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3D Laser Triangulation for Plant Phenotyping in Challenging Environments.

Kjaer KH, Ottosen CO - Sensors (Basel) (2015)

Bottom Line: This would not only broaden the application range of phenotyping in the plant research community, but also increase the ability for researchers to study plants in their natural environments.In the present paper, we evaluate a commercial 3D NIR-laser scanner (PlantEye, Phenospex B.V., Herleen, The Netherlands) to track daily changes in plant growth with high precision in challenging environments.Firstly, we demonstrate that the NIR laser beam of the scanner does not affect plant photosynthetic performance.

View Article: PubMed Central - PubMed

Affiliation: Department of Food Science, Aarhus University, Kirstinebjergvej 10, 5792 Aarslev, Denmark. katrine.kjaer@food.au.dk.

ABSTRACT
To increase the understanding of how the plant phenotype is formed by genotype and environmental interactions, simple and robust high-throughput plant phenotyping methods should be developed and considered. This would not only broaden the application range of phenotyping in the plant research community, but also increase the ability for researchers to study plants in their natural environments. By studying plants in their natural environment in high temporal resolution, more knowledge on how multiple stresses interact in defining the plant phenotype could lead to a better understanding of the interaction between plant responses and epigenetic regulation. In the present paper, we evaluate a commercial 3D NIR-laser scanner (PlantEye, Phenospex B.V., Herleen, The Netherlands) to track daily changes in plant growth with high precision in challenging environments. Firstly, we demonstrate that the NIR laser beam of the scanner does not affect plant photosynthetic performance. Secondly, we demonstrate that it is possible to estimate phenotypic variation amongst the growth pattern of ten genotypes of Brassica napus L. (rapeseed), using a simple linear correlation between scanned parameters and destructive growth measurements. Our results demonstrate the high potential of 3D laser triangulation for simple measurements of phenotypic variation in challenging environments and in a high temporal resolution.

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Related in: MedlinePlus

Correlation between the electron transport rate (ETR) and the photosynthetic active radiation (PAR) in rapeseed plants exposed to the near-infrared laser line from the 3D triangulation scanner or placed in a control treatment. Control plants (black dots) and scanned plants (white dots), n = 3.
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sensors-15-13533-f005: Correlation between the electron transport rate (ETR) and the photosynthetic active radiation (PAR) in rapeseed plants exposed to the near-infrared laser line from the 3D triangulation scanner or placed in a control treatment. Control plants (black dots) and scanned plants (white dots), n = 3.

Mentions: These measurements were made continuously every hour throughout the eight days, but the figure only shows mean values from the two time points 00:00 h (beginning of dark period) and 04:00 h (beginning of light period) for the eight consecutive days in order to avoid effects of differences in light irradiance between consecutive days. The electron transport rate (ETR) was linear related to PAR under the low light conditions in the two treatments showing that photochemistry and photosynthesis of the plants was unaffected by the NIR laser line (Figure 5).


3D Laser Triangulation for Plant Phenotyping in Challenging Environments.

Kjaer KH, Ottosen CO - Sensors (Basel) (2015)

Correlation between the electron transport rate (ETR) and the photosynthetic active radiation (PAR) in rapeseed plants exposed to the near-infrared laser line from the 3D triangulation scanner or placed in a control treatment. Control plants (black dots) and scanned plants (white dots), n = 3.
© Copyright Policy
Related In: Results  -  Collection

License
Show All Figures
getmorefigures.php?uid=PMC4507705&req=5

sensors-15-13533-f005: Correlation between the electron transport rate (ETR) and the photosynthetic active radiation (PAR) in rapeseed plants exposed to the near-infrared laser line from the 3D triangulation scanner or placed in a control treatment. Control plants (black dots) and scanned plants (white dots), n = 3.
Mentions: These measurements were made continuously every hour throughout the eight days, but the figure only shows mean values from the two time points 00:00 h (beginning of dark period) and 04:00 h (beginning of light period) for the eight consecutive days in order to avoid effects of differences in light irradiance between consecutive days. The electron transport rate (ETR) was linear related to PAR under the low light conditions in the two treatments showing that photochemistry and photosynthesis of the plants was unaffected by the NIR laser line (Figure 5).

Bottom Line: This would not only broaden the application range of phenotyping in the plant research community, but also increase the ability for researchers to study plants in their natural environments.In the present paper, we evaluate a commercial 3D NIR-laser scanner (PlantEye, Phenospex B.V., Herleen, The Netherlands) to track daily changes in plant growth with high precision in challenging environments.Firstly, we demonstrate that the NIR laser beam of the scanner does not affect plant photosynthetic performance.

View Article: PubMed Central - PubMed

Affiliation: Department of Food Science, Aarhus University, Kirstinebjergvej 10, 5792 Aarslev, Denmark. katrine.kjaer@food.au.dk.

ABSTRACT
To increase the understanding of how the plant phenotype is formed by genotype and environmental interactions, simple and robust high-throughput plant phenotyping methods should be developed and considered. This would not only broaden the application range of phenotyping in the plant research community, but also increase the ability for researchers to study plants in their natural environments. By studying plants in their natural environment in high temporal resolution, more knowledge on how multiple stresses interact in defining the plant phenotype could lead to a better understanding of the interaction between plant responses and epigenetic regulation. In the present paper, we evaluate a commercial 3D NIR-laser scanner (PlantEye, Phenospex B.V., Herleen, The Netherlands) to track daily changes in plant growth with high precision in challenging environments. Firstly, we demonstrate that the NIR laser beam of the scanner does not affect plant photosynthetic performance. Secondly, we demonstrate that it is possible to estimate phenotypic variation amongst the growth pattern of ten genotypes of Brassica napus L. (rapeseed), using a simple linear correlation between scanned parameters and destructive growth measurements. Our results demonstrate the high potential of 3D laser triangulation for simple measurements of phenotypic variation in challenging environments and in a high temporal resolution.

Show MeSH
Related in: MedlinePlus