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Identification of candidate flavonoid pathway genes using transcriptome correlation network analysis in ripe strawberry (Fragaria × ananassa) fruits.

Pillet J, Yu HW, Chambers AH, Whitaker VM, Folta KM - J. Exp. Bot. (2015)

Bottom Line: The results suggest that two of the newly-identified regulators likely contribute to discrete nodes of the flavonoid pathway.The third putative transcription factor appears to be a universal regulator of flavonoid-pathway genes, as many pathway transcripts decrease in abundance when this gene is silenced.This report demonstrates that such systems-level approaches may be especially powerful when connected to an effective transient expression system, helping to provide rapid and strong evidence of gene function in key fruit-ripening processes.

View Article: PubMed Central - PubMed

Affiliation: Horticultural Sciences Department, University of Florida, Gainesville, FL 32611, USA.

No MeSH data available.


Decreased transcript accumulation in agro-infiltrated fruits with FaSCL8 silencing. Significance was measured by taking in account all the independent fruits compared to all controls. * P<0.05; **, P<0.01. PAL, phenylalanine ammonia lyase; CHS, chalcone synthase; CHI, chalcone isomerase; F3H, flavanone 3 hydrolase; UFGT, UDP-glucose flavonoid glycosyl transferase.
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Figure 7: Decreased transcript accumulation in agro-infiltrated fruits with FaSCL8 silencing. Significance was measured by taking in account all the independent fruits compared to all controls. * P<0.05; **, P<0.01. PAL, phenylalanine ammonia lyase; CHS, chalcone synthase; CHI, chalcone isomerase; F3H, flavanone 3 hydrolase; UFGT, UDP-glucose flavonoid glycosyl transferase.

Mentions: The last candidate gene analysed was FaSCL8. Due to its high expression levels in ripe fruit, RNA silencing was used for functional analysis. Transcript levels were suppressed 3-fold (compared to WT) in three independent fruits (HP 1, 2 and 3) and >15-fold lower in HP 4 (Fig. 7). Silencing FaSCL8 significantly results in lower transcript accumulation of PAL2, CHS1, CHS2, CHI, F3H and UFGT. It also inhibits the expression of MYB10, a TF that regulates the expression of most of the genes involved in anthocyanin production in ripened fruit receptacles. Surprisingly the silencing of FaSCL8 also results in increased transcript accumulation from F3’H and ANR1, suggesting another role of this TF in the regulation of the pathway (Fig. 8). Furthermore it appears that FaSCL8 can influence the expression of MYB9 and MYB11, TFs that can regulate the tannin production in strawberry fruit.


Identification of candidate flavonoid pathway genes using transcriptome correlation network analysis in ripe strawberry (Fragaria × ananassa) fruits.

Pillet J, Yu HW, Chambers AH, Whitaker VM, Folta KM - J. Exp. Bot. (2015)

Decreased transcript accumulation in agro-infiltrated fruits with FaSCL8 silencing. Significance was measured by taking in account all the independent fruits compared to all controls. * P<0.05; **, P<0.01. PAL, phenylalanine ammonia lyase; CHS, chalcone synthase; CHI, chalcone isomerase; F3H, flavanone 3 hydrolase; UFGT, UDP-glucose flavonoid glycosyl transferase.
© Copyright Policy - creative-commons
Related In: Results  -  Collection

License 1 - License 2
Show All Figures
getmorefigures.php?uid=PMC4507756&req=5

Figure 7: Decreased transcript accumulation in agro-infiltrated fruits with FaSCL8 silencing. Significance was measured by taking in account all the independent fruits compared to all controls. * P<0.05; **, P<0.01. PAL, phenylalanine ammonia lyase; CHS, chalcone synthase; CHI, chalcone isomerase; F3H, flavanone 3 hydrolase; UFGT, UDP-glucose flavonoid glycosyl transferase.
Mentions: The last candidate gene analysed was FaSCL8. Due to its high expression levels in ripe fruit, RNA silencing was used for functional analysis. Transcript levels were suppressed 3-fold (compared to WT) in three independent fruits (HP 1, 2 and 3) and >15-fold lower in HP 4 (Fig. 7). Silencing FaSCL8 significantly results in lower transcript accumulation of PAL2, CHS1, CHS2, CHI, F3H and UFGT. It also inhibits the expression of MYB10, a TF that regulates the expression of most of the genes involved in anthocyanin production in ripened fruit receptacles. Surprisingly the silencing of FaSCL8 also results in increased transcript accumulation from F3’H and ANR1, suggesting another role of this TF in the regulation of the pathway (Fig. 8). Furthermore it appears that FaSCL8 can influence the expression of MYB9 and MYB11, TFs that can regulate the tannin production in strawberry fruit.

Bottom Line: The results suggest that two of the newly-identified regulators likely contribute to discrete nodes of the flavonoid pathway.The third putative transcription factor appears to be a universal regulator of flavonoid-pathway genes, as many pathway transcripts decrease in abundance when this gene is silenced.This report demonstrates that such systems-level approaches may be especially powerful when connected to an effective transient expression system, helping to provide rapid and strong evidence of gene function in key fruit-ripening processes.

View Article: PubMed Central - PubMed

Affiliation: Horticultural Sciences Department, University of Florida, Gainesville, FL 32611, USA.

No MeSH data available.