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Application of Cavity Enhanced Absorption Spectroscopy to the Detection of Nitric Oxide, Carbonyl Sulphide, and Ethane--Breath Biomarkers of Serious Diseases.

Wojtas J - Sensors (Basel) (2015)

Bottom Line: The conducted experiments show that this type of diagnosis would significantly increase chances for effective therapy of some diseases.Additionally, it offers non-invasive and real time measurements, high sensitivity and selectivity as well as minimizing discomfort for patients.For that reason, such sensors can be used in screening for early detection of serious diseases.

View Article: PubMed Central - PubMed

Affiliation: Institute of Optoelectronics, Military University of Technology, 2 Kaliskiego Str., Warsaw 00-908, Poland. jwojtas@wat.edu.pl.

ABSTRACT
The paper presents one of the laser absorption spectroscopy techniques as an effective tool for sensitive analysis of trace gas species in human breath. Characterization of nitric oxide, carbonyl sulphide and ethane, and the selection of their absorption lines are described. Experiments with some biomarkers showed that detection of pathogenic changes at the molecular level is possible using this technique. Thanks to cavity enhanced spectroscopy application, detection limits at the ppb-level and short measurements time (<3 s) were achieved. Absorption lines of reference samples of the selected volatile biomarkers were probed using a distributed feedback quantum cascade laser and a tunable laser system consisting of an optical parametric oscillator and difference frequency generator. Setup using the first source provided a detection limit of 30 ppb for nitric oxide and 250 ppb for carbonyl sulphide. During experiments employing a second laser, detection limits of 0.9 ppb and 0.3 ppb were obtained for carbonyl sulphide and ethane, respectively. The conducted experiments show that this type of diagnosis would significantly increase chances for effective therapy of some diseases. Additionally, it offers non-invasive and real time measurements, high sensitivity and selectivity as well as minimizing discomfort for patients. For that reason, such sensors can be used in screening for early detection of serious diseases.

Show MeSH
(a) The absorption of NO at pressures of 1 atm (b) and of 0.1 atm for concentrations occurring in human breath: NO—35 ppb, CO2—5%, H2O after the drying procedure—279 ppm.
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sensors-15-14356-f001: (a) The absorption of NO at pressures of 1 atm (b) and of 0.1 atm for concentrations occurring in human breath: NO—35 ppb, CO2—5%, H2O after the drying procedure—279 ppm.

Mentions: NO has a strong absorption band near a wavelength of 5.26 µm. Figure 1 shows the absorption spectra of compounds which are the most significance interferents of the nitric oxide absorption spectrum.


Application of Cavity Enhanced Absorption Spectroscopy to the Detection of Nitric Oxide, Carbonyl Sulphide, and Ethane--Breath Biomarkers of Serious Diseases.

Wojtas J - Sensors (Basel) (2015)

(a) The absorption of NO at pressures of 1 atm (b) and of 0.1 atm for concentrations occurring in human breath: NO—35 ppb, CO2—5%, H2O after the drying procedure—279 ppm.
© Copyright Policy
Related In: Results  -  Collection

License
Show All Figures
getmorefigures.php?uid=PMC4507679&req=5

sensors-15-14356-f001: (a) The absorption of NO at pressures of 1 atm (b) and of 0.1 atm for concentrations occurring in human breath: NO—35 ppb, CO2—5%, H2O after the drying procedure—279 ppm.
Mentions: NO has a strong absorption band near a wavelength of 5.26 µm. Figure 1 shows the absorption spectra of compounds which are the most significance interferents of the nitric oxide absorption spectrum.

Bottom Line: The conducted experiments show that this type of diagnosis would significantly increase chances for effective therapy of some diseases.Additionally, it offers non-invasive and real time measurements, high sensitivity and selectivity as well as minimizing discomfort for patients.For that reason, such sensors can be used in screening for early detection of serious diseases.

View Article: PubMed Central - PubMed

Affiliation: Institute of Optoelectronics, Military University of Technology, 2 Kaliskiego Str., Warsaw 00-908, Poland. jwojtas@wat.edu.pl.

ABSTRACT
The paper presents one of the laser absorption spectroscopy techniques as an effective tool for sensitive analysis of trace gas species in human breath. Characterization of nitric oxide, carbonyl sulphide and ethane, and the selection of their absorption lines are described. Experiments with some biomarkers showed that detection of pathogenic changes at the molecular level is possible using this technique. Thanks to cavity enhanced spectroscopy application, detection limits at the ppb-level and short measurements time (<3 s) were achieved. Absorption lines of reference samples of the selected volatile biomarkers were probed using a distributed feedback quantum cascade laser and a tunable laser system consisting of an optical parametric oscillator and difference frequency generator. Setup using the first source provided a detection limit of 30 ppb for nitric oxide and 250 ppb for carbonyl sulphide. During experiments employing a second laser, detection limits of 0.9 ppb and 0.3 ppb were obtained for carbonyl sulphide and ethane, respectively. The conducted experiments show that this type of diagnosis would significantly increase chances for effective therapy of some diseases. Additionally, it offers non-invasive and real time measurements, high sensitivity and selectivity as well as minimizing discomfort for patients. For that reason, such sensors can be used in screening for early detection of serious diseases.

Show MeSH