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Biodegradation of crude oil by individual bacterial strains and a mixed bacterial consortium.

Santisi S, Cappello S, Catalfamo M, Mancini G, Hassanshahian M, Genovese L, Giuliano L, Yakimov MM - Braz. J. Microbiol. (2015)

Bottom Line: All experiments were carried out in microcosms systems containing seawater (with and without addition of inorganic nutrients) and crude oil (unique carbon source).Measures of total and live bacterial abundance, Card-FISH and quali-, quantitative analysis of hydrocarbons (GC-FID) were carried out in order to elucidate the co-operative action of mixed microbial populations in the process of biodegradation of crude oil.All data obtained confirmed the fundamental role of bacteria belonging to Alcanivorax genus in the degradation of linear hydrocarbons in oil polluted environments.

View Article: PubMed Central - PubMed

Affiliation: Institute for Coastal Marine Environment, National Counsel of Research, Messina, Italy, Institute for Coastal Marine Environment, National Counsel of Research, Messina, Italy. ; Università degli Studi di Messina, School in Biology and Cellular Biotechnology, Faculty of Sciences, University of Messina, Messina, Italy, School in "Biology and Cellular Biotechnology", Faculty of Sciences, University of Messina, Messina, Italy.

ABSTRACT
Three bacterial isolates identified as Alcanivorax borkumensis SK2, Rhodococcus erythropolis HS4 and Pseudomonas stutzeri SDM, based on 16S rRNA gene sequences, were isolated from crude oil enrichments of natural seawater. Single strains and four bacterial consortia designed by mixing the single bacterial cultures respectively in the following ratios: (Alcanivorax: Pseudomonas, 1:1), (Alcanivorax: Rhodococcus, 1:1), (Pseudomonas: Rhodococcus, 1:1), and (Alcanivorax: Pseudomonas: Rhodococcus, 1:1:1), were analyzed in order to evaluate their oil degrading capability. All experiments were carried out in microcosms systems containing seawater (with and without addition of inorganic nutrients) and crude oil (unique carbon source). Measures of total and live bacterial abundance, Card-FISH and quali-, quantitative analysis of hydrocarbons (GC-FID) were carried out in order to elucidate the co-operative action of mixed microbial populations in the process of biodegradation of crude oil. All data obtained confirmed the fundamental role of bacteria belonging to Alcanivorax genus in the degradation of linear hydrocarbons in oil polluted environments.

No MeSH data available.


Phylogenetic tree based on 16S rRNA gene sequences for bacterialstrains (isolates isoSS-01, -02 and -03) used in this study. Percentagesof 100 bootstrap resampling that supported the branching orders in eachanalysis are shown above or near the relevant nodes. The tree was rootedand outgrouped (black arrow) by using the 16S rRNA sequences ofMethanococcus jannaschii (M59126). Evolutionarydistance is indicated by vertical lines; each scale bar lengthcorresponds to 0.05 fixed point mutations per sequence position.
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f01: Phylogenetic tree based on 16S rRNA gene sequences for bacterialstrains (isolates isoSS-01, -02 and -03) used in this study. Percentagesof 100 bootstrap resampling that supported the branching orders in eachanalysis are shown above or near the relevant nodes. The tree was rootedand outgrouped (black arrow) by using the 16S rRNA sequences ofMethanococcus jannaschii (M59126). Evolutionarydistance is indicated by vertical lines; each scale bar lengthcorresponds to 0.05 fixed point mutations per sequence position.

Mentions: Three bacterial strains named isoSS-01, corresponding to Alcanivoraxborkumensis strain SK2T (Genbank accession numberY12579; =DSM 11573T; 99%), isoSS-02 (Rhodococcuserythropolis HS4; Genebank accession numberAY168582; 99%) and isoSS-03 (Pseudomonas stutzeri SDM; Genebankaccession number DQ358054; 98%) were used in all the experiments (Fig. 1). Strain isoSS-01 belong to acollection of hydrocarbon-degrading bacteria hold at IAMC-Messina, strainsisoSS-2 and iso-SS03 were isolated from natural seawater from crude oilenrichments in previously research. All strains used in this study were isolatedfrom natural seawater from crude oil enrichments.


Biodegradation of crude oil by individual bacterial strains and a mixed bacterial consortium.

