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Impedance of the grape berry cuticle as a novel phenotypic trait to estimate resistance to Botrytis cinerea.

Herzog K, Wind R, Töpfer R - Sensors (Basel) (2015)

Bottom Line: Statistical experiments revealed highly significant correlations between relative impedance of CW and the resistance of grapevines to B. cinerea.An ordinal logistic regression analysis revealed a R2McFadden of 0.37 and confirmed the application of Zrel of CW for the prediction of bunch infection and in this way as novel phenotyping trait.Applying the I-sensor, a preliminary QTL region was identified indicating that the novel phenotypic trait is as well a valuable tool for genetic analyses.

View Article: PubMed Central - PubMed

Affiliation: Julius Kühn-Institut-Federal Research Centre of Cultivated Plants, Institute for Grapevine Breeding Geilweilerhof, Siebeldingen 76833, Germany. Katja.herzog@jki.bund.de.

ABSTRACT
Warm and moist weather conditions during berry ripening provoke Botrytis cinerea (B. cinerea) causing notable bunch rot on susceptible grapevines with the effect of reduced yield and wine quality. Resistance donors of genetic loci to increase B. cinerea resistance are widely unknown. Promising traits of resistance are represented by physical features like the thickness and permeability of the grape berry cuticle. Sensor-based phenotyping methods or genetic markers are rare for such traits. In the present study, the simple-to-handle I-sensor was developed. The sensor enables the fast and reliable measurement of electrical impedance of the grape berry cuticles and its epicuticular waxes (CW). Statistical experiments revealed highly significant correlations between relative impedance of CW and the resistance of grapevines to B. cinerea. Thus, the relative impedance Zrel of CW was identified as the most important phenotypic factor with regard to the prediction of grapevine resistance to B. cinerea. An ordinal logistic regression analysis revealed a R2McFadden of 0.37 and confirmed the application of Zrel of CW for the prediction of bunch infection and in this way as novel phenotyping trait. Applying the I-sensor, a preliminary QTL region was identified indicating that the novel phenotypic trait is as well a valuable tool for genetic analyses.

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Mean relative impedance Zrel of CW of three different groups: resistant, partially susceptible and susceptible grapevines. The Duncan multiple range test (level of significance α = 0.05) was carried out to distinguish the groups statistically based on the mean impedance. Standard deviation is shown in the graphs. Stars indicate significant differences in comparison to the resistant group. CW: intact cuticle with epicuticular waxes.
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sensors-15-12498-f006: Mean relative impedance Zrel of CW of three different groups: resistant, partially susceptible and susceptible grapevines. The Duncan multiple range test (level of significance α = 0.05) was carried out to distinguish the groups statistically based on the mean impedance. Standard deviation is shown in the graphs. Stars indicate significant differences in comparison to the resistant group. CW: intact cuticle with epicuticular waxes.

Mentions: To sum up, the Zrel of CW (Zrel of the cuticle with epicuticular waxes) showed the highest significant correlations. Thus, it was selected as the most promising indicator for the prediction of resistance of grape berries to B. cinerea using an ordinal regression analysis. However, the investigated genotypes were grouped on the basis of their corresponding B. cinerea susceptibility, i.e., a resistant, partially susceptible, and susceptible group, and with regard to the level of bunch compactness (medium and compact). The mean impedance Zrel of CW with standard deviation was calculated for each group (Figure 6).


Impedance of the grape berry cuticle as a novel phenotypic trait to estimate resistance to Botrytis cinerea.

Herzog K, Wind R, Töpfer R - Sensors (Basel) (2015)

Mean relative impedance Zrel of CW of three different groups: resistant, partially susceptible and susceptible grapevines. The Duncan multiple range test (level of significance α = 0.05) was carried out to distinguish the groups statistically based on the mean impedance. Standard deviation is shown in the graphs. Stars indicate significant differences in comparison to the resistant group. CW: intact cuticle with epicuticular waxes.
© Copyright Policy
Related In: Results  -  Collection

License
Show All Figures
getmorefigures.php?uid=PMC4507599&req=5

sensors-15-12498-f006: Mean relative impedance Zrel of CW of three different groups: resistant, partially susceptible and susceptible grapevines. The Duncan multiple range test (level of significance α = 0.05) was carried out to distinguish the groups statistically based on the mean impedance. Standard deviation is shown in the graphs. Stars indicate significant differences in comparison to the resistant group. CW: intact cuticle with epicuticular waxes.
Mentions: To sum up, the Zrel of CW (Zrel of the cuticle with epicuticular waxes) showed the highest significant correlations. Thus, it was selected as the most promising indicator for the prediction of resistance of grape berries to B. cinerea using an ordinal regression analysis. However, the investigated genotypes were grouped on the basis of their corresponding B. cinerea susceptibility, i.e., a resistant, partially susceptible, and susceptible group, and with regard to the level of bunch compactness (medium and compact). The mean impedance Zrel of CW with standard deviation was calculated for each group (Figure 6).

Bottom Line: Statistical experiments revealed highly significant correlations between relative impedance of CW and the resistance of grapevines to B. cinerea.An ordinal logistic regression analysis revealed a R2McFadden of 0.37 and confirmed the application of Zrel of CW for the prediction of bunch infection and in this way as novel phenotyping trait.Applying the I-sensor, a preliminary QTL region was identified indicating that the novel phenotypic trait is as well a valuable tool for genetic analyses.

View Article: PubMed Central - PubMed

Affiliation: Julius Kühn-Institut-Federal Research Centre of Cultivated Plants, Institute for Grapevine Breeding Geilweilerhof, Siebeldingen 76833, Germany. Katja.herzog@jki.bund.de.

ABSTRACT
Warm and moist weather conditions during berry ripening provoke Botrytis cinerea (B. cinerea) causing notable bunch rot on susceptible grapevines with the effect of reduced yield and wine quality. Resistance donors of genetic loci to increase B. cinerea resistance are widely unknown. Promising traits of resistance are represented by physical features like the thickness and permeability of the grape berry cuticle. Sensor-based phenotyping methods or genetic markers are rare for such traits. In the present study, the simple-to-handle I-sensor was developed. The sensor enables the fast and reliable measurement of electrical impedance of the grape berry cuticles and its epicuticular waxes (CW). Statistical experiments revealed highly significant correlations between relative impedance of CW and the resistance of grapevines to B. cinerea. Thus, the relative impedance Zrel of CW was identified as the most important phenotypic factor with regard to the prediction of grapevine resistance to B. cinerea. An ordinal logistic regression analysis revealed a R2McFadden of 0.37 and confirmed the application of Zrel of CW for the prediction of bunch infection and in this way as novel phenotyping trait. Applying the I-sensor, a preliminary QTL region was identified indicating that the novel phenotypic trait is as well a valuable tool for genetic analyses.

Show MeSH
Related in: MedlinePlus