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The Arabidopsis thaliana elongator complex subunit 2 epigenetically affects root development.

Jia Y, Tian H, Li H, Yu Q, Wang L, Friml J, Ding Z - J. Exp. Bot. (2015)

Bottom Line: On the other hand, expression of the G2/M transition activator CYCB1 was substantially induced in elp2.The auxin efflux transporters PIN1 and PIN2 showed decreased protein levels and PIN1 also displayed mild polarity alterations in elp2, which resulted in a reduced auxin content in the root tip.Either the acetylation or methylation level of each of these genes differed between the mutant and the wild type, suggesting that the ELP2 regulation of root development involves the epigenetic modification of a range of transcription factors and other developmental regulators.

View Article: PubMed Central - PubMed

Affiliation: The Key Laboratory of Plant Cell Engineering and Germplasm Innovation, Ministry of Education, College of Life Science, Shandong University, Jinan 250100, China.

No MeSH data available.


Related in: MedlinePlus

The elp2 mutant exhibits reduced auxin contents in roots. (A, B) The mutant shows a reduced level of DR5rev:GFP expression in the root than the WT. (C, D) The mutant shows a reduced level of DR5:GUS expression in the root than the WT. (E) IAA content measurement of roots on 14-day-old seedlings of col and elp2. The data represent mean values of three independent biological repeats with their associated SD (n=3) *, P<0.05. Bars, 50 µm (A–D). (This figure is available in colour at JXB online.)
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Figure 6: The elp2 mutant exhibits reduced auxin contents in roots. (A, B) The mutant shows a reduced level of DR5rev:GFP expression in the root than the WT. (C, D) The mutant shows a reduced level of DR5:GUS expression in the root than the WT. (E) IAA content measurement of roots on 14-day-old seedlings of col and elp2. The data represent mean values of three independent biological repeats with their associated SD (n=3) *, P<0.05. Bars, 50 µm (A–D). (This figure is available in colour at JXB online.)

Mentions: Auxin gradients (Vanneste and Friml, 2009) are central to the robust development of the primary root in Arabidopsis, and the auxin gradient in the root tip could be instructive for the patterning of root distal stem cell (DSC) niches (Tian et al., 2013, 2014). To substantiate if the root defective phenotypes in elp2 are resulting from the changed auxin signalling in root tips, DR5rev:GFP and DR5:GUS synthetic auxin response reporters (Friml et al., 2003) were used to assay auxin signalling. The signal from both reporters was markedly lower in the mutant than in the WT root tip (Fig. 6A–D). The GUS enzyme activity assay using the MUG as the substrate further confirmed this difference (data not shown). Furthermore, we measured the free IAA content in roots of the elp2 mutant. Consistently, the elp2 mutant has reduced IAA content in roots compared to the wild-type control (Fig. 6E).


The Arabidopsis thaliana elongator complex subunit 2 epigenetically affects root development.

Jia Y, Tian H, Li H, Yu Q, Wang L, Friml J, Ding Z - J. Exp. Bot. (2015)

The elp2 mutant exhibits reduced auxin contents in roots. (A, B) The mutant shows a reduced level of DR5rev:GFP expression in the root than the WT. (C, D) The mutant shows a reduced level of DR5:GUS expression in the root than the WT. (E) IAA content measurement of roots on 14-day-old seedlings of col and elp2. The data represent mean values of three independent biological repeats with their associated SD (n=3) *, P<0.05. Bars, 50 µm (A–D). (This figure is available in colour at JXB online.)
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Related In: Results  -  Collection

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Figure 6: The elp2 mutant exhibits reduced auxin contents in roots. (A, B) The mutant shows a reduced level of DR5rev:GFP expression in the root than the WT. (C, D) The mutant shows a reduced level of DR5:GUS expression in the root than the WT. (E) IAA content measurement of roots on 14-day-old seedlings of col and elp2. The data represent mean values of three independent biological repeats with their associated SD (n=3) *, P<0.05. Bars, 50 µm (A–D). (This figure is available in colour at JXB online.)
Mentions: Auxin gradients (Vanneste and Friml, 2009) are central to the robust development of the primary root in Arabidopsis, and the auxin gradient in the root tip could be instructive for the patterning of root distal stem cell (DSC) niches (Tian et al., 2013, 2014). To substantiate if the root defective phenotypes in elp2 are resulting from the changed auxin signalling in root tips, DR5rev:GFP and DR5:GUS synthetic auxin response reporters (Friml et al., 2003) were used to assay auxin signalling. The signal from both reporters was markedly lower in the mutant than in the WT root tip (Fig. 6A–D). The GUS enzyme activity assay using the MUG as the substrate further confirmed this difference (data not shown). Furthermore, we measured the free IAA content in roots of the elp2 mutant. Consistently, the elp2 mutant has reduced IAA content in roots compared to the wild-type control (Fig. 6E).

Bottom Line: On the other hand, expression of the G2/M transition activator CYCB1 was substantially induced in elp2.The auxin efflux transporters PIN1 and PIN2 showed decreased protein levels and PIN1 also displayed mild polarity alterations in elp2, which resulted in a reduced auxin content in the root tip.Either the acetylation or methylation level of each of these genes differed between the mutant and the wild type, suggesting that the ELP2 regulation of root development involves the epigenetic modification of a range of transcription factors and other developmental regulators.

View Article: PubMed Central - PubMed

Affiliation: The Key Laboratory of Plant Cell Engineering and Germplasm Innovation, Ministry of Education, College of Life Science, Shandong University, Jinan 250100, China.

No MeSH data available.


Related in: MedlinePlus