Santisi S, Cappello S, Catalfamo M, Mancini G, Hassanshahian M, Genovese L, Giuliano L, Yakimov MM - Braz. J. Microbiol. (2015)

Phylogenetic tree based on 16S rRNA gene sequences for bacterialstrains (isolates isoSS-01, -02 and -03) used in this study. Percentagesof 100 bootstrap resampling that supported the branching orders in eachanalysis are shown above or near the relevant nodes. The tree was rootedand outgrouped (black arrow) by using the 16S rRNA sequences ofMethanococcus jannaschii (M59126). Evolutionarydistance is indicated by vertical lines; each scale bar lengthcorresponds to 0.05 fixed point mutations per sequence position.
© Copyright Policy - open-access
Related In: Results  -  Collection

License
Show All Figures
getmorefigures.php?uid=PMC4507529&req=5

f01: Phylogenetic tree based on 16S rRNA gene sequences for bacterialstrains (isolates isoSS-01, -02 and -03) used in this study. Percentagesof 100 bootstrap resampling that supported the branching orders in eachanalysis are shown above or near the relevant nodes. The tree was rootedand outgrouped (black arrow) by using the 16S rRNA sequences ofMethanococcus jannaschii (M59126). Evolutionarydistance is indicated by vertical lines; each scale bar lengthcorresponds to 0.05 fixed point mutations per sequence position.
Mentions: Three bacterial strains named isoSS-01, corresponding to Alcanivoraxborkumensis strain SK2T (Genbank accession numberY12579; =DSM 11573T; 99%), isoSS-02 (Rhodococcuserythropolis HS4; Genebank accession numberAY168582; 99%) and isoSS-03 (Pseudomonas stutzeri SDM; Genebankaccession number DQ358054; 98%) were used in all the experiments (Fig. 1). Strain isoSS-01 belong to acollection of hydrocarbon-degrading bacteria hold at IAMC-Messina, strainsisoSS-2 and iso-SS03 were isolated from natural seawater from crude oilenrichments in previously research. All strains used in this study were isolatedfrom natural seawater from crude oil enrichments.

Bottom Line: All experiments were carried out in microcosms systems containing seawater (with and without addition of inorganic nutrients) and crude oil (unique carbon source).Measures of total and live bacterial abundance, Card-FISH and quali-, quantitative analysis of hydrocarbons (GC-FID) were carried out in order to elucidate the co-operative action of mixed microbial populations in the process of biodegradation of crude oil.All data obtained confirmed the fundamental role of bacteria belonging to Alcanivorax genus in the degradation of linear hydrocarbons in oil polluted environments.

View Article: PubMed Central - PubMed

Affiliation: Institute for Coastal Marine Environment, National Counsel of Research, Messina, Italy, Institute for Coastal Marine Environment, National Counsel of Research, Messina, Italy. ; Università degli Studi di Messina, School in Biology and Cellular Biotechnology, Faculty of Sciences, University of Messina, Messina, Italy, School in "Biology and Cellular Biotechnology", Faculty of Sciences, University of Messina, Messina, Italy.

ABSTRACT
Three bacterial isolates identified as Alcanivorax borkumensis SK2, Rhodococcus erythropolis HS4 and Pseudomonas stutzeri SDM, based on 16S rRNA gene sequences, were isolated from crude oil enrichments of natural seawater. Single strains and four bacterial consortia designed by mixing the single bacterial cultures respectively in the following ratios: (Alcanivorax: Pseudomonas, 1:1), (Alcanivorax: Rhodococcus, 1:1), (Pseudomonas: Rhodococcus, 1:1), and (Alcanivorax: Pseudomonas: Rhodococcus, 1:1:1), were analyzed in order to evaluate their oil degrading capability. All experiments were carried out in microcosms systems containing seawater (with and without addition of inorganic nutrients) and crude oil (unique carbon source). Measures of total and live bacterial abundance, Card-FISH and quali-, quantitative analysis of hydrocarbons (GC-FID) were carried out in order to elucidate the co-operative action of mixed microbial populations in the process of biodegradation of crude oil. All data obtained confirmed the fundamental role of bacteria belonging to Alcanivorax genus in the degradation of linear hydrocarbons in oil polluted environments.

No MeSH data available.