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Antimicrobial activity of the essential oil of Tetradenia riparia (Hochst.) Codd. (Lamiaceae) against cariogenic bacteria.

de Melo NI, de Carvalho CE, Fracarolli L, Cunha WR, Veneziani RC, Martins CH, Crotti AE - Braz. J. Microbiol. (2015)

Bottom Line: This paper aims to investigate the chemical composition and the antibacterial effects of the essential oil obtained from Tetradenia riparia leaves (TR-EO) grown in Southeastern Brazil against a representative panel of oral pathogens.TR-EO displayed MIC values between 31.2 and 500 μg/mL, with the lowest MIC value being obtained against Streptococcus mitis (31.2 μg/mL), S. mutans (62.5 μg/mL), S. sobrinus (31.2 μg/mL), and Lactobacillus casei (62.5 μg/mL).In time-kill experiments, TR-EO demonstrated bactericidal activity against S. mutans within the first 12 h, resulting in a curve profile similar to that of chlorhexidine.

View Article: PubMed Central - PubMed

Affiliation: Universidade de Franca, Núcleo de Pesquisas em Ciências Exatas e Tecnológicas, Universidade de Franca, Franca, SP, Brasil, Núcleo de Pesquisas em Ciências Exatas e Tecnológicas, Universidade de Franca, Franca, SP, Brazil.

ABSTRACT
In Brazilian folk medicine, Tetradenia riparia (Hochst.) Codd. (Lamiaceae) is used to treat toothaches and dental abscesses and diseases induced by worms, bacteria, or fungi. This paper aims to investigate the chemical composition and the antibacterial effects of the essential oil obtained from Tetradenia riparia leaves (TR-EO) grown in Southeastern Brazil against a representative panel of oral pathogens. We evaluated the antibacterial activity of TR-EO in terms of the minimal inhibitory concentration (MIC). We identified aromadendrene oxide (14.0%), (E,E)-farnesol (13.6%), dronabinol (12.5%), and fenchone (6.2%) as the major constituents of TR-EO. TR-EO displayed MIC values between 31.2 and 500 μg/mL, with the lowest MIC value being obtained against Streptococcus mitis (31.2 μg/mL), S. mutans (62.5 μg/mL), S. sobrinus (31.2 μg/mL), and Lactobacillus casei (62.5 μg/mL). In time-kill experiments, TR-EO demonstrated bactericidal activity against S. mutans within the first 12 h, resulting in a curve profile similar to that of chlorhexidine. These results revealed that the essential oil of Tetradenia riparia displays promising activity against most of the selected cariogenic bacteria, including Streptococcus mutans.

No MeSH data available.


Related in: MedlinePlus

Time-kill curve for the essential oil of T. riparia(TR-EO) against S. mutans (5 × 105 cfu/mL). CHD:chlorhexidine.
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f02: Time-kill curve for the essential oil of T. riparia(TR-EO) against S. mutans (5 × 105 cfu/mL). CHD:chlorhexidine.

Mentions: Analysis of Figure 2 revealed that (1) at itsMBC (62.5 μg/mL), TR-EO exhibited a bactericidal effect against S.mutans, the main cariogenic bacteria, within the first 12 h and that(2) its action became more pronounced after this period. We also constructedtime-kill curves using two and three times the MBC value (data not shown). However,we did not verify any significant differences between the periods, indicating thatno dose-dependent response effects existed for TR-EO in the assays conditions (p< 0.05). Moreover, the TR-EO and CHD time-kill curve profiles were verysimilar.


Antimicrobial activity of the essential oil of Tetradenia riparia (Hochst.) Codd. (Lamiaceae) against cariogenic bacteria.

de Melo NI, de Carvalho CE, Fracarolli L, Cunha WR, Veneziani RC, Martins CH, Crotti AE - Braz. J. Microbiol. (2015)

Time-kill curve for the essential oil of T. riparia(TR-EO) against S. mutans (5 × 105 cfu/mL). CHD:chlorhexidine.
© Copyright Policy - open-access
Related In: Results  -  Collection

License
Show All Figures
getmorefigures.php?uid=PMC4507545&req=5

f02: Time-kill curve for the essential oil of T. riparia(TR-EO) against S. mutans (5 × 105 cfu/mL). CHD:chlorhexidine.
Mentions: Analysis of Figure 2 revealed that (1) at itsMBC (62.5 μg/mL), TR-EO exhibited a bactericidal effect against S.mutans, the main cariogenic bacteria, within the first 12 h and that(2) its action became more pronounced after this period. We also constructedtime-kill curves using two and three times the MBC value (data not shown). However,we did not verify any significant differences between the periods, indicating thatno dose-dependent response effects existed for TR-EO in the assays conditions (p< 0.05). Moreover, the TR-EO and CHD time-kill curve profiles were verysimilar.

Bottom Line: This paper aims to investigate the chemical composition and the antibacterial effects of the essential oil obtained from Tetradenia riparia leaves (TR-EO) grown in Southeastern Brazil against a representative panel of oral pathogens.TR-EO displayed MIC values between 31.2 and 500 μg/mL, with the lowest MIC value being obtained against Streptococcus mitis (31.2 μg/mL), S. mutans (62.5 μg/mL), S. sobrinus (31.2 μg/mL), and Lactobacillus casei (62.5 μg/mL).In time-kill experiments, TR-EO demonstrated bactericidal activity against S. mutans within the first 12 h, resulting in a curve profile similar to that of chlorhexidine.

View Article: PubMed Central - PubMed

Affiliation: Universidade de Franca, Núcleo de Pesquisas em Ciências Exatas e Tecnológicas, Universidade de Franca, Franca, SP, Brasil, Núcleo de Pesquisas em Ciências Exatas e Tecnológicas, Universidade de Franca, Franca, SP, Brazil.

ABSTRACT
In Brazilian folk medicine, Tetradenia riparia (Hochst.) Codd. (Lamiaceae) is used to treat toothaches and dental abscesses and diseases induced by worms, bacteria, or fungi. This paper aims to investigate the chemical composition and the antibacterial effects of the essential oil obtained from Tetradenia riparia leaves (TR-EO) grown in Southeastern Brazil against a representative panel of oral pathogens. We evaluated the antibacterial activity of TR-EO in terms of the minimal inhibitory concentration (MIC). We identified aromadendrene oxide (14.0%), (E,E)-farnesol (13.6%), dronabinol (12.5%), and fenchone (6.2%) as the major constituents of TR-EO. TR-EO displayed MIC values between 31.2 and 500 μg/mL, with the lowest MIC value being obtained against Streptococcus mitis (31.2 μg/mL), S. mutans (62.5 μg/mL), S. sobrinus (31.2 μg/mL), and Lactobacillus casei (62.5 μg/mL). In time-kill experiments, TR-EO demonstrated bactericidal activity against S. mutans within the first 12 h, resulting in a curve profile similar to that of chlorhexidine. These results revealed that the essential oil of Tetradenia riparia displays promising activity against most of the selected cariogenic bacteria, including Streptococcus mutans.

No MeSH data available.


Related in: MedlinePlus