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Comparative proteomics of root plasma membrane proteins reveals the involvement of calcium signalling in NaCl-facilitated nitrate uptake in Salicornia europaea.

Nie L, Feng J, Fan P, Chen X, Guo J, Lv S, Bao H, Jia W, Tai F, Jiang P, Wang J, Li Y - J. Exp. Bot. (2015)

Bottom Line: The results showed that NaCl had a synergetic effect with nitrate on the growth of S. europaea.The application of the Ca(2+) channel blocker LaCl3 not only caused a decrease in nitrate uptake rate, but also attenuated the promoting effects of NaCl on nitrate uptake rates.Based on these results, a possible regulatory network of NaCl-facilitated nitrate uptake in S. europaea focusing on the involvement of Ca(2+) signalling was proposed.

View Article: PubMed Central - PubMed

Affiliation: Key Laboratory of Plant Molecular Physiology, Institute of Botany, Chinese Academy of Sciences, Beijing 100093, PR China.

No MeSH data available.


Related in: MedlinePlus

Expression profiles of calcium signalling components at protein and mRNA levels in response to NaCl and nitrate. The images on the left are magnified views of 2D-DIGE gel sections containing the spot of the calcium signalling components. The corresponding expression files at protein and mRNA levels are listed on the right. Expression levels of mRNAs were evaluated using qRT-PCR, n=3. The protein expression values were generated using (RV¯treatments/RV¯LN). RV¯, means of normal volume/normal standard volume, n=5. Values are means ±SD.
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Figure 6: Expression profiles of calcium signalling components at protein and mRNA levels in response to NaCl and nitrate. The images on the left are magnified views of 2D-DIGE gel sections containing the spot of the calcium signalling components. The corresponding expression files at protein and mRNA levels are listed on the right. Expression levels of mRNAs were evaluated using qRT-PCR, n=3. The protein expression values were generated using (RV¯treatments/RV¯LN). RV¯, means of normal volume/normal standard volume, n=5. Values are means ±SD.

Mentions: Eight calcium signalling components were identified by 2D-DIGE, and seven of these components were up-regulated, whereas the other one was down-regulated by NaCl treatment under HN and/or LN conditions (Fig. 6). qRT-PCR was performed to examine whether the eight components were also regulated at the transcription level. As shown in Fig. 6, the mRNA transcripts of the eight proteins were all up-regulated by NaCl treatment under HN and/or LN conditions. Two of them, annexin (ANN)-like protein RJ4-like and PBI41 14-3-3, displayed consistency between mRNA and protein variation trends, whereas the other six proteins showed some discrepancies (Fig. 6).


Comparative proteomics of root plasma membrane proteins reveals the involvement of calcium signalling in NaCl-facilitated nitrate uptake in Salicornia europaea.

Nie L, Feng J, Fan P, Chen X, Guo J, Lv S, Bao H, Jia W, Tai F, Jiang P, Wang J, Li Y - J. Exp. Bot. (2015)

Expression profiles of calcium signalling components at protein and mRNA levels in response to NaCl and nitrate. The images on the left are magnified views of 2D-DIGE gel sections containing the spot of the calcium signalling components. The corresponding expression files at protein and mRNA levels are listed on the right. Expression levels of mRNAs were evaluated using qRT-PCR, n=3. The protein expression values were generated using (RV¯treatments/RV¯LN). RV¯, means of normal volume/normal standard volume, n=5. Values are means ±SD.
© Copyright Policy - creative-commons
Related In: Results  -  Collection

License 1 - License 2
Show All Figures
getmorefigures.php?uid=PMC4507759&req=5

Figure 6: Expression profiles of calcium signalling components at protein and mRNA levels in response to NaCl and nitrate. The images on the left are magnified views of 2D-DIGE gel sections containing the spot of the calcium signalling components. The corresponding expression files at protein and mRNA levels are listed on the right. Expression levels of mRNAs were evaluated using qRT-PCR, n=3. The protein expression values were generated using (RV¯treatments/RV¯LN). RV¯, means of normal volume/normal standard volume, n=5. Values are means ±SD.
Mentions: Eight calcium signalling components were identified by 2D-DIGE, and seven of these components were up-regulated, whereas the other one was down-regulated by NaCl treatment under HN and/or LN conditions (Fig. 6). qRT-PCR was performed to examine whether the eight components were also regulated at the transcription level. As shown in Fig. 6, the mRNA transcripts of the eight proteins were all up-regulated by NaCl treatment under HN and/or LN conditions. Two of them, annexin (ANN)-like protein RJ4-like and PBI41 14-3-3, displayed consistency between mRNA and protein variation trends, whereas the other six proteins showed some discrepancies (Fig. 6).

Bottom Line: The results showed that NaCl had a synergetic effect with nitrate on the growth of S. europaea.The application of the Ca(2+) channel blocker LaCl3 not only caused a decrease in nitrate uptake rate, but also attenuated the promoting effects of NaCl on nitrate uptake rates.Based on these results, a possible regulatory network of NaCl-facilitated nitrate uptake in S. europaea focusing on the involvement of Ca(2+) signalling was proposed.

View Article: PubMed Central - PubMed

Affiliation: Key Laboratory of Plant Molecular Physiology, Institute of Botany, Chinese Academy of Sciences, Beijing 100093, PR China.

No MeSH data available.


Related in: MedlinePlus