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Identification of candidate flavonoid pathway genes using transcriptome correlation network analysis in ripe strawberry (Fragaria × ananassa) fruits.

Pillet J, Yu HW, Chambers AH, Whitaker VM, Folta KM - J. Exp. Bot. (2015)

Bottom Line: The results suggest that two of the newly-identified regulators likely contribute to discrete nodes of the flavonoid pathway.The third putative transcription factor appears to be a universal regulator of flavonoid-pathway genes, as many pathway transcripts decrease in abundance when this gene is silenced.This report demonstrates that such systems-level approaches may be especially powerful when connected to an effective transient expression system, helping to provide rapid and strong evidence of gene function in key fruit-ripening processes.

View Article: PubMed Central - PubMed

Affiliation: Horticultural Sciences Department, University of Florida, Gainesville, FL 32611, USA.

No MeSH data available.


Expression profiles in agro-infiltrated fruits overexpressing FaTCP11. Significance was measured by taking into account the relative transcript accumulation in all of the independent fruits compared to all controls. **, P<0.01; ***, P<0.001. F3’H, flavanone 3’ hydrolase; LAR, leucoanthocyanidin reductase.
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Figure 5: Expression profiles in agro-infiltrated fruits overexpressing FaTCP11. Significance was measured by taking into account the relative transcript accumulation in all of the independent fruits compared to all controls. **, P<0.01; ***, P<0.001. F3’H, flavanone 3’ hydrolase; LAR, leucoanthocyanidin reductase.

Mentions: The effects of misexpression of the candidate transcripts were assessed using transient expression assays in fruit. Agro-infiltration of maturing strawberry fruit has been a useful tool in defining gene contributions to ripening (Hoffmann et al., 2006). First, fruits were inoculated with constructs unrelated to the flavonoid pathway to ensure that the infiltration process alone would not affect the flavonoid pathway (Supplementary Fig. S2). Next, FaTCP11 and FaPCL1-like were overexpressed in ‘Strawberry Festival’. Overexpression assays were performed because these transcripts were expressed at low levels in developing fruit. FaTCP11 was overexpressed 10-fold higher in three independent fruits (OEX 1, 2 and 3) and more than 70-fold higher in one fruit (OEX 4) compared to control fruits (Fig. 5). Analysis of the flavonoid gene expression profiles clearly showed that this gene has an effect on the expression of LAR which is induced 6–9-fold in OEX 1, 2 and 3 and >13-fold in OEX 4. FaTCP11 has also an effect on F3’H as its expression is slightly induced in the agro-infiltrated fruits. These results suggest a putative, yet specific, role of FaTCP11 in the synthesis of flavan-3-ols.


Identification of candidate flavonoid pathway genes using transcriptome correlation network analysis in ripe strawberry (Fragaria × ananassa) fruits.

Pillet J, Yu HW, Chambers AH, Whitaker VM, Folta KM - J. Exp. Bot. (2015)

Expression profiles in agro-infiltrated fruits overexpressing FaTCP11. Significance was measured by taking into account the relative transcript accumulation in all of the independent fruits compared to all controls. **, P<0.01; ***, P<0.001. F3’H, flavanone 3’ hydrolase; LAR, leucoanthocyanidin reductase.
© Copyright Policy - creative-commons
Related In: Results  -  Collection

License 1 - License 2
Show All Figures
getmorefigures.php?uid=PMC4507756&req=5

Figure 5: Expression profiles in agro-infiltrated fruits overexpressing FaTCP11. Significance was measured by taking into account the relative transcript accumulation in all of the independent fruits compared to all controls. **, P<0.01; ***, P<0.001. F3’H, flavanone 3’ hydrolase; LAR, leucoanthocyanidin reductase.
Mentions: The effects of misexpression of the candidate transcripts were assessed using transient expression assays in fruit. Agro-infiltration of maturing strawberry fruit has been a useful tool in defining gene contributions to ripening (Hoffmann et al., 2006). First, fruits were inoculated with constructs unrelated to the flavonoid pathway to ensure that the infiltration process alone would not affect the flavonoid pathway (Supplementary Fig. S2). Next, FaTCP11 and FaPCL1-like were overexpressed in ‘Strawberry Festival’. Overexpression assays were performed because these transcripts were expressed at low levels in developing fruit. FaTCP11 was overexpressed 10-fold higher in three independent fruits (OEX 1, 2 and 3) and more than 70-fold higher in one fruit (OEX 4) compared to control fruits (Fig. 5). Analysis of the flavonoid gene expression profiles clearly showed that this gene has an effect on the expression of LAR which is induced 6–9-fold in OEX 1, 2 and 3 and >13-fold in OEX 4. FaTCP11 has also an effect on F3’H as its expression is slightly induced in the agro-infiltrated fruits. These results suggest a putative, yet specific, role of FaTCP11 in the synthesis of flavan-3-ols.

Bottom Line: The results suggest that two of the newly-identified regulators likely contribute to discrete nodes of the flavonoid pathway.The third putative transcription factor appears to be a universal regulator of flavonoid-pathway genes, as many pathway transcripts decrease in abundance when this gene is silenced.This report demonstrates that such systems-level approaches may be especially powerful when connected to an effective transient expression system, helping to provide rapid and strong evidence of gene function in key fruit-ripening processes.

View Article: PubMed Central - PubMed

Affiliation: Horticultural Sciences Department, University of Florida, Gainesville, FL 32611, USA.

No MeSH data available.