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Identification of candidate flavonoid pathway genes using transcriptome correlation network analysis in ripe strawberry (Fragaria × ananassa) fruits.

Pillet J, Yu HW, Chambers AH, Whitaker VM, Folta KM - J. Exp. Bot. (2015)

Bottom Line: The results suggest that two of the newly-identified regulators likely contribute to discrete nodes of the flavonoid pathway.The third putative transcription factor appears to be a universal regulator of flavonoid-pathway genes, as many pathway transcripts decrease in abundance when this gene is silenced.This report demonstrates that such systems-level approaches may be especially powerful when connected to an effective transient expression system, helping to provide rapid and strong evidence of gene function in key fruit-ripening processes.

View Article: PubMed Central - PubMed

Affiliation: Horticultural Sciences Department, University of Florida, Gainesville, FL 32611, USA.

No MeSH data available.


Simplified flavonoid biosynthetic pathway. PAL, Phenylalanine ammonia lyase; C4H, cinnamate-4-hydroxylase; 4CL, 4-coumaroyl:CoA-ligase; CHS, Chalcone synthase; CHI, Chalcone isomerase; F3H, Flavanone 3 hydrolase; F3’H, Flavanone 3’ hydrolase; FLS, Flavonol synthase; DFR, Dihydroflavanol reductase; LAR, Leucoanthocyanidin reductase; ANR, Anthocyanidin reductase; ANS, Anthocyanidin synthase; UFGT, UDP-Glucose flavonoid glycosyl transferase.
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Figure 1: Simplified flavonoid biosynthetic pathway. PAL, Phenylalanine ammonia lyase; C4H, cinnamate-4-hydroxylase; 4CL, 4-coumaroyl:CoA-ligase; CHS, Chalcone synthase; CHI, Chalcone isomerase; F3H, Flavanone 3 hydrolase; F3’H, Flavanone 3’ hydrolase; FLS, Flavonol synthase; DFR, Dihydroflavanol reductase; LAR, Leucoanthocyanidin reductase; ANR, Anthocyanidin reductase; ANS, Anthocyanidin synthase; UFGT, UDP-Glucose flavonoid glycosyl transferase.

Mentions: The phenylpropanoid pathway, and more specifically the flavonoid pathway, is a well-studied biosynthetic pathway that includes several enzymes that catalyse production of different families of molecules such as the flavonols, the condensed tannins and the anthocyanidins (Fig. 1). The core of this analysis measured transcripts from 15 genes known to be part of these pathways. These include the early and general phenylpropanoid genes such as the PHENYLALANINE AMMONIA LYASE (PAL) and some family-specific genes like the UDP-GLUCOSE FLAVONOID GLUCOSYL TRANSFERASE (UFGT) that leads to anthocyanin synthesis. Two isoforms of PAL and two of CHALCHONE SYNTHASE (CHS) were also included.


Identification of candidate flavonoid pathway genes using transcriptome correlation network analysis in ripe strawberry (Fragaria × ananassa) fruits.

Pillet J, Yu HW, Chambers AH, Whitaker VM, Folta KM - J. Exp. Bot. (2015)

Simplified flavonoid biosynthetic pathway. PAL, Phenylalanine ammonia lyase; C4H, cinnamate-4-hydroxylase; 4CL, 4-coumaroyl:CoA-ligase; CHS, Chalcone synthase; CHI, Chalcone isomerase; F3H, Flavanone 3 hydrolase; F3’H, Flavanone 3’ hydrolase; FLS, Flavonol synthase; DFR, Dihydroflavanol reductase; LAR, Leucoanthocyanidin reductase; ANR, Anthocyanidin reductase; ANS, Anthocyanidin synthase; UFGT, UDP-Glucose flavonoid glycosyl transferase.
© Copyright Policy - creative-commons
Related In: Results  -  Collection

License 1 - License 2
Show All Figures
getmorefigures.php?uid=PMC4507756&req=5

Figure 1: Simplified flavonoid biosynthetic pathway. PAL, Phenylalanine ammonia lyase; C4H, cinnamate-4-hydroxylase; 4CL, 4-coumaroyl:CoA-ligase; CHS, Chalcone synthase; CHI, Chalcone isomerase; F3H, Flavanone 3 hydrolase; F3’H, Flavanone 3’ hydrolase; FLS, Flavonol synthase; DFR, Dihydroflavanol reductase; LAR, Leucoanthocyanidin reductase; ANR, Anthocyanidin reductase; ANS, Anthocyanidin synthase; UFGT, UDP-Glucose flavonoid glycosyl transferase.
Mentions: The phenylpropanoid pathway, and more specifically the flavonoid pathway, is a well-studied biosynthetic pathway that includes several enzymes that catalyse production of different families of molecules such as the flavonols, the condensed tannins and the anthocyanidins (Fig. 1). The core of this analysis measured transcripts from 15 genes known to be part of these pathways. These include the early and general phenylpropanoid genes such as the PHENYLALANINE AMMONIA LYASE (PAL) and some family-specific genes like the UDP-GLUCOSE FLAVONOID GLUCOSYL TRANSFERASE (UFGT) that leads to anthocyanin synthesis. Two isoforms of PAL and two of CHALCHONE SYNTHASE (CHS) were also included.

Bottom Line: The results suggest that two of the newly-identified regulators likely contribute to discrete nodes of the flavonoid pathway.The third putative transcription factor appears to be a universal regulator of flavonoid-pathway genes, as many pathway transcripts decrease in abundance when this gene is silenced.This report demonstrates that such systems-level approaches may be especially powerful when connected to an effective transient expression system, helping to provide rapid and strong evidence of gene function in key fruit-ripening processes.

View Article: PubMed Central - PubMed

Affiliation: Horticultural Sciences Department, University of Florida, Gainesville, FL 32611, USA.

No MeSH data available.