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In vitro antioxidant, collagenase inhibition, and in vivo anti-wrinkle effects of combined formulation containing Punica granatum, Ginkgo biloba, Ficus carica, and Morus alba fruits extract.

Ghimeray AK, Jung US, Lee HY, Kim YH, Ryu EK, Chang MS - Clin Cosmet Investig Dermatol (2015)

Bottom Line: Facial wrinkle, especially the crow's feet region of eyes, was treated with topical formulated 2% cream for 56 days and compared with the placebo.In the in vivo study, treatment with 2% formulated cream for 56 days significantly reduced the percentage of wrinkle depth, length, and area with 11.5, 10.07, and 29.55, respectively.The combined formulation of fruit extracts showed excellent antioxidative and anti-collagenase activity as well as a significant effect on anti-wrinkle activity on human skin.

View Article: PubMed Central - PubMed

Affiliation: R&D Center, Natural Solution Co., Ltd, Gojan-dong, Namdong-gu, Incheon, Republic of Korea.

ABSTRACT

Background: In phytotherapy, the therapeutic potential is based on the combined action of different herbal drugs. Our objective was to evaluate the antioxidant, anti-collagenase (in vitro), and anti-wrinkle (in vivo) effect of combined formulation containing Ginkgo biloba, Punica granatum, Ficus carica, and Morus alba fruits extract.

Methods: Antioxidant evaluation was based on the scavenging activity of free radicals (1,1-diphenyl-2-picrylhydrazyl, H2O2, and O2 (-)) and the anti-collagenase activity was based on the reduction of collagenase enzyme in vitro. In an in vivo study, 21 female subjects were examined in a placebo-controlled trail. Facial wrinkle, especially the crow's feet region of eyes, was treated with topical formulated 2% cream for 56 days and compared with the placebo.

Results: In the in vitro study, the combination of fruits extract showed a higher antioxidant activity which was comparable with the positive standard (ascorbic acid, butylated hydroxyanisole, and Trolox). The data also showed a dose-dependent inhibition of collagenase. In the in vivo study, treatment with 2% formulated cream for 56 days significantly reduced the percentage of wrinkle depth, length, and area with 11.5, 10.07, and 29.55, respectively.

Conclusion: The combined formulation of fruit extracts showed excellent antioxidative and anti-collagenase activity as well as a significant effect on anti-wrinkle activity on human skin.

No MeSH data available.


Photograph showing the images of wrinkles used for assessment of wrinkle area, depth, and length in the crow’s feet region of subject’s eyes treated with 2% topically formulated fruits extract and placebo treated for 56 days. Clinical evaluations and measurements were performed on D0 (before treatment), D28, and D56.Abbreviation: D, day.
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f2-ccid-8-389: Photograph showing the images of wrinkles used for assessment of wrinkle area, depth, and length in the crow’s feet region of subject’s eyes treated with 2% topically formulated fruits extract and placebo treated for 56 days. Clinical evaluations and measurements were performed on D0 (before treatment), D28, and D56.Abbreviation: D, day.

Mentions: We examined the effect of 2% topical formulated fruit cream on the wrinkles of crow’s feet site of the eyes (Figure 2). The wrinkle area, depth, and length at the baseline were analyzed to identify the differences at sample treated sites. Treatment with 2% formulated cream for 28 days did not show any significant difference with the placebo. However, treatment for 56 days reduced significantly the percentage of wrinkle depth (Figure 3), length (Figure 4), and area (Figure 5) by 11.5%, 10.07%, and 29.55%, respectively, compared with the placebo. The dermatological scores of the sides treated by the extract containing cream decreased significantly (P<0.05) on 56 days with 1.5 fold lower than that of the placebo treatment (Figure 6).


In vitro antioxidant, collagenase inhibition, and in vivo anti-wrinkle effects of combined formulation containing Punica granatum, Ginkgo biloba, Ficus carica, and Morus alba fruits extract.

Ghimeray AK, Jung US, Lee HY, Kim YH, Ryu EK, Chang MS - Clin Cosmet Investig Dermatol (2015)

Photograph showing the images of wrinkles used for assessment of wrinkle area, depth, and length in the crow’s feet region of subject’s eyes treated with 2% topically formulated fruits extract and placebo treated for 56 days. Clinical evaluations and measurements were performed on D0 (before treatment), D28, and D56.Abbreviation: D, day.
© Copyright Policy
Related In: Results  -  Collection

License
Show All Figures
getmorefigures.php?uid=PMC4507742&req=5

f2-ccid-8-389: Photograph showing the images of wrinkles used for assessment of wrinkle area, depth, and length in the crow’s feet region of subject’s eyes treated with 2% topically formulated fruits extract and placebo treated for 56 days. Clinical evaluations and measurements were performed on D0 (before treatment), D28, and D56.Abbreviation: D, day.
Mentions: We examined the effect of 2% topical formulated fruit cream on the wrinkles of crow’s feet site of the eyes (Figure 2). The wrinkle area, depth, and length at the baseline were analyzed to identify the differences at sample treated sites. Treatment with 2% formulated cream for 28 days did not show any significant difference with the placebo. However, treatment for 56 days reduced significantly the percentage of wrinkle depth (Figure 3), length (Figure 4), and area (Figure 5) by 11.5%, 10.07%, and 29.55%, respectively, compared with the placebo. The dermatological scores of the sides treated by the extract containing cream decreased significantly (P<0.05) on 56 days with 1.5 fold lower than that of the placebo treatment (Figure 6).

Bottom Line: Facial wrinkle, especially the crow's feet region of eyes, was treated with topical formulated 2% cream for 56 days and compared with the placebo.In the in vivo study, treatment with 2% formulated cream for 56 days significantly reduced the percentage of wrinkle depth, length, and area with 11.5, 10.07, and 29.55, respectively.The combined formulation of fruit extracts showed excellent antioxidative and anti-collagenase activity as well as a significant effect on anti-wrinkle activity on human skin.

View Article: PubMed Central - PubMed

Affiliation: R&D Center, Natural Solution Co., Ltd, Gojan-dong, Namdong-gu, Incheon, Republic of Korea.

ABSTRACT

Background: In phytotherapy, the therapeutic potential is based on the combined action of different herbal drugs. Our objective was to evaluate the antioxidant, anti-collagenase (in vitro), and anti-wrinkle (in vivo) effect of combined formulation containing Ginkgo biloba, Punica granatum, Ficus carica, and Morus alba fruits extract.

Methods: Antioxidant evaluation was based on the scavenging activity of free radicals (1,1-diphenyl-2-picrylhydrazyl, H2O2, and O2 (-)) and the anti-collagenase activity was based on the reduction of collagenase enzyme in vitro. In an in vivo study, 21 female subjects were examined in a placebo-controlled trail. Facial wrinkle, especially the crow's feet region of eyes, was treated with topical formulated 2% cream for 56 days and compared with the placebo.

Results: In the in vitro study, the combination of fruits extract showed a higher antioxidant activity which was comparable with the positive standard (ascorbic acid, butylated hydroxyanisole, and Trolox). The data also showed a dose-dependent inhibition of collagenase. In the in vivo study, treatment with 2% formulated cream for 56 days significantly reduced the percentage of wrinkle depth, length, and area with 11.5, 10.07, and 29.55, respectively.

Conclusion: The combined formulation of fruit extracts showed excellent antioxidative and anti-collagenase activity as well as a significant effect on anti-wrinkle activity on human skin.

No MeSH data available.