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Molecular typing of Argentinian Mycobacterium avium subsp. paratuberculosis isolates by multiple-locus variable number-tandem repeat analysis.

Gioffré A, Correa Muñoz M, Alvarado Pinedo MF, Vaca R, Morsella C, Fiorentino MA, Paolicchi F, Ruybal P, Zumárraga M, Travería GE, Romano MI - Braz. J. Microbiol. (2015)

Bottom Line: INMV1 and INMV2 were the most frequent patterns, grouping 76.3% of the isolates.We were also able to demonstrate the coexistence of genotypes in herds and co-infection at the organism level.This study shows that all the patterns described are common to those described in Europe, suggesting an epidemiological link between the continents.

View Article: PubMed Central - PubMed

Affiliation: Instituto Nacional de Tecnología Agropecuaria, Instituto de Biotecnología, Centro de Investigación en Ciencias Veterinarias y Agronómicas, Instituto Nacional de Tecnología Agropecuaria, Buenos Aires, Argentina, Instituto de Biotecnología, Centro de Investigación en Ciencias Veterinarias y Agronómicas, Instituto Nacional de Tecnología Agropecuaria, Buenos Aires, Argentina.

ABSTRACT
Multiple-locus variable number-tandem repeat analysis (MLVA) of Mycobacterium avium subspecies paratuberculosis (MAP) isolates may contribute to the knowledge of strain diversity in Argentina. Although the diversity of MAP has been previously investigated in Argentina using IS900-RFLP, a small number of isolates were employed, and a low discriminative power was reached. The aim of the present study was to test the genetic diversity among MAP isolates using an MLVA approach based on 8 repetitive loci. We studied 97 isolates from cattle, goat and sheep and could describe 7 different patterns: INMV1, INMV2, INMV11, INMV13, INMV16, INMV33 and one incomplete pattern. INMV1 and INMV2 were the most frequent patterns, grouping 76.3% of the isolates. We were also able to demonstrate the coexistence of genotypes in herds and co-infection at the organism level. This study shows that all the patterns described are common to those described in Europe, suggesting an epidemiological link between the continents.

No MeSH data available.


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Mentions: We tested ninety-seven isolates from a MAP collection, as confirmed by theirmycobactin J dependence for growth, IS900-PCR (Englund et al., 1999) and F57-PCR(Vansnick et al.,2004). These isolates were classified by PCR and restrictionendonuclease analysis (PCR-REA) as "cattle type" (C type) (Marsh et al., 1999). The testedclinical isolates corresponded to cattle, (n = 78) goats (n = 16) and sheep (n =3) belonging to herds (n = 23) from thirteen geographic localities of the BuenosAires province (Fig. 1). The isolates wereobtained over the period from 2005 to 2010. A loopful of each isolate wassuspended in sterile distilled water, and cell lysis was performed by serialfreeze-boiling cycles. The samples were centrifuged (10,000 ×g, 5 min), and 2 μL of the supernatant was used as the templatein PCR reactions.


Molecular typing of Argentinian Mycobacterium avium subsp. paratuberculosis isolates by multiple-locus variable number-tandem repeat analysis.

Gioffré A, Correa Muñoz M, Alvarado Pinedo MF, Vaca R, Morsella C, Fiorentino MA, Paolicchi F, Ruybal P, Zumárraga M, Travería GE, Romano MI - Braz. J. Microbiol. (2015)

A geographic reference map providing basic geo-information of thestudy area.
© Copyright Policy - open-access
Related In: Results  -  Collection

License
Show All Figures
getmorefigures.php?uid=PMC4507551&req=5

f01: A geographic reference map providing basic geo-information of thestudy area.
Mentions: We tested ninety-seven isolates from a MAP collection, as confirmed by theirmycobactin J dependence for growth, IS900-PCR (Englund et al., 1999) and F57-PCR(Vansnick et al.,2004). These isolates were classified by PCR and restrictionendonuclease analysis (PCR-REA) as "cattle type" (C type) (Marsh et al., 1999). The testedclinical isolates corresponded to cattle, (n = 78) goats (n = 16) and sheep (n =3) belonging to herds (n = 23) from thirteen geographic localities of the BuenosAires province (Fig. 1). The isolates wereobtained over the period from 2005 to 2010. A loopful of each isolate wassuspended in sterile distilled water, and cell lysis was performed by serialfreeze-boiling cycles. The samples were centrifuged (10,000 ×g, 5 min), and 2 μL of the supernatant was used as the templatein PCR reactions.

Bottom Line: INMV1 and INMV2 were the most frequent patterns, grouping 76.3% of the isolates.We were also able to demonstrate the coexistence of genotypes in herds and co-infection at the organism level.This study shows that all the patterns described are common to those described in Europe, suggesting an epidemiological link between the continents.

View Article: PubMed Central - PubMed

Affiliation: Instituto Nacional de Tecnología Agropecuaria, Instituto de Biotecnología, Centro de Investigación en Ciencias Veterinarias y Agronómicas, Instituto Nacional de Tecnología Agropecuaria, Buenos Aires, Argentina, Instituto de Biotecnología, Centro de Investigación en Ciencias Veterinarias y Agronómicas, Instituto Nacional de Tecnología Agropecuaria, Buenos Aires, Argentina.

ABSTRACT
Multiple-locus variable number-tandem repeat analysis (MLVA) of Mycobacterium avium subspecies paratuberculosis (MAP) isolates may contribute to the knowledge of strain diversity in Argentina. Although the diversity of MAP has been previously investigated in Argentina using IS900-RFLP, a small number of isolates were employed, and a low discriminative power was reached. The aim of the present study was to test the genetic diversity among MAP isolates using an MLVA approach based on 8 repetitive loci. We studied 97 isolates from cattle, goat and sheep and could describe 7 different patterns: INMV1, INMV2, INMV11, INMV13, INMV16, INMV33 and one incomplete pattern. INMV1 and INMV2 were the most frequent patterns, grouping 76.3% of the isolates. We were also able to demonstrate the coexistence of genotypes in herds and co-infection at the organism level. This study shows that all the patterns described are common to those described in Europe, suggesting an epidemiological link between the continents.

No MeSH data available.


Related in: MedlinePlus