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Comparative proteomics of root plasma membrane proteins reveals the involvement of calcium signalling in NaCl-facilitated nitrate uptake in Salicornia europaea.

Nie L, Feng J, Fan P, Chen X, Guo J, Lv S, Bao H, Jia W, Tai F, Jiang P, Wang J, Li Y - J. Exp. Bot. (2015)

Bottom Line: The results showed that NaCl had a synergetic effect with nitrate on the growth of S. europaea.The application of the Ca(2+) channel blocker LaCl3 not only caused a decrease in nitrate uptake rate, but also attenuated the promoting effects of NaCl on nitrate uptake rates.Based on these results, a possible regulatory network of NaCl-facilitated nitrate uptake in S. europaea focusing on the involvement of Ca(2+) signalling was proposed.

View Article: PubMed Central - PubMed

Affiliation: Key Laboratory of Plant Molecular Physiology, Institute of Botany, Chinese Academy of Sciences, Beijing 100093, PR China.

No MeSH data available.


Total Na and N contents, shoot N and NO3– concentrations, nitrate uptake rates, and NR activities of S. europaea grown under different NaCl and NO3– concentrations. S. europaea seedlings were treated with different nitrate and NaCl concentrations for 30 d, total Na (A) and total N (B) content of the whole plant, shoot N (C) and NO3– (D) concentration, and NR activity (F) were measured. (E) S. europaea seedlings were treated with four different NaCl and 15N labelled K15NO3 concentrations for 15min, and the content of 15N in roots was measured. Values were means ±SE (n=4 for A, B, C, D, and F; and n=3 for E). Asterisks indicate significant differences (P<0.05) between salt-treated and untreated samples. In addition, two-way ANOVA was performed to indicate significant S, N, and S×N effects. Double asterisks indicate significant differences at P<0.01, and combined effects are symbolized by a slash (/). Synergistic, additive, and antagonistic represent different types of interaction.
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Figure 2: Total Na and N contents, shoot N and NO3– concentrations, nitrate uptake rates, and NR activities of S. europaea grown under different NaCl and NO3– concentrations. S. europaea seedlings were treated with different nitrate and NaCl concentrations for 30 d, total Na (A) and total N (B) content of the whole plant, shoot N (C) and NO3– (D) concentration, and NR activity (F) were measured. (E) S. europaea seedlings were treated with four different NaCl and 15N labelled K15NO3 concentrations for 15min, and the content of 15N in roots was measured. Values were means ±SE (n=4 for A, B, C, D, and F; and n=3 for E). Asterisks indicate significant differences (P<0.05) between salt-treated and untreated samples. In addition, two-way ANOVA was performed to indicate significant S, N, and S×N effects. Double asterisks indicate significant differences at P<0.01, and combined effects are symbolized by a slash (/). Synergistic, additive, and antagonistic represent different types of interaction.

Mentions: In order to investigate the effects of NaCl on the nitrate uptake and assimilation, the total Na and total N contents per plant, the N and NO3– concentrations in shoot of S. europaea, the nitrate uptake rates, and nitrate reductase activities were measured (Fig. 2). All of the traits except NR activities showed significant S, N, and S×N effects. As expected, total Na contents were significantly increased under NaCl treatments, which were also promoted by N treatment (Fig. 2A). Under low N conditions, no significant effect of NaCl on total N content and shoot N concentrations was observed (Fig. 2B, C), but the shoot NO3– concentrations and the nitrate uptake rates increased significantly by NaCl treatment. The values increased by 83.4% and 32.8% (Fig. 2D, E), respectively. Compared with the LN condition, NR activity under LN+S increased by 23.5%, but the values were very low and the difference was not statistically significant (Fig. 2F). Under high N conditions, the shoot N concentrations were significantly decreased by NaCl treatment (Fig. 2C), but the total N contents, shoot NO3– concentration, the nitrate uptake rates, and the NR activities were all significantly increased by NaCl treatment. The values increased by 27.8, 14.6, 57.6, and 41.7%, respectively (Fig. 2B–F). Notably, NaCl and NO3– also had a synergistic interaction effect on nitrate uptake rate. These results showed that NaCl could promote nitrate uptake and assimilation in S. europaea.


