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Comparative proteomics of root plasma membrane proteins reveals the involvement of calcium signalling in NaCl-facilitated nitrate uptake in Salicornia europaea.

Nie L, Feng J, Fan P, Chen X, Guo J, Lv S, Bao H, Jia W, Tai F, Jiang P, Wang J, Li Y - J. Exp. Bot. (2015)

Bottom Line: The results showed that NaCl had a synergetic effect with nitrate on the growth of S. europaea.The application of the Ca(2+) channel blocker LaCl3 not only caused a decrease in nitrate uptake rate, but also attenuated the promoting effects of NaCl on nitrate uptake rates.Based on these results, a possible regulatory network of NaCl-facilitated nitrate uptake in S. europaea focusing on the involvement of Ca(2+) signalling was proposed.

View Article: PubMed Central - PubMed

Affiliation: Key Laboratory of Plant Molecular Physiology, Institute of Botany, Chinese Academy of Sciences, Beijing 100093, PR China.

No MeSH data available.


Phenotypic and physiological changes of S. europaea grown under different NaCl and NO3– concentrations. S. europaea seedlings were treated with different nitrate and NaCl concentrations for 30 d, and the phenotypes (A and B), fresh weight (C), dry weight (D), root length (E), and root volume (F) were determined. Scale bars=2cm. Values were means ±SE (n=12). Different letters above the bars indicated significant differences (P<0.05). For C, D, E, and F, two-way ANOVA was performed to indicate significant S, N, and S×N effects. Double asterisks indicate significant differences at P<0.01. Combined effects are symbolized by a slash (/). Synergistic indicates the type of interaction.
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Figure 1: Phenotypic and physiological changes of S. europaea grown under different NaCl and NO3– concentrations. S. europaea seedlings were treated with different nitrate and NaCl concentrations for 30 d, and the phenotypes (A and B), fresh weight (C), dry weight (D), root length (E), and root volume (F) were determined. Scale bars=2cm. Values were means ±SE (n=12). Different letters above the bars indicated significant differences (P<0.05). For C, D, E, and F, two-way ANOVA was performed to indicate significant S, N, and S×N effects. Double asterisks indicate significant differences at P<0.01. Combined effects are symbolized by a slash (/). Synergistic indicates the type of interaction.

Mentions: In this study, the effect of NaCl and nitrate on the growth of S. europaea was first investigated. Seedlings were treated with four different solutions, LN, LN+S, HN, and HN+S. Generally, NaCl has a positive influence on the growth of euhalophytes, for which zero NaCl is an abnormal condition whereas 200mM NaCl is in the range of optimal condition (Flowers and Colmer, 2008). Consistently, in this study, it was found that S. europaea grows better in the presence of 200mM NaCl under both LN and HN conditions. In addition, high N also promotes the growth of S. europaea (Fig. 1A, B). The FW, DW, root length, and root volume were measured, and two-way ANOVA (P<0.05) was used to reveal the effects of S, N, and S×N on these parameters. All the four traits showed significant S and N effects. The FW and DW increased under NaCl and NO3– treatments, and seedlings treated with HN+S exhibited the highest values, whereas LN showed the lowest (Fig. 1C, D). Moreover, a significant S×N interaction was observed for FW and DW, and the type of interaction was synergistic (Fig. 1C, D), suggesting that NaCl and nitrate had synergistic effects on the growth of S. europaea. NaCl facilitated the root growth of S. europaea, whereas high nitrate inhibited it. Both the root length and volume were largest under LN+S treatment, the values of which were 110.8% and 103.1% higher than those treated with HN, respectively (Fig. 1E, F).


Comparative proteomics of root plasma membrane proteins reveals the involvement of calcium signalling in NaCl-facilitated nitrate uptake in Salicornia europaea.

Nie L, Feng J, Fan P, Chen X, Guo J, Lv S, Bao H, Jia W, Tai F, Jiang P, Wang J, Li Y - J. Exp. Bot. (2015)

Phenotypic and physiological changes of S. europaea grown under different NaCl and NO3– concentrations. S. europaea seedlings were treated with different nitrate and NaCl concentrations for 30 d, and the phenotypes (A and B), fresh weight (C), dry weight (D), root length (E), and root volume (F) were determined. Scale bars=2cm. Values were means ±SE (n=12). Different letters above the bars indicated significant differences (P<0.05). For C, D, E, and F, two-way ANOVA was performed to indicate significant S, N, and S×N effects. Double asterisks indicate significant differences at P<0.01. Combined effects are symbolized by a slash (/). Synergistic indicates the type of interaction.
© Copyright Policy - creative-commons
Related In: Results  -  Collection

License 1 - License 2
Show All Figures
getmorefigures.php?uid=PMC4507759&req=5

Figure 1: Phenotypic and physiological changes of S. europaea grown under different NaCl and NO3– concentrations. S. europaea seedlings were treated with different nitrate and NaCl concentrations for 30 d, and the phenotypes (A and B), fresh weight (C), dry weight (D), root length (E), and root volume (F) were determined. Scale bars=2cm. Values were means ±SE (n=12). Different letters above the bars indicated significant differences (P<0.05). For C, D, E, and F, two-way ANOVA was performed to indicate significant S, N, and S×N effects. Double asterisks indicate significant differences at P<0.01. Combined effects are symbolized by a slash (/). Synergistic indicates the type of interaction.
Mentions: In this study, the effect of NaCl and nitrate on the growth of S. europaea was first investigated. Seedlings were treated with four different solutions, LN, LN+S, HN, and HN+S. Generally, NaCl has a positive influence on the growth of euhalophytes, for which zero NaCl is an abnormal condition whereas 200mM NaCl is in the range of optimal condition (Flowers and Colmer, 2008). Consistently, in this study, it was found that S. europaea grows better in the presence of 200mM NaCl under both LN and HN conditions. In addition, high N also promotes the growth of S. europaea (Fig. 1A, B). The FW, DW, root length, and root volume were measured, and two-way ANOVA (P<0.05) was used to reveal the effects of S, N, and S×N on these parameters. All the four traits showed significant S and N effects. The FW and DW increased under NaCl and NO3– treatments, and seedlings treated with HN+S exhibited the highest values, whereas LN showed the lowest (Fig. 1C, D). Moreover, a significant S×N interaction was observed for FW and DW, and the type of interaction was synergistic (Fig. 1C, D), suggesting that NaCl and nitrate had synergistic effects on the growth of S. europaea. NaCl facilitated the root growth of S. europaea, whereas high nitrate inhibited it. Both the root length and volume were largest under LN+S treatment, the values of which were 110.8% and 103.1% higher than those treated with HN, respectively (Fig. 1E, F).

Bottom Line: The results showed that NaCl had a synergetic effect with nitrate on the growth of S. europaea.The application of the Ca(2+) channel blocker LaCl3 not only caused a decrease in nitrate uptake rate, but also attenuated the promoting effects of NaCl on nitrate uptake rates.Based on these results, a possible regulatory network of NaCl-facilitated nitrate uptake in S. europaea focusing on the involvement of Ca(2+) signalling was proposed.

View Article: PubMed Central - PubMed

Affiliation: Key Laboratory of Plant Molecular Physiology, Institute of Botany, Chinese Academy of Sciences, Beijing 100093, PR China.

No MeSH data available.