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Magnetic-particle-sensing based diagnostic protocols and applications.

Takamura T, Ko PJ, Sharma J, Yukino R, Ishizawa S, Sandhu A - Sensors (Basel) (2015)

Bottom Line: First, the detection by Hall magnetic sensor with lock-in amplifier and alternative magnetic field is summarized.Then, our approach to detect sub-200 nm diameter target magnetic particles via relatively large micoro-sized "columnar particles" by optical microscopy is described.Subsequently, we summarize magnetic particle detection based on optical techniques; one method is based on the scattering of the magnetically-assembled nano-sized magnetic bead chain in rotating magnetic fields and the other one is based on the reflection of magnetic target particles and porous silicon.

View Article: PubMed Central - PubMed

Affiliation: Electronics-Inspired Interdisciplinary Research Institute (EIIRIS), Toyohashi University of Technology, 1-1 Hibarigaoka, Tempaku, Toyohashi, Aichi 441-8580, Japan. takamura@eiiris.tut.ac.jp.

ABSTRACT
Magnetic particle-labeled biomaterial detection has attracted much attention in recent years for a number of reasons; easy manipulation by external magnetic fields, easy functionalization of the surface, and large surface-to-volume ratio, to name but a few. In this review, we report on our recent investigations into the detection of nano-sized magnetic particles. First, the detection by Hall magnetic sensor with lock-in amplifier and alternative magnetic field is summarized. Then, our approach to detect sub-200 nm diameter target magnetic particles via relatively large micoro-sized "columnar particles" by optical microscopy is described. Subsequently, we summarize magnetic particle detection based on optical techniques; one method is based on the scattering of the magnetically-assembled nano-sized magnetic bead chain in rotating magnetic fields and the other one is based on the reflection of magnetic target particles and porous silicon. Finally, we report recent works with reference to more familiar industrial products (such as smartphone-based medical diagnosis systems and magnetic removal of unspecific-binded nano-sized particles, or "magnetic washing").

No MeSH data available.


Related in: MedlinePlus

A graph of transmitted light ration vs. the concentration of avidin added into biotinylated 200 nm-diameter particles’ solution. Adapted from [12].
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sensors-15-12983-f016: A graph of transmitted light ration vs. the concentration of avidin added into biotinylated 200 nm-diameter particles’ solution. Adapted from [12].

Mentions: Figure 16 shows the results for the detection of avidin using 200 nm diameter biotinylated nano-particles. The biotinylated particles were covered with 6 × 106 biotin groups/beads. The solution was irradiated with collimated white light and the 785 nm wavelength of transmitted light was monitored. The rotation frequency and external magnetic fields were 0.1 Hz and 0.95 kA·m−1, respectively. This procedure enabled the detection of avidin to a sensitivity of ~100 pM (6.7 ng/mL) over a dynamic range of at least three orders of magnitude. Notably, the rotating chains acted as both biomolecule probes and micromagnetic mixers, enabling detection of biomolecular recognition in less than 30 s.


Magnetic-particle-sensing based diagnostic protocols and applications.

Takamura T, Ko PJ, Sharma J, Yukino R, Ishizawa S, Sandhu A - Sensors (Basel) (2015)

A graph of transmitted light ration vs. the concentration of avidin added into biotinylated 200 nm-diameter particles’ solution. Adapted from [12].
© Copyright Policy
Related In: Results  -  Collection

License
Show All Figures
getmorefigures.php?uid=PMC4507658&req=5

sensors-15-12983-f016: A graph of transmitted light ration vs. the concentration of avidin added into biotinylated 200 nm-diameter particles’ solution. Adapted from [12].
Mentions: Figure 16 shows the results for the detection of avidin using 200 nm diameter biotinylated nano-particles. The biotinylated particles were covered with 6 × 106 biotin groups/beads. The solution was irradiated with collimated white light and the 785 nm wavelength of transmitted light was monitored. The rotation frequency and external magnetic fields were 0.1 Hz and 0.95 kA·m−1, respectively. This procedure enabled the detection of avidin to a sensitivity of ~100 pM (6.7 ng/mL) over a dynamic range of at least three orders of magnitude. Notably, the rotating chains acted as both biomolecule probes and micromagnetic mixers, enabling detection of biomolecular recognition in less than 30 s.

Bottom Line: First, the detection by Hall magnetic sensor with lock-in amplifier and alternative magnetic field is summarized.Then, our approach to detect sub-200 nm diameter target magnetic particles via relatively large micoro-sized "columnar particles" by optical microscopy is described.Subsequently, we summarize magnetic particle detection based on optical techniques; one method is based on the scattering of the magnetically-assembled nano-sized magnetic bead chain in rotating magnetic fields and the other one is based on the reflection of magnetic target particles and porous silicon.

View Article: PubMed Central - PubMed

Affiliation: Electronics-Inspired Interdisciplinary Research Institute (EIIRIS), Toyohashi University of Technology, 1-1 Hibarigaoka, Tempaku, Toyohashi, Aichi 441-8580, Japan. takamura@eiiris.tut.ac.jp.

ABSTRACT
Magnetic particle-labeled biomaterial detection has attracted much attention in recent years for a number of reasons; easy manipulation by external magnetic fields, easy functionalization of the surface, and large surface-to-volume ratio, to name but a few. In this review, we report on our recent investigations into the detection of nano-sized magnetic particles. First, the detection by Hall magnetic sensor with lock-in amplifier and alternative magnetic field is summarized. Then, our approach to detect sub-200 nm diameter target magnetic particles via relatively large micoro-sized "columnar particles" by optical microscopy is described. Subsequently, we summarize magnetic particle detection based on optical techniques; one method is based on the scattering of the magnetically-assembled nano-sized magnetic bead chain in rotating magnetic fields and the other one is based on the reflection of magnetic target particles and porous silicon. Finally, we report recent works with reference to more familiar industrial products (such as smartphone-based medical diagnosis systems and magnetic removal of unspecific-binded nano-sized particles, or "magnetic washing").

No MeSH data available.


Related in: MedlinePlus