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Comparative proteomics of root plasma membrane proteins reveals the involvement of calcium signalling in NaCl-facilitated nitrate uptake in Salicornia europaea.

Nie L, Feng J, Fan P, Chen X, Guo J, Lv S, Bao H, Jia W, Tai F, Jiang P, Wang J, Li Y - J. Exp. Bot. (2015)

Bottom Line: The results showed that NaCl had a synergetic effect with nitrate on the growth of S. europaea.The application of the Ca(2+) channel blocker LaCl3 not only caused a decrease in nitrate uptake rate, but also attenuated the promoting effects of NaCl on nitrate uptake rates.Based on these results, a possible regulatory network of NaCl-facilitated nitrate uptake in S. europaea focusing on the involvement of Ca(2+) signalling was proposed.

View Article: PubMed Central - PubMed

Affiliation: Key Laboratory of Plant Molecular Physiology, Institute of Botany, Chinese Academy of Sciences, Beijing 100093, PR China.

No MeSH data available.


Related in: MedlinePlus

Clustering analysis of differentially accumulated proteins. The 81 differentially accumulated proteins were grouped into eight clusters according to their change patterns, which are illustrated by reaction norm diagrams consisting of a graphical representation highlighting the contribution of NaCl treatment to the observed protein abundance under nitrate treatment. Each row in the heat map indicates a single protein, whose expression values were generated using Log2 (RV¯treatments/RV¯LN). For each protein, the spot number and the protein name with its ANOVA effect (S, N, and/or S×N) are shown. Reaction norms were generated using the means of Log2 (RV¯treatments/RV¯LN) of spots for each defined cluster. RV¯, means of normal volume/normal standard volume.
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Figure 5: Clustering analysis of differentially accumulated proteins. The 81 differentially accumulated proteins were grouped into eight clusters according to their change patterns, which are illustrated by reaction norm diagrams consisting of a graphical representation highlighting the contribution of NaCl treatment to the observed protein abundance under nitrate treatment. Each row in the heat map indicates a single protein, whose expression values were generated using Log2 (RV¯treatments/RV¯LN). For each protein, the spot number and the protein name with its ANOVA effect (S, N, and/or S×N) are shown. Reaction norms were generated using the means of Log2 (RV¯treatments/RV¯LN) of spots for each defined cluster. RV¯, means of normal volume/normal standard volume.

Mentions: The 81 annotated, differentially accumulated proteins were further grouped into eight distinct clusters (Clusters I–VIII, Fig. 5; Supplementary Table S4 at JXB online) according to their change in abundance pattern. These clusters were illustrated by reaction norm diagrams consisting of a graphical representation highlighting the contribution of NaCl treatment (0mM and 200mM) to the observed protein abundance for different levels of nitrate treatments (0.1mM and 10mM). Cluster I contained 16 proteins whose accumulation increased significantly by NaCl treatment under both LN and HN conditions. Seven of these proteins were also significantly changed under nitrate treatments. Cluster II comprised nine proteins that were significantly repressed by the NaCl treatment under both LN and HN conditions. Four of these nine proteins changed significantly under nitrate treatments. Clusters III and IV comprised proteins whose abundance was increased or decreased significantly by NaCl treatment, but only under HN conditions. Cluster III included 10 proteins, three of which were significantly changed under nitrate treatments. Cluster IV contained six proteins, all of which were significantly changed under nitrate treatments. The largest cluster (Cluster V) comprised 19 proteins, which showed increased accumulation by NaCl treatment under LN conditions. Sixteen of these 19 proteins were also significantly changed under nitrate treatments. Only one protein in Cluster VI was repressed by NaCl treatment under LN conditions, and this protein was also down-regulated under nitrate supplement without NaCl. Cluster VII comprised seven proteins showing opposite responses to NaCl under LN and HN conditions. All of these proteins displayed a clear S×N interaction effect. Cluster VIII comprised 13 proteins whose abundance was not changed significantly by NaCl treatment whether under LN or HN conditions, but the abundance of such proteins changed significantly in response to nitrate.


Comparative proteomics of root plasma membrane proteins reveals the involvement of calcium signalling in NaCl-facilitated nitrate uptake in Salicornia europaea.

