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Comparative proteomics of root plasma membrane proteins reveals the involvement of calcium signalling in NaCl-facilitated nitrate uptake in Salicornia europaea.

Nie L, Feng J, Fan P, Chen X, Guo J, Lv S, Bao H, Jia W, Tai F, Jiang P, Wang J, Li Y - J. Exp. Bot. (2015)

Bottom Line: The results showed that NaCl had a synergetic effect with nitrate on the growth of S. europaea.The application of the Ca(2+) channel blocker LaCl3 not only caused a decrease in nitrate uptake rate, but also attenuated the promoting effects of NaCl on nitrate uptake rates.Based on these results, a possible regulatory network of NaCl-facilitated nitrate uptake in S. europaea focusing on the involvement of Ca(2+) signalling was proposed.

View Article: PubMed Central - PubMed

Affiliation: Key Laboratory of Plant Molecular Physiology, Institute of Botany, Chinese Academy of Sciences, Beijing 100093, PR China.

No MeSH data available.


A putative regulatory network of NaCl-facilitated nitrate uptake in S. europaea. In S. europaea roots, the NaCl stimuli induced Ca2+ entry across the PM by activating ANN protein and some unknown Ca2+ channels, thereby causing [Ca2+]cyt elevation. CRT is involved in cytosolic Ca2+ homeostasis. The [Ca2+]cyt elevation is sensed by calcium signalling proteins including CaM/CML, CBL/CIPKs, and CDPKs/CPKs. 14-3-3 protein can be activated by CPK3 or some unknown Ca2+ signalling components. The calcium signalling proteins can transmit the signal into phosphorylation cascades capable of modulating gene expression and target protein activity, which may function in ion transport/homeostasis, membrane trafficking, redox homeostasis, and so on. CBL1/9 and CIPK8/23 are involved in the regulation of NRT1.1 by phosphorylation while 14-3-3 is involved in the regulation of NRT2s. NRs in the cytosol are targets of CPK17 and 14-3-3, which are also regulated by salt stress. The subsequent steps of N assimilation take place in plastids. GS participates in this process and can be regulated by CIPK and 14-3-3 through phosphorylation. The components in red indicate those identified in the present study.
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Figure 8: A putative regulatory network of NaCl-facilitated nitrate uptake in S. europaea. In S. europaea roots, the NaCl stimuli induced Ca2+ entry across the PM by activating ANN protein and some unknown Ca2+ channels, thereby causing [Ca2+]cyt elevation. CRT is involved in cytosolic Ca2+ homeostasis. The [Ca2+]cyt elevation is sensed by calcium signalling proteins including CaM/CML, CBL/CIPKs, and CDPKs/CPKs. 14-3-3 protein can be activated by CPK3 or some unknown Ca2+ signalling components. The calcium signalling proteins can transmit the signal into phosphorylation cascades capable of modulating gene expression and target protein activity, which may function in ion transport/homeostasis, membrane trafficking, redox homeostasis, and so on. CBL1/9 and CIPK8/23 are involved in the regulation of NRT1.1 by phosphorylation while 14-3-3 is involved in the regulation of NRT2s. NRs in the cytosol are targets of CPK17 and 14-3-3, which are also regulated by salt stress. The subsequent steps of N assimilation take place in plastids. GS participates in this process and can be regulated by CIPK and 14-3-3 through phosphorylation. The components in red indicate those identified in the present study.

Mentions: Based on the present results and previous studies, a possible regulatory network of NaCl-facilitated nitrate uptake in S. europaea focusing on the involvement of Ca2+ signalling is proposed (Fig. 8). In S. europaea roots, the NaCl stimuli induced Ca2+ entry across the PM by activating ANN protein and some unknown Ca2+ channels (Davies, 2014), thereby causing [Ca2+]cyt elevation. CRT is involved in cytosolic Ca2+ homeostasis (Jia et al., 2009). The [Ca2+]cyt elevation is sensed by calcium signalling proteins including CaM/CML, CBL/CIPKs, as well as CDPKs/CPKs (DeFalco et al., 2010). 14-3-3 protein can be activated by CPK3 or some unknown Ca2+ signalling components (Mehlmer et al., 2010). The calcium signalling proteins can transmit the signal into phosphorylation cascades capable of modulating gene expression, and target protein activity, which may function in ion transport/homeostasis, membrane trafficking, redox homeostasis, and so on (DeFalco et al., 2010). Thus, the elevated nitrate uptake rates and NR activity under NaCl treatment in S. europaea may be ascribed to calcium-dependent phosphorylation of proteins such as those described in the following sentences. CBL1/9 and CIPK8/23 are involved in the regulation of NRT1.1 by phosphorylation (Ho et al., 2009; Hu et al., 2009), while 14-3-3 is involved in the regulation of NRT2s (Guo et al., 2011). NRs in the cytosol are targets of CPK17 and 14-3-3, which are also regulated by salt stress (Lambeck et al., 2010). The subsequent steps of N assimilation in roots take place in plastids. GS participates in this processs and can be regulated by CIPK and 14-3-3 through phosphorylation (Masclaux-Daubresse et al., 2010).


