Limits...
Isolation of an aryloxyphenoxy propanoate (AOPP) herbicide-degrading strain Rhodococcus ruber JPL-2 and the cloning of a novel carboxylesterase gene (feh).

Hongming L, Xu L, Zhaojian G, Fan Y, Dingbin C, Jianchun Z, Jianhong X, Shunpeng L, Qing H - Braz. J. Microbiol. (2015)

Bottom Line: The strain JPL-2, capable of degrading fenoxaprop-P-ethyl (FE), was isolated from the soil of a wheat field and identified as Rhodococcus ruber.The initial step of the degradation pathway is to hydrolyze the carboxylic acid ester bond.Its molecular mass was approximately 39 kDa, and the catalytic efficiency of FeH followed the order of FE > quizalofop-P-ethyl > clodinafop-propargyl > cyhalofop-butyl > fluazifop-P-butyl > haloxyfop-P-methyl > diclofop-methy, which indicated that the chain length of the alcohol moiety strongly affected the hydrolysis activity of the FeH toward AOPP herbicides.

View Article: PubMed Central - PubMed

Affiliation: Nanjing Agricultural University, College of Life Science, Nanjing Agricultural University, Ministry of Agriculture, Nanjing, China, Key Laboratory of Agricultural Environmental Microbiology, College of Life Science, Nanjing Agricultural University, Ministry of Agriculture, Nanjing, China.

ABSTRACT
The strain JPL-2, capable of degrading fenoxaprop-P-ethyl (FE), was isolated from the soil of a wheat field and identified as Rhodococcus ruber. This strain could utilize FE as its sole carbon source and degrade 94.6% of 100 mg L(-1) FE in 54 h. Strain JPL-2 could also degrade other aryloxyphenoxy propanoate (AOPP) herbicides. The initial step of the degradation pathway is to hydrolyze the carboxylic acid ester bond. A novel esterase gene feh, encoding the FE-hydrolyzing carboxylesterase (FeH) responsible for this initial step, was cloned from strain JPL-2. Its molecular mass was approximately 39 kDa, and the catalytic efficiency of FeH followed the order of FE > quizalofop-P-ethyl > clodinafop-propargyl > cyhalofop-butyl > fluazifop-P-butyl > haloxyfop-P-methyl > diclofop-methy, which indicated that the chain length of the alcohol moiety strongly affected the hydrolysis activity of the FeH toward AOPP herbicides.

No MeSH data available.


Related in: MedlinePlus

HPLC-MS profile of the metabolite produced by FeH. A, B, HPLC spectraof FE and authentic FA. C, HPLC spectra of FE and its metabolite; D,negatively charged ions mass spectra for metabolite (2.22 min).
© Copyright Policy - open-access
Related In: Results  -  Collection

License
getmorefigures.php?uid=PMC4507534&req=5

f05: HPLC-MS profile of the metabolite produced by FeH. A, B, HPLC spectraof FE and authentic FA. C, HPLC spectra of FE and its metabolite; D,negatively charged ions mass spectra for metabolite (2.22 min).

Mentions: When FeH was added to the enzyme reaction mixture, a new metabolite with aretention time of 2.22 min in the HPLC spectrum was produced (Figures 5A and 5C). Its retention time was similar tothat of the authentic standards FA (Figure5B). In the MS spectrum, this metabolite showed a protonatedmolecular ion at m/z 331.9 (Figure 5D), which was consistent with the corresponding massspectrum of authentic standards FA. Based on these results, we confirmed thatFeH catalyzed the hydrolysis of the carboxylic acid ester bond in FE andproduced ethanol and FA.


Isolation of an aryloxyphenoxy propanoate (AOPP) herbicide-degrading strain Rhodococcus ruber JPL-2 and the cloning of a novel carboxylesterase gene (feh).

Hongming L, Xu L, Zhaojian G, Fan Y, Dingbin C, Jianchun Z, Jianhong X, Shunpeng L, Qing H - Braz. J. Microbiol. (2015)

HPLC-MS profile of the metabolite produced by FeH. A, B, HPLC spectraof FE and authentic FA. C, HPLC spectra of FE and its metabolite; D,negatively charged ions mass spectra for metabolite (2.22 min).
© Copyright Policy - open-access
Related In: Results  -  Collection

License
Show All Figures
getmorefigures.php?uid=PMC4507534&req=5

f05: HPLC-MS profile of the metabolite produced by FeH. A, B, HPLC spectraof FE and authentic FA. C, HPLC spectra of FE and its metabolite; D,negatively charged ions mass spectra for metabolite (2.22 min).
Mentions: When FeH was added to the enzyme reaction mixture, a new metabolite with aretention time of 2.22 min in the HPLC spectrum was produced (Figures 5A and 5C). Its retention time was similar tothat of the authentic standards FA (Figure5B). In the MS spectrum, this metabolite showed a protonatedmolecular ion at m/z 331.9 (Figure 5D), which was consistent with the corresponding massspectrum of authentic standards FA. Based on these results, we confirmed thatFeH catalyzed the hydrolysis of the carboxylic acid ester bond in FE andproduced ethanol and FA.

Bottom Line: The strain JPL-2, capable of degrading fenoxaprop-P-ethyl (FE), was isolated from the soil of a wheat field and identified as Rhodococcus ruber.The initial step of the degradation pathway is to hydrolyze the carboxylic acid ester bond.Its molecular mass was approximately 39 kDa, and the catalytic efficiency of FeH followed the order of FE > quizalofop-P-ethyl > clodinafop-propargyl > cyhalofop-butyl > fluazifop-P-butyl > haloxyfop-P-methyl > diclofop-methy, which indicated that the chain length of the alcohol moiety strongly affected the hydrolysis activity of the FeH toward AOPP herbicides.

View Article: PubMed Central - PubMed

Affiliation: Nanjing Agricultural University, College of Life Science, Nanjing Agricultural University, Ministry of Agriculture, Nanjing, China, Key Laboratory of Agricultural Environmental Microbiology, College of Life Science, Nanjing Agricultural University, Ministry of Agriculture, Nanjing, China.

ABSTRACT
The strain JPL-2, capable of degrading fenoxaprop-P-ethyl (FE), was isolated from the soil of a wheat field and identified as Rhodococcus ruber. This strain could utilize FE as its sole carbon source and degrade 94.6% of 100 mg L(-1) FE in 54 h. Strain JPL-2 could also degrade other aryloxyphenoxy propanoate (AOPP) herbicides. The initial step of the degradation pathway is to hydrolyze the carboxylic acid ester bond. A novel esterase gene feh, encoding the FE-hydrolyzing carboxylesterase (FeH) responsible for this initial step, was cloned from strain JPL-2. Its molecular mass was approximately 39 kDa, and the catalytic efficiency of FeH followed the order of FE > quizalofop-P-ethyl > clodinafop-propargyl > cyhalofop-butyl > fluazifop-P-butyl > haloxyfop-P-methyl > diclofop-methy, which indicated that the chain length of the alcohol moiety strongly affected the hydrolysis activity of the FeH toward AOPP herbicides.

No MeSH data available.


Related in: MedlinePlus