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The Arabidopsis thaliana elongator complex subunit 2 epigenetically affects root development.

Jia Y, Tian H, Li H, Yu Q, Wang L, Friml J, Ding Z - J. Exp. Bot. (2015)

Bottom Line: On the other hand, expression of the G2/M transition activator CYCB1 was substantially induced in elp2.The auxin efflux transporters PIN1 and PIN2 showed decreased protein levels and PIN1 also displayed mild polarity alterations in elp2, which resulted in a reduced auxin content in the root tip.Either the acetylation or methylation level of each of these genes differed between the mutant and the wild type, suggesting that the ELP2 regulation of root development involves the epigenetic modification of a range of transcription factors and other developmental regulators.

View Article: PubMed Central - PubMed

Affiliation: The Key Laboratory of Plant Cell Engineering and Germplasm Innovation, Ministry of Education, College of Life Science, Shandong University, Jinan 250100, China.

No MeSH data available.


Related in: MedlinePlus

DNA methylation level is altered in the elp2 mutant. (A) Placement of the primers is indicated. The rectangular box represents a CG island. (B–D) The methylation level of amplified fragments a (B), b (C) and c (D). The DNA was extracted from three biological replicates of both WT and elp2. Three replicates of 60 clones derived from each of WT and elp2 were bisulfite-sequenced to assess their methylation level. The data represent mean values with their associated SD (n=3); *, P<0.05, **, P<0.001.
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Figure 9: DNA methylation level is altered in the elp2 mutant. (A) Placement of the primers is indicated. The rectangular box represents a CG island. (B–D) The methylation level of amplified fragments a (B), b (C) and c (D). The DNA was extracted from three biological replicates of both WT and elp2. Three replicates of 60 clones derived from each of WT and elp2 were bisulfite-sequenced to assess their methylation level. The data represent mean values with their associated SD (n=3); *, P<0.05, **, P<0.001.

Mentions: ELP2 has recently been reported to be involved in somatic DNA demethylation/methylation, thus regulating pathogen-induced transcriptome reprogramming and plant immune responses (Wang et al., 2013). To address the reduced expression levels of transcription factors such as SCR and SHR, the increased expression of PID, and whether the increased expression of the cell cycle gene CYCB1 in elp2 are associated with the altered methylation levels, DNA methylation levels in CYCB1, PID, SHR and SCR were estimated by bisulfite sequencing. The level of methylation throughout both the promoter and coding regions of PID, SHR and SCR was low (Supplementary Figs S6, S7), indicating that these genes are not under the control of DNA demethylation/methylation. In the CYCB1 promoter, five cytosines showed a reduced frequency of methylation in the elp2 mutant compared to the WT. The mutant sequence was less methylated at the 3ʹ end of its coding region (Fig. 9). The observed reduced level of methylation in this gene was consistent with its up-regulation in the mutant.


The Arabidopsis thaliana elongator complex subunit 2 epigenetically affects root development.

Jia Y, Tian H, Li H, Yu Q, Wang L, Friml J, Ding Z - J. Exp. Bot. (2015)

DNA methylation level is altered in the elp2 mutant. (A) Placement of the primers is indicated. The rectangular box represents a CG island. (B–D) The methylation level of amplified fragments a (B), b (C) and c (D). The DNA was extracted from three biological replicates of both WT and elp2. Three replicates of 60 clones derived from each of WT and elp2 were bisulfite-sequenced to assess their methylation level. The data represent mean values with their associated SD (n=3); *, P<0.05, **, P<0.001.
© Copyright Policy - creative-commons
Related In: Results  -  Collection

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getmorefigures.php?uid=PMC4507768&req=5

Figure 9: DNA methylation level is altered in the elp2 mutant. (A) Placement of the primers is indicated. The rectangular box represents a CG island. (B–D) The methylation level of amplified fragments a (B), b (C) and c (D). The DNA was extracted from three biological replicates of both WT and elp2. Three replicates of 60 clones derived from each of WT and elp2 were bisulfite-sequenced to assess their methylation level. The data represent mean values with their associated SD (n=3); *, P<0.05, **, P<0.001.
Mentions: ELP2 has recently been reported to be involved in somatic DNA demethylation/methylation, thus regulating pathogen-induced transcriptome reprogramming and plant immune responses (Wang et al., 2013). To address the reduced expression levels of transcription factors such as SCR and SHR, the increased expression of PID, and whether the increased expression of the cell cycle gene CYCB1 in elp2 are associated with the altered methylation levels, DNA methylation levels in CYCB1, PID, SHR and SCR were estimated by bisulfite sequencing. The level of methylation throughout both the promoter and coding regions of PID, SHR and SCR was low (Supplementary Figs S6, S7), indicating that these genes are not under the control of DNA demethylation/methylation. In the CYCB1 promoter, five cytosines showed a reduced frequency of methylation in the elp2 mutant compared to the WT. The mutant sequence was less methylated at the 3ʹ end of its coding region (Fig. 9). The observed reduced level of methylation in this gene was consistent with its up-regulation in the mutant.

Bottom Line: On the other hand, expression of the G2/M transition activator CYCB1 was substantially induced in elp2.The auxin efflux transporters PIN1 and PIN2 showed decreased protein levels and PIN1 also displayed mild polarity alterations in elp2, which resulted in a reduced auxin content in the root tip.Either the acetylation or methylation level of each of these genes differed between the mutant and the wild type, suggesting that the ELP2 regulation of root development involves the epigenetic modification of a range of transcription factors and other developmental regulators.

View Article: PubMed Central - PubMed

Affiliation: The Key Laboratory of Plant Cell Engineering and Germplasm Innovation, Ministry of Education, College of Life Science, Shandong University, Jinan 250100, China.

No MeSH data available.


Related in: MedlinePlus