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The Arabidopsis thaliana elongator complex subunit 2 epigenetically affects root development.

Jia Y, Tian H, Li H, Yu Q, Wang L, Friml J, Ding Z - J. Exp. Bot. (2015)

Bottom Line: On the other hand, expression of the G2/M transition activator CYCB1 was substantially induced in elp2.The auxin efflux transporters PIN1 and PIN2 showed decreased protein levels and PIN1 also displayed mild polarity alterations in elp2, which resulted in a reduced auxin content in the root tip.Either the acetylation or methylation level of each of these genes differed between the mutant and the wild type, suggesting that the ELP2 regulation of root development involves the epigenetic modification of a range of transcription factors and other developmental regulators.

View Article: PubMed Central - PubMed

Affiliation: The Key Laboratory of Plant Cell Engineering and Germplasm Innovation, Ministry of Education, College of Life Science, Shandong University, Jinan 250100, China.

No MeSH data available.


Related in: MedlinePlus

SHR and SCR expression is reduced in the elp2 mutant. Expression of the transgenes (A, B) SHRpro:SHR-GFP, (C, D) SCRpro:SCR-GFP. qRT-PCR assay of the transcription of (E) SHR and (F) SCR. The data represent mean values with their associated SD (n=3); **, P<0.001. Bars, 50 µm (A–D). (This figure is available in colour at JXB online.)
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Figure 4: SHR and SCR expression is reduced in the elp2 mutant. Expression of the transgenes (A, B) SHRpro:SHR-GFP, (C, D) SCRpro:SCR-GFP. qRT-PCR assay of the transcription of (E) SHR and (F) SCR. The data represent mean values with their associated SD (n=3); **, P<0.001. Bars, 50 µm (A–D). (This figure is available in colour at JXB online.)

Mentions: To determine whether the effects of ELP2 on root stem cell niche maintenance is dependent on the well characterized root stem cell niche-defining transcription factors, we examined the expression of AP2 transcription factors such as PLT1 and PLT2 and GRAS transcription factors such as SCR and SHR (Helariutta et al., 2000; Sabatini et al., 2003; Aida et al., 2004; Galinha et al., 2007) in elp2. The expression of both PLT1pro:PLT1-YFP (Fig. 3C, D) and PLT2pro:PLT2-YFP (Fig. 3G, H) was strongly reduced in elp2 compared to the levels achieved in the WT, and the PLT1pro:CFP and PLT2pro:CFP behaved similarly (Fig. 3A, B, E, F). Consistently, a qRT-PCR assay showed that the expression level of PLT1 and PLT2 was lower in the elp2 mutant than in the WT (Fig. 3I, J). Thus the evidence was that ELP2 affected the transcription of both PLT1 and PLT2. The expression of SHR and SCR was investigated by comparing the expression of both SCRpro:SCR-GFP and SHRpro:SHR-GFP in both elp2 and WT. Both transgenes were strongly down-regulated in the mutant (Fig. 4A–D), a result that was confirmed by a qRT-PCR assay (Fig. 4E, F).


The Arabidopsis thaliana elongator complex subunit 2 epigenetically affects root development.

Jia Y, Tian H, Li H, Yu Q, Wang L, Friml J, Ding Z - J. Exp. Bot. (2015)

SHR and SCR expression is reduced in the elp2 mutant. Expression of the transgenes (A, B) SHRpro:SHR-GFP, (C, D) SCRpro:SCR-GFP. qRT-PCR assay of the transcription of (E) SHR and (F) SCR. The data represent mean values with their associated SD (n=3); **, P<0.001. Bars, 50 µm (A–D). (This figure is available in colour at JXB online.)
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Related In: Results  -  Collection

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Figure 4: SHR and SCR expression is reduced in the elp2 mutant. Expression of the transgenes (A, B) SHRpro:SHR-GFP, (C, D) SCRpro:SCR-GFP. qRT-PCR assay of the transcription of (E) SHR and (F) SCR. The data represent mean values with their associated SD (n=3); **, P<0.001. Bars, 50 µm (A–D). (This figure is available in colour at JXB online.)
Mentions: To determine whether the effects of ELP2 on root stem cell niche maintenance is dependent on the well characterized root stem cell niche-defining transcription factors, we examined the expression of AP2 transcription factors such as PLT1 and PLT2 and GRAS transcription factors such as SCR and SHR (Helariutta et al., 2000; Sabatini et al., 2003; Aida et al., 2004; Galinha et al., 2007) in elp2. The expression of both PLT1pro:PLT1-YFP (Fig. 3C, D) and PLT2pro:PLT2-YFP (Fig. 3G, H) was strongly reduced in elp2 compared to the levels achieved in the WT, and the PLT1pro:CFP and PLT2pro:CFP behaved similarly (Fig. 3A, B, E, F). Consistently, a qRT-PCR assay showed that the expression level of PLT1 and PLT2 was lower in the elp2 mutant than in the WT (Fig. 3I, J). Thus the evidence was that ELP2 affected the transcription of both PLT1 and PLT2. The expression of SHR and SCR was investigated by comparing the expression of both SCRpro:SCR-GFP and SHRpro:SHR-GFP in both elp2 and WT. Both transgenes were strongly down-regulated in the mutant (Fig. 4A–D), a result that was confirmed by a qRT-PCR assay (Fig. 4E, F).

Bottom Line: On the other hand, expression of the G2/M transition activator CYCB1 was substantially induced in elp2.The auxin efflux transporters PIN1 and PIN2 showed decreased protein levels and PIN1 also displayed mild polarity alterations in elp2, which resulted in a reduced auxin content in the root tip.Either the acetylation or methylation level of each of these genes differed between the mutant and the wild type, suggesting that the ELP2 regulation of root development involves the epigenetic modification of a range of transcription factors and other developmental regulators.

View Article: PubMed Central - PubMed

Affiliation: The Key Laboratory of Plant Cell Engineering and Germplasm Innovation, Ministry of Education, College of Life Science, Shandong University, Jinan 250100, China.

No MeSH data available.


Related in: MedlinePlus