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Biodegradation of crude oil by individual bacterial strains and a mixed bacterial consortium.

Santisi S, Cappello S, Catalfamo M, Mancini G, Hassanshahian M, Genovese L, Giuliano L, Yakimov MM - Braz. J. Microbiol. (2015)

Bottom Line: All experiments were carried out in microcosms systems containing seawater (with and without addition of inorganic nutrients) and crude oil (unique carbon source).Measures of total and live bacterial abundance, Card-FISH and quali-, quantitative analysis of hydrocarbons (GC-FID) were carried out in order to elucidate the co-operative action of mixed microbial populations in the process of biodegradation of crude oil.All data obtained confirmed the fundamental role of bacteria belonging to Alcanivorax genus in the degradation of linear hydrocarbons in oil polluted environments.

View Article: PubMed Central - PubMed

Affiliation: Institute for Coastal Marine Environment, National Counsel of Research, Messina, Italy, Institute for Coastal Marine Environment, National Counsel of Research, Messina, Italy. ; Università degli Studi di Messina, School in Biology and Cellular Biotechnology, Faculty of Sciences, University of Messina, Messina, Italy, School in "Biology and Cellular Biotechnology", Faculty of Sciences, University of Messina, Messina, Italy.

ABSTRACT
Three bacterial isolates identified as Alcanivorax borkumensis SK2, Rhodococcus erythropolis HS4 and Pseudomonas stutzeri SDM, based on 16S rRNA gene sequences, were isolated from crude oil enrichments of natural seawater. Single strains and four bacterial consortia designed by mixing the single bacterial cultures respectively in the following ratios: (Alcanivorax: Pseudomonas, 1:1), (Alcanivorax: Rhodococcus, 1:1), (Pseudomonas: Rhodococcus, 1:1), and (Alcanivorax: Pseudomonas: Rhodococcus, 1:1:1), were analyzed in order to evaluate their oil degrading capability. All experiments were carried out in microcosms systems containing seawater (with and without addition of inorganic nutrients) and crude oil (unique carbon source). Measures of total and live bacterial abundance, Card-FISH and quali-, quantitative analysis of hydrocarbons (GC-FID) were carried out in order to elucidate the co-operative action of mixed microbial populations in the process of biodegradation of crude oil. All data obtained confirmed the fundamental role of bacteria belonging to Alcanivorax genus in the degradation of linear hydrocarbons in oil polluted environments.

No MeSH data available.


Related in: MedlinePlus

The non-metric multi-dimensional (nMDS) scaling plot related to thecapability biodegradation of n-alkanes of different bacteria andconsortia in study. A, Alcanivorax borkumensis SK2; P,Pseudomonas stuzteri SMD; R, Rhodococcuserythropolis HS4. Normal letter indicate the Natural SeaWater experimentation (SW), underlined letters indicate the Natural SeaWater + Inorganic Nutrients (SW + IN) experimentation.
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f07: The non-metric multi-dimensional (nMDS) scaling plot related to thecapability biodegradation of n-alkanes of different bacteria andconsortia in study. A, Alcanivorax borkumensis SK2; P,Pseudomonas stuzteri SMD; R, Rhodococcuserythropolis HS4. Normal letter indicate the Natural SeaWater experimentation (SW), underlined letters indicate the Natural SeaWater + Inorganic Nutrients (SW + IN) experimentation.

Mentions: For all strains, n-alkanes with a medium length (C12–C18)were degraded to a greater extent (rate of degradation > of ~ 70%) than andlong chains (C19–C30) because long-chain n-alkanes aresolid and their low solubility inhibits degradation by bacteria (Figs. 7 and 8).


Biodegradation of crude oil by individual bacterial strains and a mixed bacterial consortium.

Santisi S, Cappello S, Catalfamo M, Mancini G, Hassanshahian M, Genovese L, Giuliano L, Yakimov MM - Braz. J. Microbiol. (2015)

The non-metric multi-dimensional (nMDS) scaling plot related to thecapability biodegradation of n-alkanes of different bacteria andconsortia in study. A, Alcanivorax borkumensis SK2; P,Pseudomonas stuzteri SMD; R, Rhodococcuserythropolis HS4. Normal letter indicate the Natural SeaWater experimentation (SW), underlined letters indicate the Natural SeaWater + Inorganic Nutrients (SW + IN) experimentation.
© Copyright Policy - open-access
Related In: Results  -  Collection

License
Show All Figures
getmorefigures.php?uid=PMC4507529&req=5

f07: The non-metric multi-dimensional (nMDS) scaling plot related to thecapability biodegradation of n-alkanes of different bacteria andconsortia in study. A, Alcanivorax borkumensis SK2; P,Pseudomonas stuzteri SMD; R, Rhodococcuserythropolis HS4. Normal letter indicate the Natural SeaWater experimentation (SW), underlined letters indicate the Natural SeaWater + Inorganic Nutrients (SW + IN) experimentation.
Mentions: For all strains, n-alkanes with a medium length (C12–C18)were degraded to a greater extent (rate of degradation > of ~ 70%) than andlong chains (C19–C30) because long-chain n-alkanes aresolid and their low solubility inhibits degradation by bacteria (Figs. 7 and 8).

Bottom Line: All experiments were carried out in microcosms systems containing seawater (with and without addition of inorganic nutrients) and crude oil (unique carbon source).Measures of total and live bacterial abundance, Card-FISH and quali-, quantitative analysis of hydrocarbons (GC-FID) were carried out in order to elucidate the co-operative action of mixed microbial populations in the process of biodegradation of crude oil.All data obtained confirmed the fundamental role of bacteria belonging to Alcanivorax genus in the degradation of linear hydrocarbons in oil polluted environments.

View Article: PubMed Central - PubMed

Affiliation: Institute for Coastal Marine Environment, National Counsel of Research, Messina, Italy, Institute for Coastal Marine Environment, National Counsel of Research, Messina, Italy. ; Università degli Studi di Messina, School in Biology and Cellular Biotechnology, Faculty of Sciences, University of Messina, Messina, Italy, School in "Biology and Cellular Biotechnology", Faculty of Sciences, University of Messina, Messina, Italy.

ABSTRACT
Three bacterial isolates identified as Alcanivorax borkumensis SK2, Rhodococcus erythropolis HS4 and Pseudomonas stutzeri SDM, based on 16S rRNA gene sequences, were isolated from crude oil enrichments of natural seawater. Single strains and four bacterial consortia designed by mixing the single bacterial cultures respectively in the following ratios: (Alcanivorax: Pseudomonas, 1:1), (Alcanivorax: Rhodococcus, 1:1), (Pseudomonas: Rhodococcus, 1:1), and (Alcanivorax: Pseudomonas: Rhodococcus, 1:1:1), were analyzed in order to evaluate their oil degrading capability. All experiments were carried out in microcosms systems containing seawater (with and without addition of inorganic nutrients) and crude oil (unique carbon source). Measures of total and live bacterial abundance, Card-FISH and quali-, quantitative analysis of hydrocarbons (GC-FID) were carried out in order to elucidate the co-operative action of mixed microbial populations in the process of biodegradation of crude oil. All data obtained confirmed the fundamental role of bacteria belonging to Alcanivorax genus in the degradation of linear hydrocarbons in oil polluted environments.

No MeSH data available.


Related in: MedlinePlus