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Isolation and 2,4-D-degrading characteristics of Cupriavidus campinensis BJ71.

Han L, Zhao D, Li C - Braz. J. Microbiol. (2015)

Bottom Line: Up to 99.57% of the 2,4-D was degraded under optimal conditions after 6 days of incubation.In a biodegradation experiment, 87.13% and 42.53% of 2,4-D (initial concentration, 350 mg kg(-1)) was degraded in non-sterile and sterilized soil inoculated with BJ71, respectively, after 14 days.The 2,4-D degradation was more rapid in a soil microcosm including BJ71 than in a soil microcosm without BJ71.

View Article: PubMed Central - PubMed

Affiliation: The Key Laboratory of Plant Resources Conservation and Germplasm Innovation in Mountainous Region, Ministry of Education, Guiyang, China, The Key Laboratory of Plant Resources Conservation and Germplasm Innovation in Mountainous Region, Ministry of Education, Guiyang, China. ; Guizhou University, College of Life Sciences, Guizhou University, Guiyang, China, College of Life Sciences, Guizhou University, Guiyang, China. ; Guizhou University, Institute of Agro-Bioengineering, Guizhou University, Guiyang, China, Institute of Agro-Bioengineering, Guizhou University, Guiyang, China.

ABSTRACT
An indigenous bacterial strain capable of utilizing 2,4-dichlorophenoxyacetic acid as the sole carbon and energy source was isolated from a soil used for grown wheat with a long-term history of herbicide use in Beijing, China. The strain BJ71 was identified as Cupriavidus campinensis based on its 16S rRNA sequence analysis and morphological, physiological, and biochemical characteristics. The degradation characteristics of strain BJ71 were evaluated. The optimal conditions for 2,4-D degradation were as follows: pH 7.0, 30 °C, 3% (v/v) inoculum size, and an initial 2,4-D concentration of 350 mg L(-1). Up to 99.57% of the 2,4-D was degraded under optimal conditions after 6 days of incubation. Strain BJ71 was also able to degrade quizalofop and fluroxypyr. This is the first report of a 2,4-D-degrader containing tfdA gene that can utilize these two herbicides. In a biodegradation experiment, 87.13% and 42.53% of 2,4-D (initial concentration, 350 mg kg(-1)) was degraded in non-sterile and sterilized soil inoculated with BJ71, respectively, after 14 days. The 2,4-D degradation was more rapid in a soil microcosm including BJ71 than in a soil microcosm without BJ71. These results indicate that strain BJ71 is a potential candidate for the bioremediation of soil contaminated with the herbicide 2,4-D.

No MeSH data available.


Related in: MedlinePlus

Amount of 2,4-D remaining and cell growth during degradation of 2,4-Dby strain BJ71. Incubation conditions were as follows: pH 7.0, finalcell density at 420 nm adjusted to 0.1. Cells were inoculated into 150mL MSM (pH 7.0) containing 350 mg L−1 2,4-D in a 250-mLErlenmeyer flask at 30 °C and shaker at 150 rpm. Standard errors arewithin 5% of the mean.
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f05: Amount of 2,4-D remaining and cell growth during degradation of 2,4-Dby strain BJ71. Incubation conditions were as follows: pH 7.0, finalcell density at 420 nm adjusted to 0.1. Cells were inoculated into 150mL MSM (pH 7.0) containing 350 mg L−1 2,4-D in a 250-mLErlenmeyer flask at 30 °C and shaker at 150 rpm. Standard errors arewithin 5% of the mean.

Mentions: The optimal conditions for 2,4-D biodegradation by strain BJ71 were as follows:initial pH of 7.0, incubation temperature of 30 °C, inoculum size of 3.0% (v/v),and an initial 2,4-D concentration of 350 mg L−1. The typical growthof strain BJ71 was consistent with the degradation curves under optimalconditions (Figure 5). Up to 99.57% of the2,4-D was degraded by strain BJ71 under these optimum conditions after 6 days ofincubation.


Isolation and 2,4-D-degrading characteristics of Cupriavidus campinensis BJ71.

