Limits...
Comparative proteomics of root plasma membrane proteins reveals the involvement of calcium signalling in NaCl-facilitated nitrate uptake in Salicornia europaea.

Nie L, Feng J, Fan P, Chen X, Guo J, Lv S, Bao H, Jia W, Tai F, Jiang P, Wang J, Li Y - J. Exp. Bot. (2015)

Bottom Line: The results showed that NaCl had a synergetic effect with nitrate on the growth of S. europaea.The application of the Ca(2+) channel blocker LaCl3 not only caused a decrease in nitrate uptake rate, but also attenuated the promoting effects of NaCl on nitrate uptake rates.Based on these results, a possible regulatory network of NaCl-facilitated nitrate uptake in S. europaea focusing on the involvement of Ca(2+) signalling was proposed.

View Article: PubMed Central - PubMed

Affiliation: Key Laboratory of Plant Molecular Physiology, Institute of Botany, Chinese Academy of Sciences, Beijing 100093, PR China.

No MeSH data available.


Related in: MedlinePlus

Purity assessment and a representative 2D-DIGE image. (A) Western blot analysis of extracted PM proteins with antibodies against proteins from different cell compartments. (B) PM proteins were separated by 2D-DIGE and detected with a Typhoon 9400 Scanner. The differentially accumulated protein spots under different NaCl and nitrate concentrations were determined using two-way ANOVA, and those positively identified by MALDI-TOF-TOF are marked by arrows.
© Copyright Policy - creative-commons
Related In: Results  -  Collection

License 1 - License 2
getmorefigures.php?uid=PMC4507759&req=5

Figure 3: Purity assessment and a representative 2D-DIGE image. (A) Western blot analysis of extracted PM proteins with antibodies against proteins from different cell compartments. (B) PM proteins were separated by 2D-DIGE and detected with a Typhoon 9400 Scanner. The differentially accumulated protein spots under different NaCl and nitrate concentrations were determined using two-way ANOVA, and those positively identified by MALDI-TOF-TOF are marked by arrows.

Mentions: To determine the proteins regulated by NaCl and nitrate in S. europaea, a comparative proteomic analysis of root PM proteins under the four treatments was conducted. Root PM fractions were purified by the two-phase partitioning method (Tang, 2012), and their purity was evaluated by western blot analysis. Compared with the TM fraction, H+-ATPase was strongly enriched in the PM fraction. Conversely, V-ATPase, a tonoplast membrane protein, was more strongly enriched in the TM fraction than in the PM fraction. Sar1, an integral ER membrane protein, was only detected in the TM fraction and was absent in the PM fraction (Fig. 3A). Furthermore, the hydrolytic activity of PM H+-ATPase in the presence or absence of 0.1mM of the specific PM H+-ATPase inhibitor Na3VO4 was determined, and the purity of PM vesicles was 84.76±2.80%. These results demonstrated that PM vesicles of relatively high purity were obtained.


Comparative proteomics of root plasma membrane proteins reveals the involvement of calcium signalling in NaCl-facilitated nitrate uptake in Salicornia europaea.

Nie L, Feng J, Fan P, Chen X, Guo J, Lv S, Bao H, Jia W, Tai F, Jiang P, Wang J, Li Y - J. Exp. Bot. (2015)

Purity assessment and a representative 2D-DIGE image. (A) Western blot analysis of extracted PM proteins with antibodies against proteins from different cell compartments. (B) PM proteins were separated by 2D-DIGE and detected with a Typhoon 9400 Scanner. The differentially accumulated protein spots under different NaCl and nitrate concentrations were determined using two-way ANOVA, and those positively identified by MALDI-TOF-TOF are marked by arrows.
© Copyright Policy - creative-commons
Related In: Results  -  Collection

License 1 - License 2
Show All Figures
getmorefigures.php?uid=PMC4507759&req=5

Figure 3: Purity assessment and a representative 2D-DIGE image. (A) Western blot analysis of extracted PM proteins with antibodies against proteins from different cell compartments. (B) PM proteins were separated by 2D-DIGE and detected with a Typhoon 9400 Scanner. The differentially accumulated protein spots under different NaCl and nitrate concentrations were determined using two-way ANOVA, and those positively identified by MALDI-TOF-TOF are marked by arrows.
Mentions: To determine the proteins regulated by NaCl and nitrate in S. europaea, a comparative proteomic analysis of root PM proteins under the four treatments was conducted. Root PM fractions were purified by the two-phase partitioning method (Tang, 2012), and their purity was evaluated by western blot analysis. Compared with the TM fraction, H+-ATPase was strongly enriched in the PM fraction. Conversely, V-ATPase, a tonoplast membrane protein, was more strongly enriched in the TM fraction than in the PM fraction. Sar1, an integral ER membrane protein, was only detected in the TM fraction and was absent in the PM fraction (Fig. 3A). Furthermore, the hydrolytic activity of PM H+-ATPase in the presence or absence of 0.1mM of the specific PM H+-ATPase inhibitor Na3VO4 was determined, and the purity of PM vesicles was 84.76±2.80%. These results demonstrated that PM vesicles of relatively high purity were obtained.

Bottom Line: The results showed that NaCl had a synergetic effect with nitrate on the growth of S. europaea.The application of the Ca(2+) channel blocker LaCl3 not only caused a decrease in nitrate uptake rate, but also attenuated the promoting effects of NaCl on nitrate uptake rates.Based on these results, a possible regulatory network of NaCl-facilitated nitrate uptake in S. europaea focusing on the involvement of Ca(2+) signalling was proposed.

View Article: PubMed Central - PubMed

Affiliation: Key Laboratory of Plant Molecular Physiology, Institute of Botany, Chinese Academy of Sciences, Beijing 100093, PR China.

No MeSH data available.


Related in: MedlinePlus