruit SOC1-like genes in axillary buds during the growth and dormancy cycles, normalized to kiwifruit UBC9 and expressed as a ratio to the sample point with lowest expression, which was arbitrarily set to 1. Data points represent the mean ±SE for two replicates, the solid line represents samples collected from Hamilton, New Zealand, in 1995–1996, and the dotted line represents samples collected from Kerikeri, New Zealand, in 2008–2009. The vertical solid and dashed lines indicate budbreak time in Hamilton and Kerikeri, respectively. Hamilton is the region with colder winters (Supplementary Fig. S2 at JXB online).
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Figure 5: Relative expression of kiwifruit SOC1-like genes in axillary buds during the growth and dormancy cycles, normalized to kiwifruit UBC9 and expressed as a ratio to the sample point with lowest expression, which was arbitrarily set to 1. Data points represent the mean ±SE for two replicates, the solid line represents samples collected from Hamilton, New Zealand, in 1995–1996, and the dotted line represents samples collected from Kerikeri, New Zealand, in 2008–2009. The vertical solid and dashed lines indicate budbreak time in Hamilton and Kerikeri, respectively. Hamilton is the region with colder winters (Supplementary Fig. S2 at JXB online).

Mentions: Next, seasonal expression analysis was performed on lateral buds collected in regular intervals over the period of 1 year. Important developmental events, including floral evocation and bud dormancy, occur in kiwifruit buds over this period (Walton et al., 2001). The sampling was performed in two different years at different locations. Distinct expression profiles were obtained for the nine kiwifruit SOC1-like genes (Fig. 5). AcSOC1a, AcSOC1b, AcSOC1c, and AcSOC1d genes demonstrated increased expression throughout the winter dormancy period, particularly in samples collected from the region with colder winters (Supplementary Fig. S2 at JXB online). A similar, yet less strong pattern was found for AcSOC1e, AcSOC1g, and AcSOC1h, with AcSOC1e peaking later in winter and AcSOC1h increasing after winter in one location. AcSOC1i expression declined during early summer of the first season, remained low during early winter, then increased and peaked late in the winter, before budbreak. The level of transcripts of AcSOC1f slowly but consistently rose during the winter, and a sharp peak was detected after budbreak in one location.


Functional and expression analyses of kiwifruit SOC1-like genes suggest that they may not have a role in the transition to flowering but may affect the duration of dormancy.

Voogd C, Wang T, Varkonyi-Gasic E - J. Exp. Bot. (2015)

Relative expression of kiwifruit SOC1-like genes in axillary buds during the growth and dormancy cycles, normalized to kiwifruit UBC9 and expressed as a ratio to the sample point with lowest expression, which was arbitrarily set to 1. Data points represent the mean ±SE for two replicates, the solid line represents samples collected from Hamilton, New Zealand, in 1995–1996, and the dotted line represents samples collected from Kerikeri, New Zealand, in 2008–2009. The vertical solid and dashed lines indicate budbreak time in Hamilton and Kerikeri, respectively. Hamilton is the region with colder winters (Supplementary Fig. S2 at JXB online).
© Copyright Policy - creative-commons
Related In: Results  -  Collection

License 1 - License 2
Show All Figures
getmorefigures.php?uid=PMC4507769&req=5

Figure 5: Relative expression of kiwifruit SOC1-like genes in axillary buds during the growth and dormancy cycles, normalized to kiwifruit UBC9 and expressed as a ratio to the sample point with lowest expression, which was arbitrarily set to 1. Data points represent the mean ±SE for two replicates, the solid line represents samples collected from Hamilton, New Zealand, in 1995–1996, and the dotted line represents samples collected from Kerikeri, New Zealand, in 2008–2009. The vertical solid and dashed lines indicate budbreak time in Hamilton and Kerikeri, respectively. Hamilton is the region with colder winters (Supplementary Fig. S2 at JXB online).
Mentions: Next, seasonal expression analysis was performed on lateral buds collected in regular intervals over the period of 1 year. Important developmental events, including floral evocation and bud dormancy, occur in kiwifruit buds over this period (Walton et al., 2001). The sampling was performed in two different years at different locations. Distinct expression profiles were obtained for the nine kiwifruit SOC1-like genes (Fig. 5). AcSOC1a, AcSOC1b, AcSOC1c, and AcSOC1d genes demonstrated increased expression throughout the winter dormancy period, particularly in samples collected from the region with colder winters (Supplementary Fig. S2 at JXB online). A similar, yet less strong pattern was found for AcSOC1e, AcSOC1g, and AcSOC1h, with AcSOC1e peaking later in winter and AcSOC1h increasing after winter in one location. AcSOC1i expression declined during early summer of the first season, remained low during early winter, then increased and peaked late in the winter, before budbreak. The level of transcripts of AcSOC1f slowly but consistently rose during the winter, and a sharp peak was detected after budbreak in one location.

Bottom Line: Largely overlapping spatial domains but distinct expression profiles in buds were identified between the SOC1-like gene family members.Ectopic expression of AcSOC1e, AcSOC1i, and AcSOC1f in Actinidia chinensis had no impact on establishment of winter dormancy and failed to induce precocious flowering, but AcSOC1i reduced the duration of dormancy in the absence of winter chilling.These findings add to our understanding of the SOC1-like gene family and the potential diversification of SOC1 function in woody perennials.

View Article: PubMed Central - PubMed

Affiliation: The New Zealand Institute for Plant & Food Research Limited (Plant & Food Research), Mt Albert, Private Bag 92169, Auckland 1142, New Zealand.

No MeSH data available.


Related in: MedlinePlus