Han L, Zhao D, Li C - Braz. J. Microbiol. (2015)

Amount of 2,4-D remaining and cell growth during degradation of 2,4-Dby strain BJ71. Incubation conditions were as follows: pH 7.0, finalcell density at 420 nm adjusted to 0.1. Cells were inoculated into 150mL MSM (pH 7.0) containing 350 mg L−1 2,4-D in a 250-mLErlenmeyer flask at 30 °C and shaker at 150 rpm. Standard errors arewithin 5% of the mean.
© Copyright Policy - open-access
Related In: Results  -  Collection

License
Show All Figures
getmorefigures.php?uid=PMC4507535&req=5

f05: Amount of 2,4-D remaining and cell growth during degradation of 2,4-Dby strain BJ71. Incubation conditions were as follows: pH 7.0, finalcell density at 420 nm adjusted to 0.1. Cells were inoculated into 150mL MSM (pH 7.0) containing 350 mg L−1 2,4-D in a 250-mLErlenmeyer flask at 30 °C and shaker at 150 rpm. Standard errors arewithin 5% of the mean.
Mentions: The optimal conditions for 2,4-D biodegradation by strain BJ71 were as follows:initial pH of 7.0, incubation temperature of 30 °C, inoculum size of 3.0% (v/v),and an initial 2,4-D concentration of 350 mg L−1. The typical growthof strain BJ71 was consistent with the degradation curves under optimalconditions (Figure 5). Up to 99.57% of the2,4-D was degraded by strain BJ71 under these optimum conditions after 6 days ofincubation.

Bottom Line: Up to 99.57% of the 2,4-D was degraded under optimal conditions after 6 days of incubation.In a biodegradation experiment, 87.13% and 42.53% of 2,4-D (initial concentration, 350 mg kg(-1)) was degraded in non-sterile and sterilized soil inoculated with BJ71, respectively, after 14 days.The 2,4-D degradation was more rapid in a soil microcosm including BJ71 than in a soil microcosm without BJ71.

View Article: PubMed Central - PubMed

Affiliation: The Key Laboratory of Plant Resources Conservation and Germplasm Innovation in Mountainous Region, Ministry of Education, Guiyang, China, The Key Laboratory of Plant Resources Conservation and Germplasm Innovation in Mountainous Region, Ministry of Education, Guiyang, China. ; Guizhou University, College of Life Sciences, Guizhou University, Guiyang, China, College of Life Sciences, Guizhou University, Guiyang, China. ; Guizhou University, Institute of Agro-Bioengineering, Guizhou University, Guiyang, China, Institute of Agro-Bioengineering, Guizhou University, Guiyang, China.

ABSTRACT
An indigenous bacterial strain capable of utilizing 2,4-dichlorophenoxyacetic acid as the sole carbon and energy source was isolated from a soil used for grown wheat with a long-term history of herbicide use in Beijing, China. The strain BJ71 was identified as Cupriavidus campinensis based on its 16S rRNA sequence analysis and morphological, physiological, and biochemical characteristics. The degradation characteristics of strain BJ71 were evaluated. The optimal conditions for 2,4-D degradation were as follows: pH 7.0, 30 °C, 3% (v/v) inoculum size, and an initial 2,4-D concentration of 350 mg L(-1). Up to 99.57% of the 2,4-D was degraded under optimal conditions after 6 days of incubation. Strain BJ71 was also able to degrade quizalofop and fluroxypyr. This is the first report of a 2,4-D-degrader containing tfdA gene that can utilize these two herbicides. In a biodegradation experiment, 87.13% and 42.53% of 2,4-D (initial concentration, 350 mg kg(-1)) was degraded in non-sterile and sterilized soil inoculated with BJ71, respectively, after 14 days. The 2,4-D degradation was more rapid in a soil microcosm including BJ71 than in a soil microcosm without BJ71. These results indicate that strain BJ71 is a potential candidate for the bioremediation of soil contaminated with the herbicide 2,4-D.

No MeSH data available.


Related in: MedlinePlus