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Initial pH of medium affects organic acids production but do not affect phosphate solubilization.

Marra LM, de Oliveira-Longatti SM, Soares CR, de Lima JM, Olivares FL, Moreira FM - Braz. J. Microbiol. (2015)

Bottom Line: The strains UFLA03-08, UFLA03-09, UFLA03-10 and UFLA03-106 solubilized Ca3(PO4)2 in liquid medium regardless of the initial pH, although without a significant difference between the treatments.The production of organic acids by these strains was assessed for all of the initial pH values investigated, and differences between the treatments were observed.There was no correlation between phosphorus solubilized from Ca3(PO4)2 in NBRIP liquid medium and the concentration of total organic acids at the different initial pH values.

View Article: PubMed Central - PubMed

Affiliation: Universidade Federal de Lavras, Programa de Pós-Graduação em Ciência do Solo, Departamento de Ciência do Solo, Universidade Federal de Lavras, Lavras, MG, Brasil, Programa de Pós-Graduação em Ciência do Solo, Departamento de Ciência do Solo, Universidade Federal de Lavras, Lavras, MG, Brazil.

ABSTRACT
The pH of the culture medium directly influences the growth of microorganisms and the chemical processes that they perform. The aim of this study was to assess the influence of the initial pH of the culture medium on the production of 11 low-molecular-weight organic acids and on the solubilization of calcium phosphate by bacteria in growth medium (NBRIP). The following strains isolated from cowpea nodules were studied: UFLA03-08 (Rhizobium tropici), UFLA03-09 (Acinetobacter sp.), UFLA03-10 (Paenibacillus kribbensis), UFLA03-106 (Paenibacillus kribbensis) and UFLA03-116 (Paenibacillus sp.). The strains UFLA03-08, UFLA03-09, UFLA03-10 and UFLA03-106 solubilized Ca3(PO4)2 in liquid medium regardless of the initial pH, although without a significant difference between the treatments. The production of organic acids by these strains was assessed for all of the initial pH values investigated, and differences between the treatments were observed. Strains UFLA03-09 and UFLA03-10 produced the same acids at different initial pH values in the culture medium. There was no correlation between phosphorus solubilized from Ca3(PO4)2 in NBRIP liquid medium and the concentration of total organic acids at the different initial pH values. Therefore, the initial pH of the culture medium influences the production of organic acids by the strains UFLA03-08, UFLA03-09, UFLA03-10 and UFLA03-106 but it does not affect calcium phosphate solubilization.

No MeSH data available.


Identification and quantification of organic acids produced in liquidNBRIP culture medium at different initial pH values, inoculated withdifferent bacterial strains. Numbers in parentheses correspond to acidconcentration (mmol L−1). (Chromatograms - A: control/pH 5.0; B:control/pH 6.0; C: control/pH 7.0; D: UFLA 03-116/pH 5.0; E: UFLA 03-116/pH6.0; F: UFLA 03-116/pH 7.0; G: UFLA 03-08/pH 5.0; H: UFLA 03-08/pH 6.0; I:UFLA 03-08/pH 7.0; J: UFLA 03-09/pH 5.0 K: UFLA 03-09/pH 6.0; L: UFLA03-09/pH 7.0; M: UFLA 03-10/pH 5.0; N: UFLA 03-10/pH 6.0; O: UFLA 03-10/pH7.0; P: UFLA 03-106/pH 5.0; Q: UFLA 03-106/pH 6.0; R: UFLA 03-106/pH7.0).
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f04: Identification and quantification of organic acids produced in liquidNBRIP culture medium at different initial pH values, inoculated withdifferent bacterial strains. Numbers in parentheses correspond to acidconcentration (mmol L−1). (Chromatograms - A: control/pH 5.0; B:control/pH 6.0; C: control/pH 7.0; D: UFLA 03-116/pH 5.0; E: UFLA 03-116/pH6.0; F: UFLA 03-116/pH 7.0; G: UFLA 03-08/pH 5.0; H: UFLA 03-08/pH 6.0; I:UFLA 03-08/pH 7.0; J: UFLA 03-09/pH 5.0 K: UFLA 03-09/pH 6.0; L: UFLA03-09/pH 7.0; M: UFLA 03-10/pH 5.0; N: UFLA 03-10/pH 6.0; O: UFLA 03-10/pH7.0; P: UFLA 03-106/pH 5.0; Q: UFLA 03-106/pH 6.0; R: UFLA 03-106/pH7.0).

Mentions: With respect to the identification, for the strain UFLA 03-08 (Rhizobiumtropici), the highest total acid concentration (malic acid, 18.90 mmolL−1) was found at an initial pH of 7.0; for this strain, acidproduction varied with the initial pH of the culture medium. Organic acidsidentified at pH 5.0 and 6.0 were respectively: 2-ketogluconic (1.28 mmolL−1) and lactic/succinic acid (0.37 mmol L−1). Other peakswere not identified in all pH values (Figure 4- Chromatograms G, H and I).


