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The Arabidopsis thaliana elongator complex subunit 2 epigenetically affects root development.

Jia Y, Tian H, Li H, Yu Q, Wang L, Friml J, Ding Z - J. Exp. Bot. (2015)

Bottom Line: On the other hand, expression of the G2/M transition activator CYCB1 was substantially induced in elp2.The auxin efflux transporters PIN1 and PIN2 showed decreased protein levels and PIN1 also displayed mild polarity alterations in elp2, which resulted in a reduced auxin content in the root tip.Either the acetylation or methylation level of each of these genes differed between the mutant and the wild type, suggesting that the ELP2 regulation of root development involves the epigenetic modification of a range of transcription factors and other developmental regulators.

View Article: PubMed Central - PubMed

Affiliation: The Key Laboratory of Plant Cell Engineering and Germplasm Innovation, Ministry of Education, College of Life Science, Shandong University, Jinan 250100, China.

No MeSH data available.


Related in: MedlinePlus

CYCB1 expression is increased in the elp2 mutant. (A, B) Expression of the CYCB1;1:GUS transgene, (C) transcription of CYCB1 assayed by qRT-PCR. The data represent mean values with their associated SD, (n=3); **, P<0.001. Bars, 50 µm (A, B). (This figure is available in colour at JXB online.)
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Figure 5: CYCB1 expression is increased in the elp2 mutant. (A, B) Expression of the CYCB1;1:GUS transgene, (C) transcription of CYCB1 assayed by qRT-PCR. The data represent mean values with their associated SD, (n=3); **, P<0.001. Bars, 50 µm (A, B). (This figure is available in colour at JXB online.)

Mentions: The increased QC division and reduced root meristem cell numbers in elp2 suggest that ELP2 is required for proper cell-cycle progression in the root. Cells at the G2-M phase were visualized by the transgene CYCB1;1:GUS which reflects the cell cycle activity, however timely degradation of proteins is necesssary to ensure the access of M phase (Colon-Carmona et al., 1999). In tobacco, the nondegradable Cyclin B1 results in abnormal cytokinesis and endomitosis (Weingartner et al., 2004). We observed that accumulation of the CYCB1;1:GUS signal in root meristem of elp2 was much stronger than that in the WT (Fig. 5A, B). In addition, stronger staining in the root QC of elp2 is consistent with the enhanced cell division in QC (Fig. 5A, B). Next, using qRT-PCR assays, we compared the CYCB1;1 expression level in the elp2 mutation and WT. In line with GUS staining, the elp2 mutant has high CYCB1;1 expression in the root (Fig. 5C). Therefore, our results showed that ELP2 is required for cell-cycle progression in the root.


The Arabidopsis thaliana elongator complex subunit 2 epigenetically affects root development.

Jia Y, Tian H, Li H, Yu Q, Wang L, Friml J, Ding Z - J. Exp. Bot. (2015)

CYCB1 expression is increased in the elp2 mutant. (A, B) Expression of the CYCB1;1:GUS transgene, (C) transcription of CYCB1 assayed by qRT-PCR. The data represent mean values with their associated SD, (n=3); **, P<0.001. Bars, 50 µm (A, B). (This figure is available in colour at JXB online.)
© Copyright Policy - creative-commons
Related In: Results  -  Collection

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Figure 5: CYCB1 expression is increased in the elp2 mutant. (A, B) Expression of the CYCB1;1:GUS transgene, (C) transcription of CYCB1 assayed by qRT-PCR. The data represent mean values with their associated SD, (n=3); **, P<0.001. Bars, 50 µm (A, B). (This figure is available in colour at JXB online.)
Mentions: The increased QC division and reduced root meristem cell numbers in elp2 suggest that ELP2 is required for proper cell-cycle progression in the root. Cells at the G2-M phase were visualized by the transgene CYCB1;1:GUS which reflects the cell cycle activity, however timely degradation of proteins is necesssary to ensure the access of M phase (Colon-Carmona et al., 1999). In tobacco, the nondegradable Cyclin B1 results in abnormal cytokinesis and endomitosis (Weingartner et al., 2004). We observed that accumulation of the CYCB1;1:GUS signal in root meristem of elp2 was much stronger than that in the WT (Fig. 5A, B). In addition, stronger staining in the root QC of elp2 is consistent with the enhanced cell division in QC (Fig. 5A, B). Next, using qRT-PCR assays, we compared the CYCB1;1 expression level in the elp2 mutation and WT. In line with GUS staining, the elp2 mutant has high CYCB1;1 expression in the root (Fig. 5C). Therefore, our results showed that ELP2 is required for cell-cycle progression in the root.

Bottom Line: On the other hand, expression of the G2/M transition activator CYCB1 was substantially induced in elp2.The auxin efflux transporters PIN1 and PIN2 showed decreased protein levels and PIN1 also displayed mild polarity alterations in elp2, which resulted in a reduced auxin content in the root tip.Either the acetylation or methylation level of each of these genes differed between the mutant and the wild type, suggesting that the ELP2 regulation of root development involves the epigenetic modification of a range of transcription factors and other developmental regulators.

View Article: PubMed Central - PubMed

Affiliation: The Key Laboratory of Plant Cell Engineering and Germplasm Innovation, Ministry of Education, College of Life Science, Shandong University, Jinan 250100, China.

No MeSH data available.


Related in: MedlinePlus