Limits...
Amylopectin biosynthetic enzymes from developing rice seed form enzymatically active protein complexes.

Crofts N, Abe N, Oitome NF, Matsushima R, Hayashi M, Tetlow IJ, Emes MJ, Nakamura Y, Fujita N - J. Exp. Bot. (2015)

Bottom Line: This study investigated whether protein-protein interactions are also found in rice endosperm, as well as exploring differences between species.Blue-native-PAGE zymogram analyses confirmed the glucan-synthesizing activity of protein complexes.These results suggest that some rice starch biosynthetic isozymes are physically associated with each other and form active protein complexes.

View Article: PubMed Central - PubMed

Affiliation: Department of Biological Production, Akita Prefectural University, 241-438 Kaidobata-Nishi, Shimoshinjo-Nakano, Akita city, Akita 010-0195, Japan.

No MeSH data available.


Related in: MedlinePlus

Analyses of protein–protein interaction between rice starch biosynthetic isozymes by co-immunoprecipitation (IP). Soluble proteins from rice developing endosperm were immunoprecipitated using antibodies as described. Pre-immune serum was used as a control. The antibodies used for western blots are indicated on the right. Asterisks indicate the interactions confirmed by reciprocal IP.
© Copyright Policy - creative-commons
Related In: Results  -  Collection

License 1 - License 2
getmorefigures.php?uid=PMC4507757&req=5

Figure 4: Analyses of protein–protein interaction between rice starch biosynthetic isozymes by co-immunoprecipitation (IP). Soluble proteins from rice developing endosperm were immunoprecipitated using antibodies as described. Pre-immune serum was used as a control. The antibodies used for western blots are indicated on the right. Asterisks indicate the interactions confirmed by reciprocal IP.

Mentions: In order to investigate possible interacting partners among starch biosynthetic isozymes, immunoprecipitation was carried out using soluble rice endosperm extract and isozyme-specific antibodies or a pre-immune serum control (Fig. 4, and summarized in Table 2). Each antibody recognized and could immunoprecipitate its respective antigen, except for anti-ISA1 antibodies which could not immunoprecipitate the native protein (results not shown).


Amylopectin biosynthetic enzymes from developing rice seed form enzymatically active protein complexes.

Crofts N, Abe N, Oitome NF, Matsushima R, Hayashi M, Tetlow IJ, Emes MJ, Nakamura Y, Fujita N - J. Exp. Bot. (2015)

Analyses of protein–protein interaction between rice starch biosynthetic isozymes by co-immunoprecipitation (IP). Soluble proteins from rice developing endosperm were immunoprecipitated using antibodies as described. Pre-immune serum was used as a control. The antibodies used for western blots are indicated on the right. Asterisks indicate the interactions confirmed by reciprocal IP.
© Copyright Policy - creative-commons
Related In: Results  -  Collection

License 1 - License 2
Show All Figures
getmorefigures.php?uid=PMC4507757&req=5

Figure 4: Analyses of protein–protein interaction between rice starch biosynthetic isozymes by co-immunoprecipitation (IP). Soluble proteins from rice developing endosperm were immunoprecipitated using antibodies as described. Pre-immune serum was used as a control. The antibodies used for western blots are indicated on the right. Asterisks indicate the interactions confirmed by reciprocal IP.
Mentions: In order to investigate possible interacting partners among starch biosynthetic isozymes, immunoprecipitation was carried out using soluble rice endosperm extract and isozyme-specific antibodies or a pre-immune serum control (Fig. 4, and summarized in Table 2). Each antibody recognized and could immunoprecipitate its respective antigen, except for anti-ISA1 antibodies which could not immunoprecipitate the native protein (results not shown).

Bottom Line: This study investigated whether protein-protein interactions are also found in rice endosperm, as well as exploring differences between species.Blue-native-PAGE zymogram analyses confirmed the glucan-synthesizing activity of protein complexes.These results suggest that some rice starch biosynthetic isozymes are physically associated with each other and form active protein complexes.

View Article: PubMed Central - PubMed

Affiliation: Department of Biological Production, Akita Prefectural University, 241-438 Kaidobata-Nishi, Shimoshinjo-Nakano, Akita city, Akita 010-0195, Japan.

No MeSH data available.


Related in: MedlinePlus