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Amylopectin biosynthetic enzymes from developing rice seed form enzymatically active protein complexes.

Crofts N, Abe N, Oitome NF, Matsushima R, Hayashi M, Tetlow IJ, Emes MJ, Nakamura Y, Fujita N - J. Exp. Bot. (2015)

Bottom Line: This study investigated whether protein-protein interactions are also found in rice endosperm, as well as exploring differences between species.Blue-native-PAGE zymogram analyses confirmed the glucan-synthesizing activity of protein complexes.These results suggest that some rice starch biosynthetic isozymes are physically associated with each other and form active protein complexes.

View Article: PubMed Central - PubMed

Affiliation: Department of Biological Production, Akita Prefectural University, 241-438 Kaidobata-Nishi, Shimoshinjo-Nakano, Akita city, Akita 010-0195, Japan.

No MeSH data available.


Related in: MedlinePlus

Western blots of BN-PAGE shows the formation of starch biosynthetic enzyme complexes from developing rice endosperm. Arrowheads and brackets indicate the presence of polypeptides recognized by antibodies. Double asterisks indicate the co-migrating enzymes. (This figure is available in colour at JXB online.)
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Figure 5: Western blots of BN-PAGE shows the formation of starch biosynthetic enzyme complexes from developing rice endosperm. Arrowheads and brackets indicate the presence of polypeptides recognized by antibodies. Double asterisks indicate the co-migrating enzymes. (This figure is available in colour at JXB online.)

Mentions: The presence of starch biosynthetic enzymes in high molecular weight complexes was confirmed by western blotting of a BN-PAGE gel (Fig. 5) and two-dimensional gels in which BN-PAGE and SDS–PAGE were used for the first and second dimension, respectively (Supplementary Fig. S3 at JXB online). Western blotting of BN-PAGE (Fig. 5) was performed using slices of lanes from a single gel so the molecular weight of proteins is directly comparable. Western blotting of two dimensional gels (Supplementary Fig. S3) showed that the enzymes present at high molecular weight on BN-PAGE (Fig. 5) were present as protein complexes since they migrated to their expected monomeric molecular weight in two-dimensional gels.


Amylopectin biosynthetic enzymes from developing rice seed form enzymatically active protein complexes.

Crofts N, Abe N, Oitome NF, Matsushima R, Hayashi M, Tetlow IJ, Emes MJ, Nakamura Y, Fujita N - J. Exp. Bot. (2015)

Western blots of BN-PAGE shows the formation of starch biosynthetic enzyme complexes from developing rice endosperm. Arrowheads and brackets indicate the presence of polypeptides recognized by antibodies. Double asterisks indicate the co-migrating enzymes. (This figure is available in colour at JXB online.)
© Copyright Policy - creative-commons
Related In: Results  -  Collection

License 1 - License 2
Show All Figures
getmorefigures.php?uid=PMC4507757&req=5

Figure 5: Western blots of BN-PAGE shows the formation of starch biosynthetic enzyme complexes from developing rice endosperm. Arrowheads and brackets indicate the presence of polypeptides recognized by antibodies. Double asterisks indicate the co-migrating enzymes. (This figure is available in colour at JXB online.)
Mentions: The presence of starch biosynthetic enzymes in high molecular weight complexes was confirmed by western blotting of a BN-PAGE gel (Fig. 5) and two-dimensional gels in which BN-PAGE and SDS–PAGE were used for the first and second dimension, respectively (Supplementary Fig. S3 at JXB online). Western blotting of BN-PAGE (Fig. 5) was performed using slices of lanes from a single gel so the molecular weight of proteins is directly comparable. Western blotting of two dimensional gels (Supplementary Fig. S3) showed that the enzymes present at high molecular weight on BN-PAGE (Fig. 5) were present as protein complexes since they migrated to their expected monomeric molecular weight in two-dimensional gels.

Bottom Line: This study investigated whether protein-protein interactions are also found in rice endosperm, as well as exploring differences between species.Blue-native-PAGE zymogram analyses confirmed the glucan-synthesizing activity of protein complexes.These results suggest that some rice starch biosynthetic isozymes are physically associated with each other and form active protein complexes.

View Article: PubMed Central - PubMed

Affiliation: Department of Biological Production, Akita Prefectural University, 241-438 Kaidobata-Nishi, Shimoshinjo-Nakano, Akita city, Akita 010-0195, Japan.

No MeSH data available.


Related in: MedlinePlus