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Characteristics of dr1790 disruptant and its functional analysis in Deinococcus radiodurans.

Cheng J, Wang H, Xu X, Wang L, Tian B, Hua Y - Braz. J. Microbiol. (2015)

Bottom Line: Transcript profiling by microarray and RT-PCR analyses of the dr1790 deletion mutant suggested that some genes that are involved in protein secretion and transport were strongly suppressed, while other genes that are involved in protein quality control, such as chaperones and proteases, were induced.In addition, the expression of genes with predicted functions that are involved in antioxidant systems, electron transport, and energy metabolism was significantly altered through the disruption of dr1790.Moreover, the results of proteomic analyses using 2-DE and MS also demonstrated that DR1790 contributed to D. radiodurans survival.

View Article: PubMed Central - PubMed

Affiliation: Institute of Horticulture, Zhejiang Academy of Agricultural Sciences, Hangzhou, China, Institute of Horticulture, Zhejiang Academy of Agricultural Sciences, Hangzhou, China.

ABSTRACT
Deinococcus radiodurans (DR) is an extremophile that is well known for its resistance to radiation, oxidants and desiccation. The gene dr1790 of D. radiodurans was predicted to encode a yellow-related protein. The primary objective of the present study was to characterize the biological function of the DR1790 protein, which is a member of the ancient yellow/major royal jelly (MRJ) protein family, in prokaryotes. Fluorescence labeling demonstrated that the yellow-related protein encoded by dr1790 is a membrane protein. The deletion of the dr1790 gene decreased the cell growth rate and sensitivity to hydrogen peroxide and radiation and increased the membrane permeability of D. radiodurans. Transcript profiling by microarray and RT-PCR analyses of the dr1790 deletion mutant suggested that some genes that are involved in protein secretion and transport were strongly suppressed, while other genes that are involved in protein quality control, such as chaperones and proteases, were induced. In addition, the expression of genes with predicted functions that are involved in antioxidant systems, electron transport, and energy metabolism was significantly altered through the disruption of dr1790. Moreover, the results of proteomic analyses using 2-DE and MS also demonstrated that DR1790 contributed to D. radiodurans survival. Taken together, these results indicate that the DR1790 protein from the ancient yellow protein family plays a pleiotropic role in the survival of prokaryotic cells and contributes to the extraordinary resistance of D. radiodurans against oxidative and radiation stresses.

No MeSH data available.


Related in: MedlinePlus

Growth of wild type D. radiodurans R1 compared withthe R1Δdr1790 mutant strain under normal conditions inthe lag (A) and log (B) phases. The error bars represent the standarddeviations from three experiments.
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f01: Growth of wild type D. radiodurans R1 compared withthe R1Δdr1790 mutant strain under normal conditions inthe lag (A) and log (B) phases. The error bars represent the standarddeviations from three experiments.

Mentions: We assayed the doubling time of cells in the lag and log phases. TheR1Δdr1790 mutant doubling time (2.1 ± 0.4 h) was notsignificantly slower than the doubling time (1.5 ± 0.4 h) of the wild type R1strain under aerobic conditions during the lag phase (p >0.05) (Figure 1A). However, theR1Δdr1790 mutant doubling time (3.1 ± 0.5 h) was slightlyslower than the doubling time (1.6 ± 0.2 h) of the wild type R1 strain underaerobic conditions during the log phase (p < 0.05) (Figure 1B).


Characteristics of dr1790 disruptant and its functional analysis in Deinococcus radiodurans.

Cheng J, Wang H, Xu X, Wang L, Tian B, Hua Y - Braz. J. Microbiol. (2015)

Growth of wild type D. radiodurans R1 compared withthe R1Δdr1790 mutant strain under normal conditions inthe lag (A) and log (B) phases. The error bars represent the standarddeviations from three experiments.
© Copyright Policy - open-access
Related In: Results  -  Collection

License
Show All Figures
getmorefigures.php?uid=PMC4507557&req=5

f01: Growth of wild type D. radiodurans R1 compared withthe R1Δdr1790 mutant strain under normal conditions inthe lag (A) and log (B) phases. The error bars represent the standarddeviations from three experiments.
Mentions: We assayed the doubling time of cells in the lag and log phases. TheR1Δdr1790 mutant doubling time (2.1 ± 0.4 h) was notsignificantly slower than the doubling time (1.5 ± 0.4 h) of the wild type R1strain under aerobic conditions during the lag phase (p >0.05) (Figure 1A). However, theR1Δdr1790 mutant doubling time (3.1 ± 0.5 h) was slightlyslower than the doubling time (1.6 ± 0.2 h) of the wild type R1 strain underaerobic conditions during the log phase (p < 0.05) (Figure 1B).

Bottom Line: Transcript profiling by microarray and RT-PCR analyses of the dr1790 deletion mutant suggested that some genes that are involved in protein secretion and transport were strongly suppressed, while other genes that are involved in protein quality control, such as chaperones and proteases, were induced.In addition, the expression of genes with predicted functions that are involved in antioxidant systems, electron transport, and energy metabolism was significantly altered through the disruption of dr1790.Moreover, the results of proteomic analyses using 2-DE and MS also demonstrated that DR1790 contributed to D. radiodurans survival.

View Article: PubMed Central - PubMed

Affiliation: Institute of Horticulture, Zhejiang Academy of Agricultural Sciences, Hangzhou, China, Institute of Horticulture, Zhejiang Academy of Agricultural Sciences, Hangzhou, China.

ABSTRACT
Deinococcus radiodurans (DR) is an extremophile that is well known for its resistance to radiation, oxidants and desiccation. The gene dr1790 of D. radiodurans was predicted to encode a yellow-related protein. The primary objective of the present study was to characterize the biological function of the DR1790 protein, which is a member of the ancient yellow/major royal jelly (MRJ) protein family, in prokaryotes. Fluorescence labeling demonstrated that the yellow-related protein encoded by dr1790 is a membrane protein. The deletion of the dr1790 gene decreased the cell growth rate and sensitivity to hydrogen peroxide and radiation and increased the membrane permeability of D. radiodurans. Transcript profiling by microarray and RT-PCR analyses of the dr1790 deletion mutant suggested that some genes that are involved in protein secretion and transport were strongly suppressed, while other genes that are involved in protein quality control, such as chaperones and proteases, were induced. In addition, the expression of genes with predicted functions that are involved in antioxidant systems, electron transport, and energy metabolism was significantly altered through the disruption of dr1790. Moreover, the results of proteomic analyses using 2-DE and MS also demonstrated that DR1790 contributed to D. radiodurans survival. Taken together, these results indicate that the DR1790 protein from the ancient yellow protein family plays a pleiotropic role in the survival of prokaryotic cells and contributes to the extraordinary resistance of D. radiodurans against oxidative and radiation stresses.

No MeSH data available.


Related in: MedlinePlus