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A Microfluidic Love-Wave Biosensing Device for PSA Detection Based on an Aptamer Beacon Probe.

Zhang F, Li S, Cao K, Wang P, Su Y, Zhu X, Wan Y - Sensors (Basel) (2015)

Bottom Line: DNA aptamer, or "artificial antibody", was used as the specific biorecognition probe for PSA capture.Furthermore, PDMS microfluidic channels were designed and fabricated to realize automated quantitative sample injection.The proposed sensor might be a promising alternative for point of care diagnostics.

View Article: PubMed Central - PubMed

Affiliation: School of Mechanical Engineering, Nanjing University of Science and Technology, Nanjing 210094, China. zhangfeng_njust@163.com.

ABSTRACT
A label-free and selective aptamer beacon-based Love-wave biosensing device was developed for prostate specific antigen (PSA) detection. The device consists of the following parts: LiTaO3 substrate with SiO2 film as wave guide layer, two set of inter-digital transducers (IDT), gold film for immobilization of the biorecongniton layer and a polydimethylsiloxane (PDMS) microfluidic channels. DNA aptamer, or "artificial antibody", was used as the specific biorecognition probe for PSA capture. Some nucleotides were added to the 3'-end of the aptamer to form a duplex with the 3'-end, turning the aptamer into an aptamer-beacon. Taking advantage of the selective target-induced assembly changes arising from the "aptamer beacon", highly selective and specific detection of PSA was achieved. Furthermore, PDMS microfluidic channels were designed and fabricated to realize automated quantitative sample injection. After optimization of the experimental conditions, the established device showed good performance for PSA detection between 10 ng/mL to 1 μg/mL, with a detection limit of 10 ng/mL. The proposed sensor might be a promising alternative for point of care diagnostics.

No MeSH data available.


Related in: MedlinePlus

Simulation of velocity distribution of fluid in the reaction chamber.
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sensors-15-13839-f006: Simulation of velocity distribution of fluid in the reaction chamber.

Mentions: A PDMS chip was designed to perform automated quantitative sample injections and biorecogniton reactions. The configuration of the reaction chamber was a key factor for reaction efficiency. In order to investigate the velocity distribution of fluid in the reaction chamber, Finite Element Analysis (FEA) was used to simulate the fluidic velocity. (Figure 6) The FEA parameters were as follows: conventional tetrahedral layout was used to mesh the field. The simulation fluid was set as water. The inlet fluid velocity was set to 0.05 m/s. The outlet pressure was set to 0 Pa and the other planes were set as walls. Then calculation was done using ANSYS 13.0 (ANSYS Co., Ltd., Pittsburgh, PA, USA) and a laminar flow model. As shown in Figure 6, the fluidic velocity in the reaction chamber is distributed evenly in the spindle type chamber, which offers mild reaction conditions for the biosensing assay.


A Microfluidic Love-Wave Biosensing Device for PSA Detection Based on an Aptamer Beacon Probe.

Zhang F, Li S, Cao K, Wang P, Su Y, Zhu X, Wan Y - Sensors (Basel) (2015)

Simulation of velocity distribution of fluid in the reaction chamber.
© Copyright Policy
Related In: Results  -  Collection

License
Show All Figures
getmorefigures.php?uid=PMC4507688&req=5

sensors-15-13839-f006: Simulation of velocity distribution of fluid in the reaction chamber.
Mentions: A PDMS chip was designed to perform automated quantitative sample injections and biorecogniton reactions. The configuration of the reaction chamber was a key factor for reaction efficiency. In order to investigate the velocity distribution of fluid in the reaction chamber, Finite Element Analysis (FEA) was used to simulate the fluidic velocity. (Figure 6) The FEA parameters were as follows: conventional tetrahedral layout was used to mesh the field. The simulation fluid was set as water. The inlet fluid velocity was set to 0.05 m/s. The outlet pressure was set to 0 Pa and the other planes were set as walls. Then calculation was done using ANSYS 13.0 (ANSYS Co., Ltd., Pittsburgh, PA, USA) and a laminar flow model. As shown in Figure 6, the fluidic velocity in the reaction chamber is distributed evenly in the spindle type chamber, which offers mild reaction conditions for the biosensing assay.

Bottom Line: DNA aptamer, or "artificial antibody", was used as the specific biorecognition probe for PSA capture.Furthermore, PDMS microfluidic channels were designed and fabricated to realize automated quantitative sample injection.The proposed sensor might be a promising alternative for point of care diagnostics.

View Article: PubMed Central - PubMed

Affiliation: School of Mechanical Engineering, Nanjing University of Science and Technology, Nanjing 210094, China. zhangfeng_njust@163.com.

ABSTRACT
A label-free and selective aptamer beacon-based Love-wave biosensing device was developed for prostate specific antigen (PSA) detection. The device consists of the following parts: LiTaO3 substrate with SiO2 film as wave guide layer, two set of inter-digital transducers (IDT), gold film for immobilization of the biorecongniton layer and a polydimethylsiloxane (PDMS) microfluidic channels. DNA aptamer, or "artificial antibody", was used as the specific biorecognition probe for PSA capture. Some nucleotides were added to the 3'-end of the aptamer to form a duplex with the 3'-end, turning the aptamer into an aptamer-beacon. Taking advantage of the selective target-induced assembly changes arising from the "aptamer beacon", highly selective and specific detection of PSA was achieved. Furthermore, PDMS microfluidic channels were designed and fabricated to realize automated quantitative sample injection. After optimization of the experimental conditions, the established device showed good performance for PSA detection between 10 ng/mL to 1 μg/mL, with a detection limit of 10 ng/mL. The proposed sensor might be a promising alternative for point of care diagnostics.

No MeSH data available.


Related in: MedlinePlus