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Borrelia burgdorferi sensu lato in humans in a rural area of Paraná State, Brazil.

Gonçalves DD, Moura RA, Nunes M, Carreira T, Vidotto O, Freitas JC, Vieira ML - Braz. J. Microbiol. (2015)

Bottom Line: This study describes the detection of Borrelia garinii and Borrelia burgdorferi sensu stricto (s.s.) in Brazilian individuals using PCR and DNA sequencing.Our results suggest that these species are emerging pathogens in this country, and additional studies are necessary to determine the epidemiological characteristics of this disease in Brazil.

View Article: PubMed Central - PubMed

Affiliation: Universidade Estadual de Londrina, Programa de Pós-gradução em Ciência Animal, Universidade Estadual de Londrina, Londrina, PR, Brasil, Programa de Pós-gradução em Ciência Animal, Universidade Estadual de Londrina, Londrina, PR, Brazil. ; Universidade Paranaense, Medicina Veterinária Preventiva, Universidade Paranaense, Umuarama, PR, Brasil, Medicina Veterinária Preventiva, Universidade Paranaense, Umuarama, PR, Brazil.

ABSTRACT
This study describes the detection of Borrelia garinii and Borrelia burgdorferi sensu stricto (s.s.) in Brazilian individuals using PCR and DNA sequencing. Our results suggest that these species are emerging pathogens in this country, and additional studies are necessary to determine the epidemiological characteristics of this disease in Brazil.

No MeSH data available.


Related in: MedlinePlus

BLAST sequence analysis. (A) Alignment comparison of sequences generated fromserum sample J-96 with the 5s(rrf)-23s (rrl) intergenic spacer region ofBorrelia burgdorferi B31 strain (AE000783.1); (B) Alignmentcomparison of sequences generated from serum sample J-70 with the 5S(rrf)-23S (rrl) intergenic spacer regionof Borrelia garinii (Pbi strain) (CP000013.1).
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f01: BLAST sequence analysis. (A) Alignment comparison of sequences generated fromserum sample J-96 with the 5s(rrf)-23s (rrl) intergenic spacer region ofBorrelia burgdorferi B31 strain (AE000783.1); (B) Alignmentcomparison of sequences generated from serum sample J-70 with the 5S(rrf)-23S (rrl) intergenic spacer regionof Borrelia garinii (Pbi strain) (CP000013.1).

Mentions: Of the 207 human serum samples analyzed, two (0.96%) showed positive nested-PCR resultsfor the B. burgdorferi s.l. complex with amplicon sizes of ~230 bp. TheBLAST analysis showed high sequence similarity (100%) with two different Borreliagenospecies. The nucleotide sequences obtained have been submitted to the GenBankdatabase under the accession numbers KF790698 and KF790699. One strain (J-70) wasidentified as B. garinii, and the other strain (J-96) was identified asBorrelia burgdorferi s.s. (Figure 1Aand 1B). Both samples were identified in males (15 and 72 years old,respectively) who worked with different animal species and performed various functions,such as assisting with births and slaughtering and castrating cattle. The analysis ofthe variables associated with the presence of Borrelia burgdorferi s.l.DNA is shown in Table 1.


Borrelia burgdorferi sensu lato in humans in a rural area of Paraná State, Brazil.

Gonçalves DD, Moura RA, Nunes M, Carreira T, Vidotto O, Freitas JC, Vieira ML - Braz. J. Microbiol. (2015)

BLAST sequence analysis. (A) Alignment comparison of sequences generated fromserum sample J-96 with the 5s(rrf)-23s (rrl) intergenic spacer region ofBorrelia burgdorferi B31 strain (AE000783.1); (B) Alignmentcomparison of sequences generated from serum sample J-70 with the 5S(rrf)-23S (rrl) intergenic spacer regionof Borrelia garinii (Pbi strain) (CP000013.1).
© Copyright Policy - open-access
Related In: Results  -  Collection

License
Show All Figures
getmorefigures.php?uid=PMC4507553&req=5

f01: BLAST sequence analysis. (A) Alignment comparison of sequences generated fromserum sample J-96 with the 5s(rrf)-23s (rrl) intergenic spacer region ofBorrelia burgdorferi B31 strain (AE000783.1); (B) Alignmentcomparison of sequences generated from serum sample J-70 with the 5S(rrf)-23S (rrl) intergenic spacer regionof Borrelia garinii (Pbi strain) (CP000013.1).
Mentions: Of the 207 human serum samples analyzed, two (0.96%) showed positive nested-PCR resultsfor the B. burgdorferi s.l. complex with amplicon sizes of ~230 bp. TheBLAST analysis showed high sequence similarity (100%) with two different Borreliagenospecies. The nucleotide sequences obtained have been submitted to the GenBankdatabase under the accession numbers KF790698 and KF790699. One strain (J-70) wasidentified as B. garinii, and the other strain (J-96) was identified asBorrelia burgdorferi s.s. (Figure 1Aand 1B). Both samples were identified in males (15 and 72 years old,respectively) who worked with different animal species and performed various functions,such as assisting with births and slaughtering and castrating cattle. The analysis ofthe variables associated with the presence of Borrelia burgdorferi s.l.DNA is shown in Table 1.

Bottom Line: This study describes the detection of Borrelia garinii and Borrelia burgdorferi sensu stricto (s.s.) in Brazilian individuals using PCR and DNA sequencing.Our results suggest that these species are emerging pathogens in this country, and additional studies are necessary to determine the epidemiological characteristics of this disease in Brazil.

View Article: PubMed Central - PubMed

Affiliation: Universidade Estadual de Londrina, Programa de Pós-gradução em Ciência Animal, Universidade Estadual de Londrina, Londrina, PR, Brasil, Programa de Pós-gradução em Ciência Animal, Universidade Estadual de Londrina, Londrina, PR, Brazil. ; Universidade Paranaense, Medicina Veterinária Preventiva, Universidade Paranaense, Umuarama, PR, Brasil, Medicina Veterinária Preventiva, Universidade Paranaense, Umuarama, PR, Brazil.

ABSTRACT
This study describes the detection of Borrelia garinii and Borrelia burgdorferi sensu stricto (s.s.) in Brazilian individuals using PCR and DNA sequencing. Our results suggest that these species are emerging pathogens in this country, and additional studies are necessary to determine the epidemiological characteristics of this disease in Brazil.

No MeSH data available.


Related in: MedlinePlus