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The Arabidopsis thaliana elongator complex subunit 2 epigenetically affects root development.

Jia Y, Tian H, Li H, Yu Q, Wang L, Friml J, Ding Z - J. Exp. Bot. (2015)

Bottom Line: On the other hand, expression of the G2/M transition activator CYCB1 was substantially induced in elp2.The auxin efflux transporters PIN1 and PIN2 showed decreased protein levels and PIN1 also displayed mild polarity alterations in elp2, which resulted in a reduced auxin content in the root tip.Either the acetylation or methylation level of each of these genes differed between the mutant and the wild type, suggesting that the ELP2 regulation of root development involves the epigenetic modification of a range of transcription factors and other developmental regulators.

View Article: PubMed Central - PubMed

Affiliation: The Key Laboratory of Plant Cell Engineering and Germplasm Innovation, Ministry of Education, College of Life Science, Shandong University, Jinan 250100, China.

No MeSH data available.


Related in: MedlinePlus

The elp2 mutant is defective with respect to root stem cell niche maintenance. Lugol stained (A) WT and (B) elp2 roots. Cell division in the mutant QC is enhanced (red arrowheads), while its distal stem cells are deficient in starch (yellow arrowheads). The root stem cell niche is shown boxed. Confocal micrographs of (C) WT and (D) elp2 plants expressing the transgene WOX5:GFP; the level of expression in the mutant is lower than in WT. Confocal micrographs illustrating the incorporation of EdU into (E) WT and (F) elp2 QC cells. The fluorescent signals show that the mutant QC is in a state of active division (white arrowheads). The QC is shown boxed. QC25 is expressed at a higher level in (G) the WT than in (H) the elp2 mutant. Bars: 20 µm (A, B, G, H); 10 µm (C–F). (This figure is available in colour at JXB online.)
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Figure 1: The elp2 mutant is defective with respect to root stem cell niche maintenance. Lugol stained (A) WT and (B) elp2 roots. Cell division in the mutant QC is enhanced (red arrowheads), while its distal stem cells are deficient in starch (yellow arrowheads). The root stem cell niche is shown boxed. Confocal micrographs of (C) WT and (D) elp2 plants expressing the transgene WOX5:GFP; the level of expression in the mutant is lower than in WT. Confocal micrographs illustrating the incorporation of EdU into (E) WT and (F) elp2 QC cells. The fluorescent signals show that the mutant QC is in a state of active division (white arrowheads). The QC is shown boxed. QC25 is expressed at a higher level in (G) the WT than in (H) the elp2 mutant. Bars: 20 µm (A, B, G, H); 10 µm (C–F). (This figure is available in colour at JXB online.)

Mentions: A search for T-DNA mutants displaying a defective root stem cell niche maintenance phenotype identified drs1, a mutant that exhibited enhanced root distal stem cell differentiation and increased mitotic activity in its QC (Fig. 1). When five-day-old seedlings were exposed for 24h to EdU [a thymidine analogue used to mark S-phase progression (Vanstraelen et al., 2009)], a stronger level of fluorescence was observed in the nuclei of mitotically active cells in the mutant than in WT, due to the coupling of EdU with Aexa Fluor 555. This assay indicated that mitotic activity was enhanced in elp2 QC cells (Fig. 1E, F). The QC specific transcription factor WOX5 was also strongly down-regulated (Fig. 1C, D), as was the signal produced by the QC marker QC25 (Fig. 1G, H).


The Arabidopsis thaliana elongator complex subunit 2 epigenetically affects root development.

Jia Y, Tian H, Li H, Yu Q, Wang L, Friml J, Ding Z - J. Exp. Bot. (2015)

The elp2 mutant is defective with respect to root stem cell niche maintenance. Lugol stained (A) WT and (B) elp2 roots. Cell division in the mutant QC is enhanced (red arrowheads), while its distal stem cells are deficient in starch (yellow arrowheads). The root stem cell niche is shown boxed. Confocal micrographs of (C) WT and (D) elp2 plants expressing the transgene WOX5:GFP; the level of expression in the mutant is lower than in WT. Confocal micrographs illustrating the incorporation of EdU into (E) WT and (F) elp2 QC cells. The fluorescent signals show that the mutant QC is in a state of active division (white arrowheads). The QC is shown boxed. QC25 is expressed at a higher level in (G) the WT than in (H) the elp2 mutant. Bars: 20 µm (A, B, G, H); 10 µm (C–F). (This figure is available in colour at JXB online.)
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Figure 1: The elp2 mutant is defective with respect to root stem cell niche maintenance. Lugol stained (A) WT and (B) elp2 roots. Cell division in the mutant QC is enhanced (red arrowheads), while its distal stem cells are deficient in starch (yellow arrowheads). The root stem cell niche is shown boxed. Confocal micrographs of (C) WT and (D) elp2 plants expressing the transgene WOX5:GFP; the level of expression in the mutant is lower than in WT. Confocal micrographs illustrating the incorporation of EdU into (E) WT and (F) elp2 QC cells. The fluorescent signals show that the mutant QC is in a state of active division (white arrowheads). The QC is shown boxed. QC25 is expressed at a higher level in (G) the WT than in (H) the elp2 mutant. Bars: 20 µm (A, B, G, H); 10 µm (C–F). (This figure is available in colour at JXB online.)
Mentions: A search for T-DNA mutants displaying a defective root stem cell niche maintenance phenotype identified drs1, a mutant that exhibited enhanced root distal stem cell differentiation and increased mitotic activity in its QC (Fig. 1). When five-day-old seedlings were exposed for 24h to EdU [a thymidine analogue used to mark S-phase progression (Vanstraelen et al., 2009)], a stronger level of fluorescence was observed in the nuclei of mitotically active cells in the mutant than in WT, due to the coupling of EdU with Aexa Fluor 555. This assay indicated that mitotic activity was enhanced in elp2 QC cells (Fig. 1E, F). The QC specific transcription factor WOX5 was also strongly down-regulated (Fig. 1C, D), as was the signal produced by the QC marker QC25 (Fig. 1G, H).

Bottom Line: On the other hand, expression of the G2/M transition activator CYCB1 was substantially induced in elp2.The auxin efflux transporters PIN1 and PIN2 showed decreased protein levels and PIN1 also displayed mild polarity alterations in elp2, which resulted in a reduced auxin content in the root tip.Either the acetylation or methylation level of each of these genes differed between the mutant and the wild type, suggesting that the ELP2 regulation of root development involves the epigenetic modification of a range of transcription factors and other developmental regulators.

View Article: PubMed Central - PubMed

Affiliation: The Key Laboratory of Plant Cell Engineering and Germplasm Innovation, Ministry of Education, College of Life Science, Shandong University, Jinan 250100, China.

No MeSH data available.


Related in: MedlinePlus