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The Arabidopsis thaliana elongator complex subunit 2 epigenetically affects root development.

Jia Y, Tian H, Li H, Yu Q, Wang L, Friml J, Ding Z - J. Exp. Bot. (2015)

Bottom Line: On the other hand, expression of the G2/M transition activator CYCB1 was substantially induced in elp2.The auxin efflux transporters PIN1 and PIN2 showed decreased protein levels and PIN1 also displayed mild polarity alterations in elp2, which resulted in a reduced auxin content in the root tip.Either the acetylation or methylation level of each of these genes differed between the mutant and the wild type, suggesting that the ELP2 regulation of root development involves the epigenetic modification of a range of transcription factors and other developmental regulators.

View Article: PubMed Central - PubMed

Affiliation: The Key Laboratory of Plant Cell Engineering and Germplasm Innovation, Ministry of Education, College of Life Science, Shandong University, Jinan 250100, China.

No MeSH data available.


Related in: MedlinePlus

PIN1 and PIN2 localization and expression in the elp2 mutant. (A, B) PIN1 immunolocalization in five-day-old roots. (C, D) Expression and polarity of PIN1:PIN1-GFP in the WT and mutant root. (E, F) PIN2 immunolocalization in five-day old roots. (G, H) Expression and polarity of PIN2:PIN2-GFP in the WT and mutant root. (I) PID transcription assayed by qRT-PCR. The data represent mean values with their associated SD (n=3); **, P<0.001. Bars, 50 µm (A–H). (This figure is available in colour at JXB online.)
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Figure 7: PIN1 and PIN2 localization and expression in the elp2 mutant. (A, B) PIN1 immunolocalization in five-day-old roots. (C, D) Expression and polarity of PIN1:PIN1-GFP in the WT and mutant root. (E, F) PIN2 immunolocalization in five-day old roots. (G, H) Expression and polarity of PIN2:PIN2-GFP in the WT and mutant root. (I) PID transcription assayed by qRT-PCR. The data represent mean values with their associated SD (n=3); **, P<0.001. Bars, 50 µm (A–H). (This figure is available in colour at JXB online.)

Mentions: To address if the reduced auxin signalling in root tips of elp2 was a result of the affected polar auxin transport, we examined the polar localization of auxin efflux carriers such as PIN1 and PIN2 (Petrasek et al., 2006), which mediate auxin distribution in plants, including to the roots (Blilou et al., 2005; Wisniewska et al., 2006). Basal localized PIN1 in the stele and basal localized PIN2 in the cortex contribute to auxin transportation from shoot to root, thus contributing to maximum auxin formation in root tips and regulating root growth (Galweiler et al., 1998; Friml et al., 2003; Blilou et al., 2005; Wisniewska et al., 2006). However, in elp2, basal localization of PIN1 is less polar, and the increased lateral localization of PIN1 was observed from both our immunolocalization examinations with the anti-PIN1 antibody and confocal examinations with the PIN1-GFP line (Fig. 7A–D). Though apical PIN2 in the epidermis displays WT polarity in elp2 (Fig. 7E–H), both the protein expression levels of PIN1 and PIN2 were reduced in elp2 (Fig. 7C, D, G, H).


The Arabidopsis thaliana elongator complex subunit 2 epigenetically affects root development.

Jia Y, Tian H, Li H, Yu Q, Wang L, Friml J, Ding Z - J. Exp. Bot. (2015)

PIN1 and PIN2 localization and expression in the elp2 mutant. (A, B) PIN1 immunolocalization in five-day-old roots. (C, D) Expression and polarity of PIN1:PIN1-GFP in the WT and mutant root. (E, F) PIN2 immunolocalization in five-day old roots. (G, H) Expression and polarity of PIN2:PIN2-GFP in the WT and mutant root. (I) PID transcription assayed by qRT-PCR. The data represent mean values with their associated SD (n=3); **, P<0.001. Bars, 50 µm (A–H). (This figure is available in colour at JXB online.)
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Related In: Results  -  Collection

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Figure 7: PIN1 and PIN2 localization and expression in the elp2 mutant. (A, B) PIN1 immunolocalization in five-day-old roots. (C, D) Expression and polarity of PIN1:PIN1-GFP in the WT and mutant root. (E, F) PIN2 immunolocalization in five-day old roots. (G, H) Expression and polarity of PIN2:PIN2-GFP in the WT and mutant root. (I) PID transcription assayed by qRT-PCR. The data represent mean values with their associated SD (n=3); **, P<0.001. Bars, 50 µm (A–H). (This figure is available in colour at JXB online.)
Mentions: To address if the reduced auxin signalling in root tips of elp2 was a result of the affected polar auxin transport, we examined the polar localization of auxin efflux carriers such as PIN1 and PIN2 (Petrasek et al., 2006), which mediate auxin distribution in plants, including to the roots (Blilou et al., 2005; Wisniewska et al., 2006). Basal localized PIN1 in the stele and basal localized PIN2 in the cortex contribute to auxin transportation from shoot to root, thus contributing to maximum auxin formation in root tips and regulating root growth (Galweiler et al., 1998; Friml et al., 2003; Blilou et al., 2005; Wisniewska et al., 2006). However, in elp2, basal localization of PIN1 is less polar, and the increased lateral localization of PIN1 was observed from both our immunolocalization examinations with the anti-PIN1 antibody and confocal examinations with the PIN1-GFP line (Fig. 7A–D). Though apical PIN2 in the epidermis displays WT polarity in elp2 (Fig. 7E–H), both the protein expression levels of PIN1 and PIN2 were reduced in elp2 (Fig. 7C, D, G, H).

Bottom Line: On the other hand, expression of the G2/M transition activator CYCB1 was substantially induced in elp2.The auxin efflux transporters PIN1 and PIN2 showed decreased protein levels and PIN1 also displayed mild polarity alterations in elp2, which resulted in a reduced auxin content in the root tip.Either the acetylation or methylation level of each of these genes differed between the mutant and the wild type, suggesting that the ELP2 regulation of root development involves the epigenetic modification of a range of transcription factors and other developmental regulators.

View Article: PubMed Central - PubMed

Affiliation: The Key Laboratory of Plant Cell Engineering and Germplasm Innovation, Ministry of Education, College of Life Science, Shandong University, Jinan 250100, China.

No MeSH data available.


Related in: MedlinePlus