Nie L, Feng J, Fan P, Chen X, Guo J, Lv S, Bao H, Jia W, Tai F, Jiang P, Wang J, Li Y - J. Exp. Bot. (2015)

Clustering analysis of differentially accumulated proteins. The 81 differentially accumulated proteins were grouped into eight clusters according to their change patterns, which are illustrated by reaction norm diagrams consisting of a graphical representation highlighting the contribution of NaCl treatment to the observed protein abundance under nitrate treatment. Each row in the heat map indicates a single protein, whose expression values were generated using Log2 (RV¯treatments/RV¯LN). For each protein, the spot number and the protein name with its ANOVA effect (S, N, and/or S×N) are shown. Reaction norms were generated using the means of Log2 (RV¯treatments/RV¯LN) of spots for each defined cluster. RV¯, means of normal volume/normal standard volume.
© Copyright Policy - creative-commons
Related In: Results  -  Collection

License 1 - License 2
Show All Figures
getmorefigures.php?uid=PMC4507759&req=5

Figure 5: Clustering analysis of differentially accumulated proteins. The 81 differentially accumulated proteins were grouped into eight clusters according to their change patterns, which are illustrated by reaction norm diagrams consisting of a graphical representation highlighting the contribution of NaCl treatment to the observed protein abundance under nitrate treatment. Each row in the heat map indicates a single protein, whose expression values were generated using Log2 (RV¯treatments/RV¯LN). For each protein, the spot number and the protein name with its ANOVA effect (S, N, and/or S×N) are shown. Reaction norms were generated using the means of Log2 (RV¯treatments/RV¯LN) of spots for each defined cluster. RV¯, means of normal volume/normal standard volume.
Mentions: The 81 annotated, differentially accumulated proteins were further grouped into eight distinct clusters (Clusters I–VIII, Fig. 5; Supplementary Table S4 at JXB online) according to their change in abundance pattern. These clusters were illustrated by reaction norm diagrams consisting of a graphical representation highlighting the contribution of NaCl treatment (0mM and 200mM) to the observed protein abundance for different levels of nitrate treatments (0.1mM and 10mM). Cluster I contained 16 proteins whose accumulation increased significantly by NaCl treatment under both LN and HN conditions. Seven of these proteins were also significantly changed under nitrate treatments. Cluster II comprised nine proteins that were significantly repressed by the NaCl treatment under both LN and HN conditions. Four of these nine proteins changed significantly under nitrate treatments. Clusters III and IV comprised proteins whose abundance was increased or decreased significantly by NaCl treatment, but only under HN conditions. Cluster III included 10 proteins, three of which were significantly changed under nitrate treatments. Cluster IV contained six proteins, all of which were significantly changed under nitrate treatments. The largest cluster (Cluster V) comprised 19 proteins, which showed increased accumulation by NaCl treatment under LN conditions. Sixteen of these 19 proteins were also significantly changed under nitrate treatments. Only one protein in Cluster VI was repressed by NaCl treatment under LN conditions, and this protein was also down-regulated under nitrate supplement without NaCl. Cluster VII comprised seven proteins showing opposite responses to NaCl under LN and HN conditions. All of these proteins displayed a clear S×N interaction effect. Cluster VIII comprised 13 proteins whose abundance was not changed significantly by NaCl treatment whether under LN or HN conditions, but the abundance of such proteins changed significantly in response to nitrate.

Bottom Line: The results showed that NaCl had a synergetic effect with nitrate on the growth of S. europaea.The application of the Ca(2+) channel blocker LaCl3 not only caused a decrease in nitrate uptake rate, but also attenuated the promoting effects of NaCl on nitrate uptake rates.Based on these results, a possible regulatory network of NaCl-facilitated nitrate uptake in S. europaea focusing on the involvement of Ca(2+) signalling was proposed.

View Article: PubMed Central - PubMed

Affiliation: Key Laboratory of Plant Molecular Physiology, Institute of Botany, Chinese Academy of Sciences, Beijing 100093, PR China.

No MeSH data available.


Related in: MedlinePlus