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A G protein alpha mutation confers prolificacy potential in maize.

Urano D, Jackson D, Jones AM - J. Exp. Bot. (2015)

Bottom Line: The ct2 mutant partially compensated for a reduced shoot height by increased total leaf number, and had far more ears, even in the presence of pollination signals.The maize heterotrimeric G protein complex is important in some plastic developmental traits in maize.In particular, the maize Gα subunit is required to dampen the overproduction of female inflorescences.

View Article: PubMed Central - PubMed

Affiliation: Department of Biology, The University of North Carolina, Chapel Hill, Coker Hall, NC 27599-3280, USA.

No MeSH data available.


A Gα- line decreases longitudinal growth in shoots and roots. (A) Five-week-old seedlings of B73 and the Gα- ct2 mutant. Scale bar=20cm. (B, C) Leaf length and width of B73 and ct2. Panels show raw values of B73 (orange dots, n=5) and ct2 (blue dots, n=4) with a curve fitted by the Gaussian distribution function. (D) Representative roots of 16-d-old B73 and ct2 seedlings grown in 2.0 l Erlenmeyer flasks. A scale shows 5cm. (E, F, G) The longest crown root length (E) was measured on the 14th day, and number of crown roots (F) and seminal roots (G) were measured on the 20th day. Panels show raw values of B73 (orange dots, n=16) and ct2 (blue dots, n=16). Bars represent the means with standard errors of the mean. * or **, respectively, signifies a significant difference between B73 and ct2 groups at the P value less than 0.05 or 0.01, by the two-tailed Student’s t test. n.s. signifies no significant difference at the P value of 0.05. Quantitated values are presented in Supplementary Table S1 at JXB online.
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Figure 1: A Gα- line decreases longitudinal growth in shoots and roots. (A) Five-week-old seedlings of B73 and the Gα- ct2 mutant. Scale bar=20cm. (B, C) Leaf length and width of B73 and ct2. Panels show raw values of B73 (orange dots, n=5) and ct2 (blue dots, n=4) with a curve fitted by the Gaussian distribution function. (D) Representative roots of 16-d-old B73 and ct2 seedlings grown in 2.0 l Erlenmeyer flasks. A scale shows 5cm. (E, F, G) The longest crown root length (E) was measured on the 14th day, and number of crown roots (F) and seminal roots (G) were measured on the 20th day. Panels show raw values of B73 (orange dots, n=16) and ct2 (blue dots, n=16). Bars represent the means with standard errors of the mean. * or **, respectively, signifies a significant difference between B73 and ct2 groups at the P value less than 0.05 or 0.01, by the two-tailed Student’s t test. n.s. signifies no significant difference at the P value of 0.05. Quantitated values are presented in Supplementary Table S1 at JXB online.

Mentions: The leaf shape of the ct2 mutants was noticeably different (Fig. 1A; 5-week-old plants of B73 and ct2 grown in the greenhouse). The ct2 mutation led to a shortening of the leaf blade by 18–42% in all leaves (Fig. 1B) and plant height by 32% (see Supplementary Fig. S1A, B at JXB online), while slightly increasing leaf width (Fig. 1C). The ct2 plants also had an increased number of leaves per plant (B73, 18.4 leaves; ct2, 20.0 leaves), and a slightly delayed growth rate of leaves (see Supplementary Fig. S1C, D at JXB online). The total leaf area of B73 was 8369cm2 (n=5), compared with ct2 total leaf area of 6515cm2 (n=4), 22% less than the wild type. Thus the increased number of leaves in ct2 mutants did not fully compensate the reduced individual leaf area.


A G protein alpha mutation confers prolificacy potential in maize.

Urano D, Jackson D, Jones AM - J. Exp. Bot. (2015)

A Gα- line decreases longitudinal growth in shoots and roots. (A) Five-week-old seedlings of B73 and the Gα- ct2 mutant. Scale bar=20cm. (B, C) Leaf length and width of B73 and ct2. Panels show raw values of B73 (orange dots, n=5) and ct2 (blue dots, n=4) with a curve fitted by the Gaussian distribution function. (D) Representative roots of 16-d-old B73 and ct2 seedlings grown in 2.0 l Erlenmeyer flasks. A scale shows 5cm. (E, F, G) The longest crown root length (E) was measured on the 14th day, and number of crown roots (F) and seminal roots (G) were measured on the 20th day. Panels show raw values of B73 (orange dots, n=16) and ct2 (blue dots, n=16). Bars represent the means with standard errors of the mean. * or **, respectively, signifies a significant difference between B73 and ct2 groups at the P value less than 0.05 or 0.01, by the two-tailed Student’s t test. n.s. signifies no significant difference at the P value of 0.05. Quantitated values are presented in Supplementary Table S1 at JXB online.
© Copyright Policy - creative-commons
Related In: Results  -  Collection

License 1 - License 2
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Figure 1: A Gα- line decreases longitudinal growth in shoots and roots. (A) Five-week-old seedlings of B73 and the Gα- ct2 mutant. Scale bar=20cm. (B, C) Leaf length and width of B73 and ct2. Panels show raw values of B73 (orange dots, n=5) and ct2 (blue dots, n=4) with a curve fitted by the Gaussian distribution function. (D) Representative roots of 16-d-old B73 and ct2 seedlings grown in 2.0 l Erlenmeyer flasks. A scale shows 5cm. (E, F, G) The longest crown root length (E) was measured on the 14th day, and number of crown roots (F) and seminal roots (G) were measured on the 20th day. Panels show raw values of B73 (orange dots, n=16) and ct2 (blue dots, n=16). Bars represent the means with standard errors of the mean. * or **, respectively, signifies a significant difference between B73 and ct2 groups at the P value less than 0.05 or 0.01, by the two-tailed Student’s t test. n.s. signifies no significant difference at the P value of 0.05. Quantitated values are presented in Supplementary Table S1 at JXB online.
Mentions: The leaf shape of the ct2 mutants was noticeably different (Fig. 1A; 5-week-old plants of B73 and ct2 grown in the greenhouse). The ct2 mutation led to a shortening of the leaf blade by 18–42% in all leaves (Fig. 1B) and plant height by 32% (see Supplementary Fig. S1A, B at JXB online), while slightly increasing leaf width (Fig. 1C). The ct2 plants also had an increased number of leaves per plant (B73, 18.4 leaves; ct2, 20.0 leaves), and a slightly delayed growth rate of leaves (see Supplementary Fig. S1C, D at JXB online). The total leaf area of B73 was 8369cm2 (n=5), compared with ct2 total leaf area of 6515cm2 (n=4), 22% less than the wild type. Thus the increased number of leaves in ct2 mutants did not fully compensate the reduced individual leaf area.

Bottom Line: The ct2 mutant partially compensated for a reduced shoot height by increased total leaf number, and had far more ears, even in the presence of pollination signals.The maize heterotrimeric G protein complex is important in some plastic developmental traits in maize.In particular, the maize Gα subunit is required to dampen the overproduction of female inflorescences.

View Article: PubMed Central - PubMed

Affiliation: Department of Biology, The University of North Carolina, Chapel Hill, Coker Hall, NC 27599-3280, USA.

No MeSH data available.