Comparative proteomics of root plasma membrane proteins reveals the involvement of calcium signalling in NaCl-facilitated nitrate uptake in Salicornia europaea.

Nie L, Feng J, Fan P, Chen X, Guo J, Lv S, Bao H, Jia W, Tai F, Jiang P, Wang J, Li Y - J. Exp. Bot. (2015)

Total Na and N contents, shoot N and NO3– concentrations, nitrate uptake rates, and NR activities of S. europaea grown under different NaCl and NO3– concentrations. S. europaea seedlings were treated with different nitrate and NaCl concentrations for 30 d, total Na (A) and total N (B) content of the whole plant, shoot N (C) and NO3– (D) concentration, and NR activity (F) were measured. (E) S. europaea seedlings were treated with four different NaCl and 15N labelled K15NO3 concentrations for 15min, and the content of 15N in roots was measured. Values were means ±SE (n=4 for A, B, C, D, and F; and n=3 for E). Asterisks indicate significant differences (P<0.05) between salt-treated and untreated samples. In addition, two-way ANOVA was performed to indicate significant S, N, and S×N effects. Double asterisks indicate significant differences at P<0.01, and combined effects are symbolized by a slash (/). Synergistic, additive, and antagonistic represent different types of interaction.
© Copyright Policy - creative-commons
Related In: Results  -  Collection

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Figure 2: Total Na and N contents, shoot N and NO3– concentrations, nitrate uptake rates, and NR activities of S. europaea grown under different NaCl and NO3– concentrations. S. europaea seedlings were treated with different nitrate and NaCl concentrations for 30 d, total Na (A) and total N (B) content of the whole plant, shoot N (C) and NO3– (D) concentration, and NR activity (F) were measured. (E) S. europaea seedlings were treated with four different NaCl and 15N labelled K15NO3 concentrations for 15min, and the content of 15N in roots was measured. Values were means ±SE (n=4 for A, B, C, D, and F; and n=3 for E). Asterisks indicate significant differences (P<0.05) between salt-treated and untreated samples. In addition, two-way ANOVA was performed to indicate significant S, N, and S×N effects. Double asterisks indicate significant differences at P<0.01, and combined effects are symbolized by a slash (/). Synergistic, additive, and antagonistic represent different types of interaction.
Mentions: In order to investigate the effects of NaCl on the nitrate uptake and assimilation, the total Na and total N contents per plant, the N and NO3– concentrations in shoot of S. europaea, the nitrate uptake rates, and nitrate reductase activities were measured (Fig. 2). All of the traits except NR activities showed significant S, N, and S×N effects. As expected, total Na contents were significantly increased under NaCl treatments, which were also promoted by N treatment (Fig. 2A). Under low N conditions, no significant effect of NaCl on total N content and shoot N concentrations was observed (Fig. 2B, C), but the shoot NO3– concentrations and the nitrate uptake rates increased significantly by NaCl treatment. The values increased by 83.4% and 32.8% (Fig. 2D, E), respectively. Compared with the LN condition, NR activity under LN+S increased by 23.5%, but the values were very low and the difference was not statistically significant (Fig. 2F). Under high N conditions, the shoot N concentrations were significantly decreased by NaCl treatment (Fig. 2C), but the total N contents, shoot NO3– concentration, the nitrate uptake rates, and the NR activities were all significantly increased by NaCl treatment. The values increased by 27.8, 14.6, 57.6, and 41.7%, respectively (Fig. 2B–F). Notably, NaCl and NO3– also had a synergistic interaction effect on nitrate uptake rate. These results showed that NaCl could promote nitrate uptake and assimilation in S. europaea.

Bottom Line: The results showed that NaCl had a synergetic effect with nitrate on the growth of S. europaea.The application of the Ca(2+) channel blocker LaCl3 not only caused a decrease in nitrate uptake rate, but also attenuated the promoting effects of NaCl on nitrate uptake rates.Based on these results, a possible regulatory network of NaCl-facilitated nitrate uptake in S. europaea focusing on the involvement of Ca(2+) signalling was proposed.

View Article: PubMed Central - PubMed

Affiliation: Key Laboratory of Plant Molecular Physiology, Institute of Botany, Chinese Academy of Sciences, Beijing 100093, PR China.

No MeSH data available.