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Comparative proteomics of root plasma membrane proteins reveals the involvement of calcium signalling in NaCl-facilitated nitrate uptake in Salicornia europaea.

Nie L, Feng J, Fan P, Chen X, Guo J, Lv S, Bao H, Jia W, Tai F, Jiang P, Wang J, Li Y - J. Exp. Bot. (2015)

Bottom Line: The results showed that NaCl had a synergetic effect with nitrate on the growth of S. europaea.The application of the Ca(2+) channel blocker LaCl3 not only caused a decrease in nitrate uptake rate, but also attenuated the promoting effects of NaCl on nitrate uptake rates.Based on these results, a possible regulatory network of NaCl-facilitated nitrate uptake in S. europaea focusing on the involvement of Ca(2+) signalling was proposed.

View Article: PubMed Central - PubMed

Affiliation: Key Laboratory of Plant Molecular Physiology, Institute of Botany, Chinese Academy of Sciences, Beijing 100093, PR China.

No MeSH data available.


The involvement of Ca2+ signalling in NaCl-facilitated nitrate uptake in S. europaea. (A) The effects of NaCl on relative [Ca2+]cyt in S. europaea root tips. S. europaea seedlings treated with 200mM NaCl for 2h, 24h, 3 d, and 30 d were incubated with Fluo-4/AM, and root tips were observed by confocal microscopy (b, d, f, and h). The root tips without NaCl treatments served as controls (a, c, e, and g), a0 to h0 are light field images of the corresponding root tip. Scale bar=100 μm. (B) Quantification of average fluorescence intensities of root tips. Values are means ±SE (n=10). Asterisks indicate significant differences (P<0.05). (C) The effect of LaCl3 on nitrate uptake rates of S. europaea roots. LaCl3-pre-treated S. europaea seedlings were immersed in modified 1/2 Hoagland solutions with different NaCl and 15N-labelled KNO3 concentrations for 15min, and the content of 15N in roots was measured. Values are means ±SE (n=3). Different letters above the bars indicate significant differences at P<0.05, which were calculated separately under LN and HN conditions.
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Figure 7: The involvement of Ca2+ signalling in NaCl-facilitated nitrate uptake in S. europaea. (A) The effects of NaCl on relative [Ca2+]cyt in S. europaea root tips. S. europaea seedlings treated with 200mM NaCl for 2h, 24h, 3 d, and 30 d were incubated with Fluo-4/AM, and root tips were observed by confocal microscopy (b, d, f, and h). The root tips without NaCl treatments served as controls (a, c, e, and g), a0 to h0 are light field images of the corresponding root tip. Scale bar=100 μm. (B) Quantification of average fluorescence intensities of root tips. Values are means ±SE (n=10). Asterisks indicate significant differences (P<0.05). (C) The effect of LaCl3 on nitrate uptake rates of S. europaea roots. LaCl3-pre-treated S. europaea seedlings were immersed in modified 1/2 Hoagland solutions with different NaCl and 15N-labelled KNO3 concentrations for 15min, and the content of 15N in roots was measured. Values are means ±SE (n=3). Different letters above the bars indicate significant differences at P<0.05, which were calculated separately under LN and HN conditions.

Mentions: Previous studies have shown that [Ca2+]cyt fluctuations and the downstream calcium signalling proteins play pivotal roles in stress responses (Plieth, 2005). Thus, the dynamic changes of [Ca2+]cyt under 200mM NaCl treatment were determined in S. europaea roots using the Ca2+-sensitive fluorescent probe, Fluo-4/AM. In contrast to weak fluorescence detected in the control root tips, strong fluorescence was observed in roots treated with NaCl for 2h, 24h, and 3 d (Fig. 7A, B). Although the fluorescence from 30 d NaCl-treated root tips was weak, it was still significantly higher than that of the control. Statistical analysis of the mean intensity of fluorescence showed that [Ca2+]cyt was significantly elevated under NaCl treatments (Fig. 7B).


