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Efficacy of fungal decolorization of a mixture of dyes belonging to different classes.

Przystas W, Zablocka-Godlewska E, Grabinska-Sota E - Braz. J. Microbiol. (2015)

Bottom Line: Shaking improved the efficacy and rate of the dye removal.The best results were reached for the MB strain, which removed 90% of the tested mixture under shaking conditions.The highest phytotoxicity decrease was noted in shaken samples where the elimination of dye mixture was the best.

View Article: PubMed Central - PubMed

Affiliation: Environmental Biotechnology Department, Silesian University of Technology, Gliwice, Poland, Environmental Biotechnology Department, Silesian University of Technology, Gliwice, Poland.

ABSTRACT
Dyes are the most difficult constituents to remove by conventional biological wastewater treatment. Colored wastewater is mainly eliminated by physical and chemical procedures, which are very expensive and have drawbacks. Therefore, the advantage of using biological processes, such as the biotransformation of dyes, is that they may lead to complete mineralization or formation of less toxic products. To prove the possibility of using fungal processes for decolorization and other applications, the analysis of the toxicity of the processes' products is required. The decolorization of the mixture of two dyes from different classes - triphenylmethane brilliant green and azo Evans blue (GB - total concentration 0.08 g/L, proportion 1:1 w/w) - by Pleurotus ostreatus (BWPH and MB), Gloeophyllum odoratum (DCa), RWP17 (Polyporus picipes) and Fusarium oxysporum (G1) was studied. Zootoxicity (Daphnia magna) and phytotoxicity (Lemna minor) changes were estimated at the end of the experiment. The mixture of dyes was significantly removed by all the strains that were tested with 96 h of experimental time. However, differences among strains from the same species (P. ostreatus) were noted. Shaking improved the efficacy and rate of the dye removal. In static samples, the removal of the mixture reached more than 51.9% and in shaken samples, more than 79.2%. Tests using the dead biomass of the fungi only adsorbed up to 37% of the dye mixture (strain BWPH), which suggests that the process with the living biomass involves the biotransformation of the dyes. The best results were reached for the MB strain, which removed 90% of the tested mixture under shaking conditions. Regardless of the efficacy of the dye removal, toxicity decreased from class V to class III in tests with D. magna. Tests with L. minor control samples were classified as class IV, and samples with certain strains were non-toxic. The highest phytotoxicity decrease was noted in shaken samples where the elimination of dye mixture was the best.

No MeSH data available.


Related in: MedlinePlus

UV-vis scan of the dyes and dye mixture used.
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f01: UV-vis scan of the dyes and dye mixture used.

Mentions: Water solutions of triphenylmethane dye brilliant green (POCh) and diazo dyeEvans blue (Sigma-Aldrich) were filter sterilized (Millipore cellulose filters Ø0.20 μm) and added to 5-day-old fungal cultures. The characteristics of bothdyes are presented in Table 1, and theUV-Vis scans are presented in Figure 1.The final concentration of the dye mixture was 0.08 g/L (0.04 g/L of brilliantgreen and 0.04 g/L of Evans blue). Control samples with the dyes were preparedin sterile medium used for microorganisms cultures and were shaken the same asthe inoculated samples. The influence of process conditions (shaking and staticcultures) on the decolorization effectiveness was evaluated. As suggestedpreviously by us (Przystas etal., 2012) and other authors (Wesenberg et al., 2003; Kwang-Soo, 2004), some strains remove dyesmore effectively under shaking conditions and some under static conditions.Cultures were incubated at 26 °C. Dead biomass was used to estimate biosorptionand was obtained by autoclaving (15 min., 121 °C, 1.5 atm) 5-day-old culturesprepared the same as samples with living biomass. All modifications, as well ascontrols, were performed four times.


Efficacy of fungal decolorization of a mixture of dyes belonging to different classes.

Przystas W, Zablocka-Godlewska E, Grabinska-Sota E - Braz. J. Microbiol. (2015)

UV-vis scan of the dyes and dye mixture used.
© Copyright Policy - open-access
Related In: Results  -  Collection

License
Show All Figures
getmorefigures.php?uid=PMC4507533&req=5

f01: UV-vis scan of the dyes and dye mixture used.
Mentions: Water solutions of triphenylmethane dye brilliant green (POCh) and diazo dyeEvans blue (Sigma-Aldrich) were filter sterilized (Millipore cellulose filters Ø0.20 μm) and added to 5-day-old fungal cultures. The characteristics of bothdyes are presented in Table 1, and theUV-Vis scans are presented in Figure 1.The final concentration of the dye mixture was 0.08 g/L (0.04 g/L of brilliantgreen and 0.04 g/L of Evans blue). Control samples with the dyes were preparedin sterile medium used for microorganisms cultures and were shaken the same asthe inoculated samples. The influence of process conditions (shaking and staticcultures) on the decolorization effectiveness was evaluated. As suggestedpreviously by us (Przystas etal., 2012) and other authors (Wesenberg et al., 2003; Kwang-Soo, 2004), some strains remove dyesmore effectively under shaking conditions and some under static conditions.Cultures were incubated at 26 °C. Dead biomass was used to estimate biosorptionand was obtained by autoclaving (15 min., 121 °C, 1.5 atm) 5-day-old culturesprepared the same as samples with living biomass. All modifications, as well ascontrols, were performed four times.

Bottom Line: Shaking improved the efficacy and rate of the dye removal.The best results were reached for the MB strain, which removed 90% of the tested mixture under shaking conditions.The highest phytotoxicity decrease was noted in shaken samples where the elimination of dye mixture was the best.

View Article: PubMed Central - PubMed

Affiliation: Environmental Biotechnology Department, Silesian University of Technology, Gliwice, Poland, Environmental Biotechnology Department, Silesian University of Technology, Gliwice, Poland.

ABSTRACT
Dyes are the most difficult constituents to remove by conventional biological wastewater treatment. Colored wastewater is mainly eliminated by physical and chemical procedures, which are very expensive and have drawbacks. Therefore, the advantage of using biological processes, such as the biotransformation of dyes, is that they may lead to complete mineralization or formation of less toxic products. To prove the possibility of using fungal processes for decolorization and other applications, the analysis of the toxicity of the processes' products is required. The decolorization of the mixture of two dyes from different classes - triphenylmethane brilliant green and azo Evans blue (GB - total concentration 0.08 g/L, proportion 1:1 w/w) - by Pleurotus ostreatus (BWPH and MB), Gloeophyllum odoratum (DCa), RWP17 (Polyporus picipes) and Fusarium oxysporum (G1) was studied. Zootoxicity (Daphnia magna) and phytotoxicity (Lemna minor) changes were estimated at the end of the experiment. The mixture of dyes was significantly removed by all the strains that were tested with 96 h of experimental time. However, differences among strains from the same species (P. ostreatus) were noted. Shaking improved the efficacy and rate of the dye removal. In static samples, the removal of the mixture reached more than 51.9% and in shaken samples, more than 79.2%. Tests using the dead biomass of the fungi only adsorbed up to 37% of the dye mixture (strain BWPH), which suggests that the process with the living biomass involves the biotransformation of the dyes. The best results were reached for the MB strain, which removed 90% of the tested mixture under shaking conditions. Regardless of the efficacy of the dye removal, toxicity decreased from class V to class III in tests with D. magna. Tests with L. minor control samples were classified as class IV, and samples with certain strains were non-toxic. The highest phytotoxicity decrease was noted in shaken samples where the elimination of dye mixture was the best.

No MeSH data available.


Related in: MedlinePlus