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Amylopectin biosynthetic enzymes from developing rice seed form enzymatically active protein complexes.

Crofts N, Abe N, Oitome NF, Matsushima R, Hayashi M, Tetlow IJ, Emes MJ, Nakamura Y, Fujita N - J. Exp. Bot. (2015)

Bottom Line: This study investigated whether protein-protein interactions are also found in rice endosperm, as well as exploring differences between species.Blue-native-PAGE zymogram analyses confirmed the glucan-synthesizing activity of protein complexes.These results suggest that some rice starch biosynthetic isozymes are physically associated with each other and form active protein complexes.

View Article: PubMed Central - PubMed

Affiliation: Department of Biological Production, Akita Prefectural University, 241-438 Kaidobata-Nishi, Shimoshinjo-Nakano, Akita city, Akita 010-0195, Japan.

No MeSH data available.


Related in: MedlinePlus

Molecular weight distributions of starch biosynthetic enzymes from developing rice endosperm determined by gel permeation chromatography. Soluble proteins from rice endosperm were separated on Superdex 200 and fractions analysed by western blotting using the antibodies indicated. The molecular weight of protein standards is shown at the top (black bars). Monomeric molecular weights of each isozyme are indicated on the right.
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Figure 2: Molecular weight distributions of starch biosynthetic enzymes from developing rice endosperm determined by gel permeation chromatography. Soluble proteins from rice endosperm were separated on Superdex 200 and fractions analysed by western blotting using the antibodies indicated. The molecular weight of protein standards is shown at the top (black bars). Monomeric molecular weights of each isozyme are indicated on the right.

Mentions: Soluble extracts from developing seeds were fractionated using a Superdex 200 gel filtration column. Native molecular weight standards were clearly separated by GPC (Fig. 2, black bars). Figure 2 shows that while some proteins could be detected at their expected monomeric size, all starch biosynthetic proteins analysed were eluted at higher molecular weights, consistent with the possibility that they may form higher order complexes.


Amylopectin biosynthetic enzymes from developing rice seed form enzymatically active protein complexes.

Crofts N, Abe N, Oitome NF, Matsushima R, Hayashi M, Tetlow IJ, Emes MJ, Nakamura Y, Fujita N - J. Exp. Bot. (2015)

Molecular weight distributions of starch biosynthetic enzymes from developing rice endosperm determined by gel permeation chromatography. Soluble proteins from rice endosperm were separated on Superdex 200 and fractions analysed by western blotting using the antibodies indicated. The molecular weight of protein standards is shown at the top (black bars). Monomeric molecular weights of each isozyme are indicated on the right.
© Copyright Policy - creative-commons
Related In: Results  -  Collection

License 1 - License 2
Show All Figures
getmorefigures.php?uid=PMC4507757&req=5

Figure 2: Molecular weight distributions of starch biosynthetic enzymes from developing rice endosperm determined by gel permeation chromatography. Soluble proteins from rice endosperm were separated on Superdex 200 and fractions analysed by western blotting using the antibodies indicated. The molecular weight of protein standards is shown at the top (black bars). Monomeric molecular weights of each isozyme are indicated on the right.
Mentions: Soluble extracts from developing seeds were fractionated using a Superdex 200 gel filtration column. Native molecular weight standards were clearly separated by GPC (Fig. 2, black bars). Figure 2 shows that while some proteins could be detected at their expected monomeric size, all starch biosynthetic proteins analysed were eluted at higher molecular weights, consistent with the possibility that they may form higher order complexes.

Bottom Line: This study investigated whether protein-protein interactions are also found in rice endosperm, as well as exploring differences between species.Blue-native-PAGE zymogram analyses confirmed the glucan-synthesizing activity of protein complexes.These results suggest that some rice starch biosynthetic isozymes are physically associated with each other and form active protein complexes.

View Article: PubMed Central - PubMed

Affiliation: Department of Biological Production, Akita Prefectural University, 241-438 Kaidobata-Nishi, Shimoshinjo-Nakano, Akita city, Akita 010-0195, Japan.

No MeSH data available.


Related in: MedlinePlus