Comparative proteomics of root plasma membrane proteins reveals the involvement of calcium signalling in NaCl-facilitated nitrate uptake in Salicornia europaea.

Nie L, Feng J, Fan P, Chen X, Guo J, Lv S, Bao H, Jia W, Tai F, Jiang P, Wang J, Li Y - J. Exp. Bot. (2015)

The involvement of Ca2+ signalling in NaCl-facilitated nitrate uptake in S. europaea. (A) The effects of NaCl on relative [Ca2+]cyt in S. europaea root tips. S. europaea seedlings treated with 200mM NaCl for 2h, 24h, 3 d, and 30 d were incubated with Fluo-4/AM, and root tips were observed by confocal microscopy (b, d, f, and h). The root tips without NaCl treatments served as controls (a, c, e, and g), a0 to h0 are light field images of the corresponding root tip. Scale bar=100 μm. (B) Quantification of average fluorescence intensities of root tips. Values are means ±SE (n=10). Asterisks indicate significant differences (P<0.05). (C) The effect of LaCl3 on nitrate uptake rates of S. europaea roots. LaCl3-pre-treated S. europaea seedlings were immersed in modified 1/2 Hoagland solutions with different NaCl and 15N-labelled KNO3 concentrations for 15min, and the content of 15N in roots was measured. Values are means ±SE (n=3). Different letters above the bars indicate significant differences at P<0.05, which were calculated separately under LN and HN conditions.
© Copyright Policy - creative-commons
Related In: Results  -  Collection

License 1 - License 2
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Figure 7: The involvement of Ca2+ signalling in NaCl-facilitated nitrate uptake in S. europaea. (A) The effects of NaCl on relative [Ca2+]cyt in S. europaea root tips. S. europaea seedlings treated with 200mM NaCl for 2h, 24h, 3 d, and 30 d were incubated with Fluo-4/AM, and root tips were observed by confocal microscopy (b, d, f, and h). The root tips without NaCl treatments served as controls (a, c, e, and g), a0 to h0 are light field images of the corresponding root tip. Scale bar=100 μm. (B) Quantification of average fluorescence intensities of root tips. Values are means ±SE (n=10). Asterisks indicate significant differences (P<0.05). (C) The effect of LaCl3 on nitrate uptake rates of S. europaea roots. LaCl3-pre-treated S. europaea seedlings were immersed in modified 1/2 Hoagland solutions with different NaCl and 15N-labelled KNO3 concentrations for 15min, and the content of 15N in roots was measured. Values are means ±SE (n=3). Different letters above the bars indicate significant differences at P<0.05, which were calculated separately under LN and HN conditions.
Mentions: Previous studies have shown that [Ca2+]cyt fluctuations and the downstream calcium signalling proteins play pivotal roles in stress responses (Plieth, 2005). Thus, the dynamic changes of [Ca2+]cyt under 200mM NaCl treatment were determined in S. europaea roots using the Ca2+-sensitive fluorescent probe, Fluo-4/AM. In contrast to weak fluorescence detected in the control root tips, strong fluorescence was observed in roots treated with NaCl for 2h, 24h, and 3 d (Fig. 7A, B). Although the fluorescence from 30 d NaCl-treated root tips was weak, it was still significantly higher than that of the control. Statistical analysis of the mean intensity of fluorescence showed that [Ca2+]cyt was significantly elevated under NaCl treatments (Fig. 7B).

Bottom Line: The results showed that NaCl had a synergetic effect with nitrate on the growth of S. europaea.The application of the Ca(2+) channel blocker LaCl3 not only caused a decrease in nitrate uptake rate, but also attenuated the promoting effects of NaCl on nitrate uptake rates.Based on these results, a possible regulatory network of NaCl-facilitated nitrate uptake in S. europaea focusing on the involvement of Ca(2+) signalling was proposed.

View Article: PubMed Central - PubMed

Affiliation: Key Laboratory of Plant Molecular Physiology, Institute of Botany, Chinese Academy of Sciences, Beijing 100093, PR China.

No MeSH data available.