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Enhanced production of prodigiosin by Serratia marcescens MO-1 using ram horn peptone.

Kurbanoglu EB, Ozdal M, Ozdal OG, Algur OF - Braz. J. Microbiol. (2015)

Bottom Line: The effect of RHP on the production of prodigiosin by S. marcescens MO-1 was investigated, and the results showed that RHP supplementation promoted the growth of MO-1 and increased the production of prodigiosin.As a result, RHP was demonstrated to be a suitable substrate for prodigiosin production.These results revealed that prodigiosin could be produced efficiently by S. marcescens using RHP.

View Article: PubMed Central - PubMed

Affiliation: Atatürk University, Department of Biology, Faculty of Science, Ataturk University, Erzurum, Turkey, Department of Biology, Faculty of Science, Ataturk University, Erzurum, Turkey.

ABSTRACT
This work addresses the production of prodigiosin from ram horn peptone (RHP) using MO-1, a local isolate in submerged culture. First, a novel gram-negative and rod-shaped bacterial strain, MO-1, was isolated from the body of the grasshopper (Poecilemon tauricola Ramme 1951), which was collected from pesticide-contaminated fields. Sequence analysis of 16S rDNA classified the microbe as Serratia marcescens. The substrate utilization potential (BIOLOG) and fatty acid methyl ester profile (FAME) of S. marcescens were also determined. The effect of RHP on the production of prodigiosin by S. marcescens MO-1 was investigated, and the results showed that RHP supplementation promoted the growth of MO-1 and increased the production of prodigiosin. A concentration of 0.4% (w/v) RHP resulted in the greatest yield of prodigiosin (277.74 mg/L) after 48 h when mannitol was used as the sole source of carbon. The pigment yield was also influenced by the types of carbon sources and peptones. As a result, RHP was demonstrated to be a suitable substrate for prodigiosin production. These results revealed that prodigiosin could be produced efficiently by S. marcescens using RHP.

No MeSH data available.


Related in: MedlinePlus

Comparison of the effect of RHP at various concentrations onprodigiosin production. Culture conditions: initial pH 7.0, 200 rpm, 28°C.
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f03: Comparison of the effect of RHP at various concentrations onprodigiosin production. Culture conditions: initial pH 7.0, 200 rpm, 28°C.

Mentions: The effects of RHP on the production of prodigiosin are presented in Figure 3. Prodigiosin is a known secondarymetabolite that does not have a role in growth, development and reproduction, asdo the primary metabolites, and is typically formed during the end or near thestationary phase of growth. Observed together, Figures 2 and 3 reveal thatthe production of prodigiosin increases linearly between 12 and 36 h.Additionally, the characteristics of the production curve are similar to theproduction pattern of secondary metabolites. According to Figures 2 and 3,biomass and prodigiosin production increase with increasing RHP concentrationsup to 0.4%; beyond 0.4% RHP concentrations (at 0.5 and 0.6% RHP) the productionsdecrease.


Enhanced production of prodigiosin by Serratia marcescens MO-1 using ram horn peptone.

Kurbanoglu EB, Ozdal M, Ozdal OG, Algur OF - Braz. J. Microbiol. (2015)

Comparison of the effect of RHP at various concentrations onprodigiosin production. Culture conditions: initial pH 7.0, 200 rpm, 28°C.
© Copyright Policy - open-access
Related In: Results  -  Collection

License
Show All Figures
getmorefigures.php?uid=PMC4507561&req=5

f03: Comparison of the effect of RHP at various concentrations onprodigiosin production. Culture conditions: initial pH 7.0, 200 rpm, 28°C.
Mentions: The effects of RHP on the production of prodigiosin are presented in Figure 3. Prodigiosin is a known secondarymetabolite that does not have a role in growth, development and reproduction, asdo the primary metabolites, and is typically formed during the end or near thestationary phase of growth. Observed together, Figures 2 and 3 reveal thatthe production of prodigiosin increases linearly between 12 and 36 h.Additionally, the characteristics of the production curve are similar to theproduction pattern of secondary metabolites. According to Figures 2 and 3,biomass and prodigiosin production increase with increasing RHP concentrationsup to 0.4%; beyond 0.4% RHP concentrations (at 0.5 and 0.6% RHP) the productionsdecrease.

Bottom Line: The effect of RHP on the production of prodigiosin by S. marcescens MO-1 was investigated, and the results showed that RHP supplementation promoted the growth of MO-1 and increased the production of prodigiosin.As a result, RHP was demonstrated to be a suitable substrate for prodigiosin production.These results revealed that prodigiosin could be produced efficiently by S. marcescens using RHP.

View Article: PubMed Central - PubMed

Affiliation: Atatürk University, Department of Biology, Faculty of Science, Ataturk University, Erzurum, Turkey, Department of Biology, Faculty of Science, Ataturk University, Erzurum, Turkey.

ABSTRACT
This work addresses the production of prodigiosin from ram horn peptone (RHP) using MO-1, a local isolate in submerged culture. First, a novel gram-negative and rod-shaped bacterial strain, MO-1, was isolated from the body of the grasshopper (Poecilemon tauricola Ramme 1951), which was collected from pesticide-contaminated fields. Sequence analysis of 16S rDNA classified the microbe as Serratia marcescens. The substrate utilization potential (BIOLOG) and fatty acid methyl ester profile (FAME) of S. marcescens were also determined. The effect of RHP on the production of prodigiosin by S. marcescens MO-1 was investigated, and the results showed that RHP supplementation promoted the growth of MO-1 and increased the production of prodigiosin. A concentration of 0.4% (w/v) RHP resulted in the greatest yield of prodigiosin (277.74 mg/L) after 48 h when mannitol was used as the sole source of carbon. The pigment yield was also influenced by the types of carbon sources and peptones. As a result, RHP was demonstrated to be a suitable substrate for prodigiosin production. These results revealed that prodigiosin could be produced efficiently by S. marcescens using RHP.

No MeSH data available.


Related in: MedlinePlus