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The Arabidopsis thaliana elongator complex subunit 2 epigenetically affects root development.

Jia Y, Tian H, Li H, Yu Q, Wang L, Friml J, Ding Z - J. Exp. Bot. (2015)

Bottom Line: On the other hand, expression of the G2/M transition activator CYCB1 was substantially induced in elp2.The auxin efflux transporters PIN1 and PIN2 showed decreased protein levels and PIN1 also displayed mild polarity alterations in elp2, which resulted in a reduced auxin content in the root tip.Either the acetylation or methylation level of each of these genes differed between the mutant and the wild type, suggesting that the ELP2 regulation of root development involves the epigenetic modification of a range of transcription factors and other developmental regulators.

View Article: PubMed Central - PubMed

Affiliation: The Key Laboratory of Plant Cell Engineering and Germplasm Innovation, Ministry of Education, College of Life Science, Shandong University, Jinan 250100, China.

No MeSH data available.


Related in: MedlinePlus

Histone H3 acetylation levels in (A) PLT1, (B) PLT2, (C) SHR and (D) SCR. The placement of the primers is indicated. The relative amount of immunoprecipitated chromatin fragments in the elp2 mutant, as determined by qRT-PCR, was compared to that produced in the WT. The data represent mean values with their associated SD (n=3); *, P<0.05.
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Figure 8: Histone H3 acetylation levels in (A) PLT1, (B) PLT2, (C) SHR and (D) SCR. The placement of the primers is indicated. The relative amount of immunoprecipitated chromatin fragments in the elp2 mutant, as determined by qRT-PCR, was compared to that produced in the WT. The data represent mean values with their associated SD (n=3); *, P<0.05.

Mentions: The histone acetyltransferase of ELP3 has been confirmed both in yeast and plants (Winkler et al., 2002; Nelissen et al., 2010). To address whether the reduced expression level of several key transcription factors encoding genes such as PLT1, PLT2, SCR and SHR in elp2 may be associated with the reduced acetylation levels, we performed CHIP-PCR using an antibody specific for histone H3 acetylated at Lys-9 and -14 (H3K9/14ac). CHIP-PCR was performed to verify whether the reduced expression of PLT1, PLT2, SCR and SHR in the mutant was correlated with the altered acetylation level. The assay based on primers targeting the coding region and the 5ʹ-UTR of PLT1 and PLT2 showed that acetylation in this part of the gene was markedly reduced in the mutant (Fig. 8), while the acetylation level of the internal reference gene ACTIN2 was not changed (Supplementary Fig. S5A). A similar result was obtained with respect to the SHR and SCR coding regions which show reduced acetylation, but there was no difference between the WT and the mutant within promoter regions of both genes (Fig. 8). In addition, we also found a reduced acetylation level in 3ʹ coding region of PIN1, consistent with the reduced expression level of PIN1 (Supplementary Fig. S5B).


The Arabidopsis thaliana elongator complex subunit 2 epigenetically affects root development.

Jia Y, Tian H, Li H, Yu Q, Wang L, Friml J, Ding Z - J. Exp. Bot. (2015)

Histone H3 acetylation levels in (A) PLT1, (B) PLT2, (C) SHR and (D) SCR. The placement of the primers is indicated. The relative amount of immunoprecipitated chromatin fragments in the elp2 mutant, as determined by qRT-PCR, was compared to that produced in the WT. The data represent mean values with their associated SD (n=3); *, P<0.05.
© Copyright Policy - creative-commons
Related In: Results  -  Collection

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Figure 8: Histone H3 acetylation levels in (A) PLT1, (B) PLT2, (C) SHR and (D) SCR. The placement of the primers is indicated. The relative amount of immunoprecipitated chromatin fragments in the elp2 mutant, as determined by qRT-PCR, was compared to that produced in the WT. The data represent mean values with their associated SD (n=3); *, P<0.05.
Mentions: The histone acetyltransferase of ELP3 has been confirmed both in yeast and plants (Winkler et al., 2002; Nelissen et al., 2010). To address whether the reduced expression level of several key transcription factors encoding genes such as PLT1, PLT2, SCR and SHR in elp2 may be associated with the reduced acetylation levels, we performed CHIP-PCR using an antibody specific for histone H3 acetylated at Lys-9 and -14 (H3K9/14ac). CHIP-PCR was performed to verify whether the reduced expression of PLT1, PLT2, SCR and SHR in the mutant was correlated with the altered acetylation level. The assay based on primers targeting the coding region and the 5ʹ-UTR of PLT1 and PLT2 showed that acetylation in this part of the gene was markedly reduced in the mutant (Fig. 8), while the acetylation level of the internal reference gene ACTIN2 was not changed (Supplementary Fig. S5A). A similar result was obtained with respect to the SHR and SCR coding regions which show reduced acetylation, but there was no difference between the WT and the mutant within promoter regions of both genes (Fig. 8). In addition, we also found a reduced acetylation level in 3ʹ coding region of PIN1, consistent with the reduced expression level of PIN1 (Supplementary Fig. S5B).

Bottom Line: On the other hand, expression of the G2/M transition activator CYCB1 was substantially induced in elp2.The auxin efflux transporters PIN1 and PIN2 showed decreased protein levels and PIN1 also displayed mild polarity alterations in elp2, which resulted in a reduced auxin content in the root tip.Either the acetylation or methylation level of each of these genes differed between the mutant and the wild type, suggesting that the ELP2 regulation of root development involves the epigenetic modification of a range of transcription factors and other developmental regulators.

View Article: PubMed Central - PubMed

Affiliation: The Key Laboratory of Plant Cell Engineering and Germplasm Innovation, Ministry of Education, College of Life Science, Shandong University, Jinan 250100, China.

No MeSH data available.


Related in: MedlinePlus