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Identification of candidate flavonoid pathway genes using transcriptome correlation network analysis in ripe strawberry (Fragaria × ananassa) fruits.

Pillet J, Yu HW, Chambers AH, Whitaker VM, Folta KM - J. Exp. Bot. (2015)

Bottom Line: The results suggest that two of the newly-identified regulators likely contribute to discrete nodes of the flavonoid pathway.The third putative transcription factor appears to be a universal regulator of flavonoid-pathway genes, as many pathway transcripts decrease in abundance when this gene is silenced.This report demonstrates that such systems-level approaches may be especially powerful when connected to an effective transient expression system, helping to provide rapid and strong evidence of gene function in key fruit-ripening processes.

View Article: PubMed Central - PubMed

Affiliation: Horticultural Sciences Department, University of Florida, Gainesville, FL 32611, USA.

No MeSH data available.


Expression profiles in agro-infiltrated fruits overexpressing PCL1-like. Significance was measured comparing expression levels in construct-containing, independent fruits compared to all controls. *, P<0.05; **, P<0.01. FLS, flavonol synthase; LAR, leucoanthocyanidin reductase.
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Figure 6: Expression profiles in agro-infiltrated fruits overexpressing PCL1-like. Significance was measured comparing expression levels in construct-containing, independent fruits compared to all controls. *, P<0.05; **, P<0.01. FLS, flavonol synthase; LAR, leucoanthocyanidin reductase.

Mentions: Transient overexpression of FaPCL1-like resulted in transcript levels 10–20-fold higher compared to control fruits (Fig. 6). This overexpression caused a 10–60-fold induction of FLS, and suggests a role of this TF in flavonol synthesis. However, overexpression of FaPCL1-like also induces the expression of LAR from 2–14-fold, and connects this TF to tannin synthesis.


Identification of candidate flavonoid pathway genes using transcriptome correlation network analysis in ripe strawberry (Fragaria × ananassa) fruits.

Pillet J, Yu HW, Chambers AH, Whitaker VM, Folta KM - J. Exp. Bot. (2015)

Expression profiles in agro-infiltrated fruits overexpressing PCL1-like. Significance was measured comparing expression levels in construct-containing, independent fruits compared to all controls. *, P<0.05; **, P<0.01. FLS, flavonol synthase; LAR, leucoanthocyanidin reductase.
© Copyright Policy - creative-commons
Related In: Results  -  Collection

License 1 - License 2
Show All Figures
getmorefigures.php?uid=PMC4507756&req=5

Figure 6: Expression profiles in agro-infiltrated fruits overexpressing PCL1-like. Significance was measured comparing expression levels in construct-containing, independent fruits compared to all controls. *, P<0.05; **, P<0.01. FLS, flavonol synthase; LAR, leucoanthocyanidin reductase.
Mentions: Transient overexpression of FaPCL1-like resulted in transcript levels 10–20-fold higher compared to control fruits (Fig. 6). This overexpression caused a 10–60-fold induction of FLS, and suggests a role of this TF in flavonol synthesis. However, overexpression of FaPCL1-like also induces the expression of LAR from 2–14-fold, and connects this TF to tannin synthesis.

Bottom Line: The results suggest that two of the newly-identified regulators likely contribute to discrete nodes of the flavonoid pathway.The third putative transcription factor appears to be a universal regulator of flavonoid-pathway genes, as many pathway transcripts decrease in abundance when this gene is silenced.This report demonstrates that such systems-level approaches may be especially powerful when connected to an effective transient expression system, helping to provide rapid and strong evidence of gene function in key fruit-ripening processes.

View Article: PubMed Central - PubMed

Affiliation: Horticultural Sciences Department, University of Florida, Gainesville, FL 32611, USA.

No MeSH data available.