Initial pH of medium affects organic acids production but do not affect phosphate solubilization.

Marra LM, de Oliveira-Longatti SM, Soares CR, de Lima JM, Olivares FL, Moreira FM - Braz. J. Microbiol. (2015)

Identification and quantification of organic acids produced in liquidNBRIP culture medium at different initial pH values, inoculated withdifferent bacterial strains. Numbers in parentheses correspond to acidconcentration (mmol L−1). (Chromatograms - A: control/pH 5.0; B:control/pH 6.0; C: control/pH 7.0; D: UFLA 03-116/pH 5.0; E: UFLA 03-116/pH6.0; F: UFLA 03-116/pH 7.0; G: UFLA 03-08/pH 5.0; H: UFLA 03-08/pH 6.0; I:UFLA 03-08/pH 7.0; J: UFLA 03-09/pH 5.0 K: UFLA 03-09/pH 6.0; L: UFLA03-09/pH 7.0; M: UFLA 03-10/pH 5.0; N: UFLA 03-10/pH 6.0; O: UFLA 03-10/pH7.0; P: UFLA 03-106/pH 5.0; Q: UFLA 03-106/pH 6.0; R: UFLA 03-106/pH7.0).
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Related In: Results  -  Collection

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f04: Identification and quantification of organic acids produced in liquidNBRIP culture medium at different initial pH values, inoculated withdifferent bacterial strains. Numbers in parentheses correspond to acidconcentration (mmol L−1). (Chromatograms - A: control/pH 5.0; B:control/pH 6.0; C: control/pH 7.0; D: UFLA 03-116/pH 5.0; E: UFLA 03-116/pH6.0; F: UFLA 03-116/pH 7.0; G: UFLA 03-08/pH 5.0; H: UFLA 03-08/pH 6.0; I:UFLA 03-08/pH 7.0; J: UFLA 03-09/pH 5.0 K: UFLA 03-09/pH 6.0; L: UFLA03-09/pH 7.0; M: UFLA 03-10/pH 5.0; N: UFLA 03-10/pH 6.0; O: UFLA 03-10/pH7.0; P: UFLA 03-106/pH 5.0; Q: UFLA 03-106/pH 6.0; R: UFLA 03-106/pH7.0).
Mentions: With respect to the identification, for the strain UFLA 03-08 (Rhizobiumtropici), the highest total acid concentration (malic acid, 18.90 mmolL−1) was found at an initial pH of 7.0; for this strain, acidproduction varied with the initial pH of the culture medium. Organic acidsidentified at pH 5.0 and 6.0 were respectively: 2-ketogluconic (1.28 mmolL−1) and lactic/succinic acid (0.37 mmol L−1). Other peakswere not identified in all pH values (Figure 4- Chromatograms G, H and I).

Bottom Line: The strains UFLA03-08, UFLA03-09, UFLA03-10 and UFLA03-106 solubilized Ca3(PO4)2 in liquid medium regardless of the initial pH, although without a significant difference between the treatments.The production of organic acids by these strains was assessed for all of the initial pH values investigated, and differences between the treatments were observed.There was no correlation between phosphorus solubilized from Ca3(PO4)2 in NBRIP liquid medium and the concentration of total organic acids at the different initial pH values.

View Article: PubMed Central - PubMed

Affiliation: Universidade Federal de Lavras, Programa de Pós-Graduação em Ciência do Solo, Departamento de Ciência do Solo, Universidade Federal de Lavras, Lavras, MG, Brasil, Programa de Pós-Graduação em Ciência do Solo, Departamento de Ciência do Solo, Universidade Federal de Lavras, Lavras, MG, Brazil.

ABSTRACT
The pH of the culture medium directly influences the growth of microorganisms and the chemical processes that they perform. The aim of this study was to assess the influence of the initial pH of the culture medium on the production of 11 low-molecular-weight organic acids and on the solubilization of calcium phosphate by bacteria in growth medium (NBRIP). The following strains isolated from cowpea nodules were studied: UFLA03-08 (Rhizobium tropici), UFLA03-09 (Acinetobacter sp.), UFLA03-10 (Paenibacillus kribbensis), UFLA03-106 (Paenibacillus kribbensis) and UFLA03-116 (Paenibacillus sp.). The strains UFLA03-08, UFLA03-09, UFLA03-10 and UFLA03-106 solubilized Ca3(PO4)2 in liquid medium regardless of the initial pH, although without a significant difference between the treatments. The production of organic acids by these strains was assessed for all of the initial pH values investigated, and differences between the treatments were observed. Strains UFLA03-09 and UFLA03-10 produced the same acids at different initial pH values in the culture medium. There was no correlation between phosphorus solubilized from Ca3(PO4)2 in NBRIP liquid medium and the concentration of total organic acids at the different initial pH values. Therefore, the initial pH of the culture medium influences the production of organic acids by the strains UFLA03-08, UFLA03-09, UFLA03-10 and UFLA03-106 but it does not affect calcium phosphate solubilization.

No MeSH data available.