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A Microfluidic Love-Wave Biosensing Device for PSA Detection Based on an Aptamer Beacon Probe.

Zhang F, Li S, Cao K, Wang P, Su Y, Zhu X, Wan Y - Sensors (Basel) (2015)

Bottom Line: DNA aptamer, or "artificial antibody", was used as the specific biorecognition probe for PSA capture.Furthermore, PDMS microfluidic channels were designed and fabricated to realize automated quantitative sample injection.The proposed sensor might be a promising alternative for point of care diagnostics.

View Article: PubMed Central - PubMed

Affiliation: School of Mechanical Engineering, Nanjing University of Science and Technology, Nanjing 210094, China. zhangfeng_njust@163.com.

ABSTRACT
A label-free and selective aptamer beacon-based Love-wave biosensing device was developed for prostate specific antigen (PSA) detection. The device consists of the following parts: LiTaO3 substrate with SiO2 film as wave guide layer, two set of inter-digital transducers (IDT), gold film for immobilization of the biorecongniton layer and a polydimethylsiloxane (PDMS) microfluidic channels. DNA aptamer, or "artificial antibody", was used as the specific biorecognition probe for PSA capture. Some nucleotides were added to the 3'-end of the aptamer to form a duplex with the 3'-end, turning the aptamer into an aptamer-beacon. Taking advantage of the selective target-induced assembly changes arising from the "aptamer beacon", highly selective and specific detection of PSA was achieved. Furthermore, PDMS microfluidic channels were designed and fabricated to realize automated quantitative sample injection. After optimization of the experimental conditions, the established device showed good performance for PSA detection between 10 ng/mL to 1 μg/mL, with a detection limit of 10 ng/mL. The proposed sensor might be a promising alternative for point of care diagnostics.

No MeSH data available.


Related in: MedlinePlus

Schematic of the Love-wave immunosensor: a two channel Love-wave sensor chip is used. One channel is for PSA and the other is for the non-specific protein BSA. For specific binding of the target PSA, an aptamer beacon probe was immobilized on the gold film.
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sensors-15-13839-f007: Schematic of the Love-wave immunosensor: a two channel Love-wave sensor chip is used. One channel is for PSA and the other is for the non-specific protein BSA. For specific binding of the target PSA, an aptamer beacon probe was immobilized on the gold film.

Mentions: The schematic of the Love-wave immunosensor is shown in Figure 7. As is known, the affinity force between a target and an aptamer is due to the specific secondary structure of the aptamer. In the presence of the target, the base paring of the aptamer creates a secondary structure such as a short helical arm to accommodate the target and form an “aptamer-target” complex. In this work, the capture probe has a “stem-loop” structure, which is stable and resist to non-specific binding. While the target protein is added, the “aptamer-target” affinity was higher than that of the aptamer beacon, leading to an adjustment of the probe to accommodate the target. It is reported that the target-induced conformational switching can efficiently reduce the non-specific binding, resulting in an improvement of selectivity [36,37,38].


A Microfluidic Love-Wave Biosensing Device for PSA Detection Based on an Aptamer Beacon Probe.

Zhang F, Li S, Cao K, Wang P, Su Y, Zhu X, Wan Y - Sensors (Basel) (2015)

Schematic of the Love-wave immunosensor: a two channel Love-wave sensor chip is used. One channel is for PSA and the other is for the non-specific protein BSA. For specific binding of the target PSA, an aptamer beacon probe was immobilized on the gold film.
© Copyright Policy
Related In: Results  -  Collection

License
Show All Figures
getmorefigures.php?uid=PMC4507688&req=5

sensors-15-13839-f007: Schematic of the Love-wave immunosensor: a two channel Love-wave sensor chip is used. One channel is for PSA and the other is for the non-specific protein BSA. For specific binding of the target PSA, an aptamer beacon probe was immobilized on the gold film.
Mentions: The schematic of the Love-wave immunosensor is shown in Figure 7. As is known, the affinity force between a target and an aptamer is due to the specific secondary structure of the aptamer. In the presence of the target, the base paring of the aptamer creates a secondary structure such as a short helical arm to accommodate the target and form an “aptamer-target” complex. In this work, the capture probe has a “stem-loop” structure, which is stable and resist to non-specific binding. While the target protein is added, the “aptamer-target” affinity was higher than that of the aptamer beacon, leading to an adjustment of the probe to accommodate the target. It is reported that the target-induced conformational switching can efficiently reduce the non-specific binding, resulting in an improvement of selectivity [36,37,38].

Bottom Line: DNA aptamer, or "artificial antibody", was used as the specific biorecognition probe for PSA capture.Furthermore, PDMS microfluidic channels were designed and fabricated to realize automated quantitative sample injection.The proposed sensor might be a promising alternative for point of care diagnostics.

View Article: PubMed Central - PubMed

Affiliation: School of Mechanical Engineering, Nanjing University of Science and Technology, Nanjing 210094, China. zhangfeng_njust@163.com.

ABSTRACT
A label-free and selective aptamer beacon-based Love-wave biosensing device was developed for prostate specific antigen (PSA) detection. The device consists of the following parts: LiTaO3 substrate with SiO2 film as wave guide layer, two set of inter-digital transducers (IDT), gold film for immobilization of the biorecongniton layer and a polydimethylsiloxane (PDMS) microfluidic channels. DNA aptamer, or "artificial antibody", was used as the specific biorecognition probe for PSA capture. Some nucleotides were added to the 3'-end of the aptamer to form a duplex with the 3'-end, turning the aptamer into an aptamer-beacon. Taking advantage of the selective target-induced assembly changes arising from the "aptamer beacon", highly selective and specific detection of PSA was achieved. Furthermore, PDMS microfluidic channels were designed and fabricated to realize automated quantitative sample injection. After optimization of the experimental conditions, the established device showed good performance for PSA detection between 10 ng/mL to 1 μg/mL, with a detection limit of 10 ng/mL. The proposed sensor might be a promising alternative for point of care diagnostics.

No MeSH data available.


Related in: MedlinePlus