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DNA-Based Nanobiosensors as an Emerging Platform for Detection of Disease.

Abu-Salah KM, Zourob MM, Mouffouk F, Alrokayan SA, Alaamery MA, Ansari AA - Sensors (Basel) (2015)

Bottom Line: Up-to-date results suggest that DNA-based nanobiosensors could be used effectively to provide simple, fast, cost-effective, sensitive and specific detection of some genetic, cancer, and infectious diseases.In addition, they could potentially be used as a platform to detect immunodeficiency, and neurological and other diseases.We discuss recent trends and applications of new strategies for DNA-based nanobiosensors, and emphasize the challenges in translating basic research to the clinical laboratory.

View Article: PubMed Central - PubMed

Affiliation: Research Chair for Medical Applications of Nanomaterials, King Saud University, PO Box 2454, Riyadh 11451, Saudi Arabia. k_abusalah@hotmail.com.

ABSTRACT
Detection of disease at an early stage is one of the biggest challenges in medicine. Different disciplines of science are working together in this regard. The goal of nanodiagnostics is to provide more accurate tools for earlier diagnosis, to reduce cost and to simplify healthcare delivery of effective and personalized medicine, especially with regard to chronic diseases (e.g., diabetes and cardiovascular diseases) that have high healthcare costs. Up-to-date results suggest that DNA-based nanobiosensors could be used effectively to provide simple, fast, cost-effective, sensitive and specific detection of some genetic, cancer, and infectious diseases. In addition, they could potentially be used as a platform to detect immunodeficiency, and neurological and other diseases. This review examines different types of DNA-based nanobiosensors, the basic principles upon which they are based and their advantages and potential in diagnosis of acute and chronic diseases. We discuss recent trends and applications of new strategies for DNA-based nanobiosensors, and emphasize the challenges in translating basic research to the clinical laboratory.

No MeSH data available.


Related in: MedlinePlus

Schematic presentation of immobilization of thiolated single-stranded probe DNA on the surface of ZnO for hybridization detection in double-stranded DNA (target DNA).
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sensors-15-14539-f004: Schematic presentation of immobilization of thiolated single-stranded probe DNA on the surface of ZnO for hybridization detection in double-stranded DNA (target DNA).

Mentions: Increased interest has grown recently in direct, label-free electrochemical detection systems. Such direct detection can be accomplished by monitoring electrical changes resulting from the hybridization event. In addition the safety is improved by eliminating the need of using an indicator [47,48,49]. Wang et al. [50] introduced the first indicator-free scheme. Ansari et al. [51] employed a probe specific to Neisseria gonorrhea, which is composed of 20-m thiolated oligonucleotide (th-ssDNA) immobilized onto a sol-gel derived nanostructured zinc oxide (ZnO) film, dip-coated onto a glass substrate of indium-tin-oxide (ITO) (Figure 4).


DNA-Based Nanobiosensors as an Emerging Platform for Detection of Disease.

Abu-Salah KM, Zourob MM, Mouffouk F, Alrokayan SA, Alaamery MA, Ansari AA - Sensors (Basel) (2015)

Schematic presentation of immobilization of thiolated single-stranded probe DNA on the surface of ZnO for hybridization detection in double-stranded DNA (target DNA).
© Copyright Policy
Related In: Results  -  Collection

License
Show All Figures
getmorefigures.php?uid=PMC4507582&req=5

sensors-15-14539-f004: Schematic presentation of immobilization of thiolated single-stranded probe DNA on the surface of ZnO for hybridization detection in double-stranded DNA (target DNA).
Mentions: Increased interest has grown recently in direct, label-free electrochemical detection systems. Such direct detection can be accomplished by monitoring electrical changes resulting from the hybridization event. In addition the safety is improved by eliminating the need of using an indicator [47,48,49]. Wang et al. [50] introduced the first indicator-free scheme. Ansari et al. [51] employed a probe specific to Neisseria gonorrhea, which is composed of 20-m thiolated oligonucleotide (th-ssDNA) immobilized onto a sol-gel derived nanostructured zinc oxide (ZnO) film, dip-coated onto a glass substrate of indium-tin-oxide (ITO) (Figure 4).

Bottom Line: Up-to-date results suggest that DNA-based nanobiosensors could be used effectively to provide simple, fast, cost-effective, sensitive and specific detection of some genetic, cancer, and infectious diseases.In addition, they could potentially be used as a platform to detect immunodeficiency, and neurological and other diseases.We discuss recent trends and applications of new strategies for DNA-based nanobiosensors, and emphasize the challenges in translating basic research to the clinical laboratory.

View Article: PubMed Central - PubMed

Affiliation: Research Chair for Medical Applications of Nanomaterials, King Saud University, PO Box 2454, Riyadh 11451, Saudi Arabia. k_abusalah@hotmail.com.

ABSTRACT
Detection of disease at an early stage is one of the biggest challenges in medicine. Different disciplines of science are working together in this regard. The goal of nanodiagnostics is to provide more accurate tools for earlier diagnosis, to reduce cost and to simplify healthcare delivery of effective and personalized medicine, especially with regard to chronic diseases (e.g., diabetes and cardiovascular diseases) that have high healthcare costs. Up-to-date results suggest that DNA-based nanobiosensors could be used effectively to provide simple, fast, cost-effective, sensitive and specific detection of some genetic, cancer, and infectious diseases. In addition, they could potentially be used as a platform to detect immunodeficiency, and neurological and other diseases. This review examines different types of DNA-based nanobiosensors, the basic principles upon which they are based and their advantages and potential in diagnosis of acute and chronic diseases. We discuss recent trends and applications of new strategies for DNA-based nanobiosensors, and emphasize the challenges in translating basic research to the clinical laboratory.

No MeSH data available.


Related in: MedlinePlus