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3D Laser Triangulation for Plant Phenotyping in Challenging Environments.

Kjaer KH, Ottosen CO - Sensors (Basel) (2015)

Bottom Line: This would not only broaden the application range of phenotyping in the plant research community, but also increase the ability for researchers to study plants in their natural environments.In the present paper, we evaluate a commercial 3D NIR-laser scanner (PlantEye, Phenospex B.V., Herleen, The Netherlands) to track daily changes in plant growth with high precision in challenging environments.Firstly, we demonstrate that the NIR laser beam of the scanner does not affect plant photosynthetic performance.

View Article: PubMed Central - PubMed

Affiliation: Department of Food Science, Aarhus University, Kirstinebjergvej 10, 5792 Aarslev, Denmark. katrine.kjaer@food.au.dk.

ABSTRACT
To increase the understanding of how the plant phenotype is formed by genotype and environmental interactions, simple and robust high-throughput plant phenotyping methods should be developed and considered. This would not only broaden the application range of phenotyping in the plant research community, but also increase the ability for researchers to study plants in their natural environments. By studying plants in their natural environment in high temporal resolution, more knowledge on how multiple stresses interact in defining the plant phenotype could lead to a better understanding of the interaction between plant responses and epigenetic regulation. In the present paper, we evaluate a commercial 3D NIR-laser scanner (PlantEye, Phenospex B.V., Herleen, The Netherlands) to track daily changes in plant growth with high precision in challenging environments. Firstly, we demonstrate that the NIR laser beam of the scanner does not affect plant photosynthetic performance. Secondly, we demonstrate that it is possible to estimate phenotypic variation amongst the growth pattern of ten genotypes of Brassica napus L. (rapeseed), using a simple linear correlation between scanned parameters and destructive growth measurements. Our results demonstrate the high potential of 3D laser triangulation for simple measurements of phenotypic variation in challenging environments and in a high temporal resolution.

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Related in: MedlinePlus

(A) Values of maximum photochemical efficiency of PSII (Fv/Fm) and (B) Quantum yield of PSII (F´q/F´m) in rapeseed plants exposed to the near-infrared laser line from the 3D triangulation scanner or placed in a control treatment. Control plants (grey bars) and scanned plants (white bars), n = 3 ± SE.
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sensors-15-13533-f004: (A) Values of maximum photochemical efficiency of PSII (Fv/Fm) and (B) Quantum yield of PSII (F´q/F´m) in rapeseed plants exposed to the near-infrared laser line from the 3D triangulation scanner or placed in a control treatment. Control plants (grey bars) and scanned plants (white bars), n = 3 ± SE.

Mentions: The diurnal measurements of PSII operating efficiency, (ΦPSII) and the electron transport rate (ETR) varied greatly in relation to the changing light conditions during the eight consecutive measurement days, and in relation to the actual light level at which the individual MONI-heads were positioned (results not shown). Furthermore, flashing with high light every 20 min during the night decreased the maximum photochemical yield of PSII (Fv/Fm), possibly because the leaves became less and less dark-adapted. However, Fv/Fm was always above 0.8, which is in the range of Fv/Fm values (0.79–0.84) shown not to affect plant yield [14]. As seen in Figure 4 there was no significant effect of the NIR laser line after 0, 20 and 40 min of exposure on the Fv/Fm or ΦPSII (F´q/F´m) values.


3D Laser Triangulation for Plant Phenotyping in Challenging Environments.

Kjaer KH, Ottosen CO - Sensors (Basel) (2015)

(A) Values of maximum photochemical efficiency of PSII (Fv/Fm) and (B) Quantum yield of PSII (F´q/F´m) in rapeseed plants exposed to the near-infrared laser line from the 3D triangulation scanner or placed in a control treatment. Control plants (grey bars) and scanned plants (white bars), n = 3 ± SE.
© Copyright Policy
Related In: Results  -  Collection

License
Show All Figures
getmorefigures.php?uid=PMC4507705&req=5

sensors-15-13533-f004: (A) Values of maximum photochemical efficiency of PSII (Fv/Fm) and (B) Quantum yield of PSII (F´q/F´m) in rapeseed plants exposed to the near-infrared laser line from the 3D triangulation scanner or placed in a control treatment. Control plants (grey bars) and scanned plants (white bars), n = 3 ± SE.
Mentions: The diurnal measurements of PSII operating efficiency, (ΦPSII) and the electron transport rate (ETR) varied greatly in relation to the changing light conditions during the eight consecutive measurement days, and in relation to the actual light level at which the individual MONI-heads were positioned (results not shown). Furthermore, flashing with high light every 20 min during the night decreased the maximum photochemical yield of PSII (Fv/Fm), possibly because the leaves became less and less dark-adapted. However, Fv/Fm was always above 0.8, which is in the range of Fv/Fm values (0.79–0.84) shown not to affect plant yield [14]. As seen in Figure 4 there was no significant effect of the NIR laser line after 0, 20 and 40 min of exposure on the Fv/Fm or ΦPSII (F´q/F´m) values.

Bottom Line: This would not only broaden the application range of phenotyping in the plant research community, but also increase the ability for researchers to study plants in their natural environments.In the present paper, we evaluate a commercial 3D NIR-laser scanner (PlantEye, Phenospex B.V., Herleen, The Netherlands) to track daily changes in plant growth with high precision in challenging environments.Firstly, we demonstrate that the NIR laser beam of the scanner does not affect plant photosynthetic performance.

View Article: PubMed Central - PubMed

Affiliation: Department of Food Science, Aarhus University, Kirstinebjergvej 10, 5792 Aarslev, Denmark. katrine.kjaer@food.au.dk.

ABSTRACT
To increase the understanding of how the plant phenotype is formed by genotype and environmental interactions, simple and robust high-throughput plant phenotyping methods should be developed and considered. This would not only broaden the application range of phenotyping in the plant research community, but also increase the ability for researchers to study plants in their natural environments. By studying plants in their natural environment in high temporal resolution, more knowledge on how multiple stresses interact in defining the plant phenotype could lead to a better understanding of the interaction between plant responses and epigenetic regulation. In the present paper, we evaluate a commercial 3D NIR-laser scanner (PlantEye, Phenospex B.V., Herleen, The Netherlands) to track daily changes in plant growth with high precision in challenging environments. Firstly, we demonstrate that the NIR laser beam of the scanner does not affect plant photosynthetic performance. Secondly, we demonstrate that it is possible to estimate phenotypic variation amongst the growth pattern of ten genotypes of Brassica napus L. (rapeseed), using a simple linear correlation between scanned parameters and destructive growth measurements. Our results demonstrate the high potential of 3D laser triangulation for simple measurements of phenotypic variation in challenging environments and in a high temporal resolution.

Show MeSH
Related in: MedlinePlus