Comparative proteomics of root plasma membrane proteins reveals the involvement of calcium signalling in NaCl-facilitated nitrate uptake in Salicornia europaea.

Nie L, Feng J, Fan P, Chen X, Guo J, Lv S, Bao H, Jia W, Tai F, Jiang P, Wang J, Li Y - J. Exp. Bot. (2015)

A putative regulatory network of NaCl-facilitated nitrate uptake in S. europaea. In S. europaea roots, the NaCl stimuli induced Ca2+ entry across the PM by activating ANN protein and some unknown Ca2+ channels, thereby causing [Ca2+]cyt elevation. CRT is involved in cytosolic Ca2+ homeostasis. The [Ca2+]cyt elevation is sensed by calcium signalling proteins including CaM/CML, CBL/CIPKs, and CDPKs/CPKs. 14-3-3 protein can be activated by CPK3 or some unknown Ca2+ signalling components. The calcium signalling proteins can transmit the signal into phosphorylation cascades capable of modulating gene expression and target protein activity, which may function in ion transport/homeostasis, membrane trafficking, redox homeostasis, and so on. CBL1/9 and CIPK8/23 are involved in the regulation of NRT1.1 by phosphorylation while 14-3-3 is involved in the regulation of NRT2s. NRs in the cytosol are targets of CPK17 and 14-3-3, which are also regulated by salt stress. The subsequent steps of N assimilation take place in plastids. GS participates in this process and can be regulated by CIPK and 14-3-3 through phosphorylation. The components in red indicate those identified in the present study.
© Copyright Policy - creative-commons
Related In: Results  -  Collection

License 1 - License 2
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Figure 8: A putative regulatory network of NaCl-facilitated nitrate uptake in S. europaea. In S. europaea roots, the NaCl stimuli induced Ca2+ entry across the PM by activating ANN protein and some unknown Ca2+ channels, thereby causing [Ca2+]cyt elevation. CRT is involved in cytosolic Ca2+ homeostasis. The [Ca2+]cyt elevation is sensed by calcium signalling proteins including CaM/CML, CBL/CIPKs, and CDPKs/CPKs. 14-3-3 protein can be activated by CPK3 or some unknown Ca2+ signalling components. The calcium signalling proteins can transmit the signal into phosphorylation cascades capable of modulating gene expression and target protein activity, which may function in ion transport/homeostasis, membrane trafficking, redox homeostasis, and so on. CBL1/9 and CIPK8/23 are involved in the regulation of NRT1.1 by phosphorylation while 14-3-3 is involved in the regulation of NRT2s. NRs in the cytosol are targets of CPK17 and 14-3-3, which are also regulated by salt stress. The subsequent steps of N assimilation take place in plastids. GS participates in this process and can be regulated by CIPK and 14-3-3 through phosphorylation. The components in red indicate those identified in the present study.
Mentions: Based on the present results and previous studies, a possible regulatory network of NaCl-facilitated nitrate uptake in S. europaea focusing on the involvement of Ca2+ signalling is proposed (Fig. 8). In S. europaea roots, the NaCl stimuli induced Ca2+ entry across the PM by activating ANN protein and some unknown Ca2+ channels (Davies, 2014), thereby causing [Ca2+]cyt elevation. CRT is involved in cytosolic Ca2+ homeostasis (Jia et al., 2009). The [Ca2+]cyt elevation is sensed by calcium signalling proteins including CaM/CML, CBL/CIPKs, as well as CDPKs/CPKs (DeFalco et al., 2010). 14-3-3 protein can be activated by CPK3 or some unknown Ca2+ signalling components (Mehlmer et al., 2010). The calcium signalling proteins can transmit the signal into phosphorylation cascades capable of modulating gene expression, and target protein activity, which may function in ion transport/homeostasis, membrane trafficking, redox homeostasis, and so on (DeFalco et al., 2010). Thus, the elevated nitrate uptake rates and NR activity under NaCl treatment in S. europaea may be ascribed to calcium-dependent phosphorylation of proteins such as those described in the following sentences. CBL1/9 and CIPK8/23 are involved in the regulation of NRT1.1 by phosphorylation (Ho et al., 2009; Hu et al., 2009), while 14-3-3 is involved in the regulation of NRT2s (Guo et al., 2011). NRs in the cytosol are targets of CPK17 and 14-3-3, which are also regulated by salt stress (Lambeck et al., 2010). The subsequent steps of N assimilation in roots take place in plastids. GS participates in this processs and can be regulated by CIPK and 14-3-3 through phosphorylation (Masclaux-Daubresse et al., 2010).

Bottom Line: The results showed that NaCl had a synergetic effect with nitrate on the growth of S. europaea.The application of the Ca(2+) channel blocker LaCl3 not only caused a decrease in nitrate uptake rate, but also attenuated the promoting effects of NaCl on nitrate uptake rates.Based on these results, a possible regulatory network of NaCl-facilitated nitrate uptake in S. europaea focusing on the involvement of Ca(2+) signalling was proposed.

View Article: PubMed Central - PubMed

Affiliation: Key Laboratory of Plant Molecular Physiology, Institute of Botany, Chinese Academy of Sciences, Beijing 100093, PR China.

No MeSH data available.