Limits...
Identification of candidate flavonoid pathway genes using transcriptome correlation network analysis in ripe strawberry (Fragaria × ananassa) fruits.

Pillet J, Yu HW, Chambers AH, Whitaker VM, Folta KM - J. Exp. Bot. (2015)

Bottom Line: The results suggest that two of the newly-identified regulators likely contribute to discrete nodes of the flavonoid pathway.The third putative transcription factor appears to be a universal regulator of flavonoid-pathway genes, as many pathway transcripts decrease in abundance when this gene is silenced.This report demonstrates that such systems-level approaches may be especially powerful when connected to an effective transient expression system, helping to provide rapid and strong evidence of gene function in key fruit-ripening processes.

View Article: PubMed Central - PubMed

Affiliation: Horticultural Sciences Department, University of Florida, Gainesville, FL 32611, USA.

No MeSH data available.


Expression patterns of FaTCP11, FaPCL1-like and FaSCL8 during fruit development. GR, green stage receptacle; WR, white stage receptacle; TR, turning stage receptacle; RR, red stage receptacle; GA, green stage achenes; WA, white stage achenes; TA, turning stage achenes; RA, red stage achenes. Significance was measured by a one-way ANOVA with P<0.001.
© Copyright Policy - creative-commons
Related In: Results  -  Collection

License 1 - License 2
getmorefigures.php?uid=PMC4507756&req=5

Figure 4: Expression patterns of FaTCP11, FaPCL1-like and FaSCL8 during fruit development. GR, green stage receptacle; WR, white stage receptacle; TR, turning stage receptacle; RR, red stage receptacle; GA, green stage achenes; WA, white stage achenes; TA, turning stage achenes; RA, red stage achenes. Significance was measured by a one-way ANOVA with P<0.001.

Mentions: To further infer potential function, candidate transcript accumulation was measured in the receptacle and achenes in response to ripening. In the receptacle, FaTCP11 and FaPCL1-like expression is stable during development and decreases during the red stage (Fig. 4). In the achenes, both transcripts are present at their highest levels at the green stage, and then the expression level decreases with a slight increase later in maturity. On the contrary, FaSCL8 expression shows an induction in the receptacle coincident with ripening. FaSCL8 is less expressed in achenes compared to the other flavonoid-associated genes.


Identification of candidate flavonoid pathway genes using transcriptome correlation network analysis in ripe strawberry (Fragaria × ananassa) fruits.

Pillet J, Yu HW, Chambers AH, Whitaker VM, Folta KM - J. Exp. Bot. (2015)

Expression patterns of FaTCP11, FaPCL1-like and FaSCL8 during fruit development. GR, green stage receptacle; WR, white stage receptacle; TR, turning stage receptacle; RR, red stage receptacle; GA, green stage achenes; WA, white stage achenes; TA, turning stage achenes; RA, red stage achenes. Significance was measured by a one-way ANOVA with P<0.001.
© Copyright Policy - creative-commons
Related In: Results  -  Collection

License 1 - License 2
Show All Figures
getmorefigures.php?uid=PMC4507756&req=5

Figure 4: Expression patterns of FaTCP11, FaPCL1-like and FaSCL8 during fruit development. GR, green stage receptacle; WR, white stage receptacle; TR, turning stage receptacle; RR, red stage receptacle; GA, green stage achenes; WA, white stage achenes; TA, turning stage achenes; RA, red stage achenes. Significance was measured by a one-way ANOVA with P<0.001.
Mentions: To further infer potential function, candidate transcript accumulation was measured in the receptacle and achenes in response to ripening. In the receptacle, FaTCP11 and FaPCL1-like expression is stable during development and decreases during the red stage (Fig. 4). In the achenes, both transcripts are present at their highest levels at the green stage, and then the expression level decreases with a slight increase later in maturity. On the contrary, FaSCL8 expression shows an induction in the receptacle coincident with ripening. FaSCL8 is less expressed in achenes compared to the other flavonoid-associated genes.

Bottom Line: The results suggest that two of the newly-identified regulators likely contribute to discrete nodes of the flavonoid pathway.The third putative transcription factor appears to be a universal regulator of flavonoid-pathway genes, as many pathway transcripts decrease in abundance when this gene is silenced.This report demonstrates that such systems-level approaches may be especially powerful when connected to an effective transient expression system, helping to provide rapid and strong evidence of gene function in key fruit-ripening processes.

View Article: PubMed Central - PubMed

Affiliation: Horticultural Sciences Department, University of Florida, Gainesville, FL 32611, USA.

No MeSH data available.