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Initial pH of medium affects organic acids production but do not affect phosphate solubilization.

Marra LM, de Oliveira-Longatti SM, Soares CR, de Lima JM, Olivares FL, Moreira FM - Braz. J. Microbiol. (2015)

Bottom Line: The strains UFLA03-08, UFLA03-09, UFLA03-10 and UFLA03-106 solubilized Ca3(PO4)2 in liquid medium regardless of the initial pH, although without a significant difference between the treatments.The production of organic acids by these strains was assessed for all of the initial pH values investigated, and differences between the treatments were observed.There was no correlation between phosphorus solubilized from Ca3(PO4)2 in NBRIP liquid medium and the concentration of total organic acids at the different initial pH values.

View Article: PubMed Central - PubMed

Affiliation: Universidade Federal de Lavras, Programa de Pós-Graduação em Ciência do Solo, Departamento de Ciência do Solo, Universidade Federal de Lavras, Lavras, MG, Brasil, Programa de Pós-Graduação em Ciência do Solo, Departamento de Ciência do Solo, Universidade Federal de Lavras, Lavras, MG, Brazil.

ABSTRACT
The pH of the culture medium directly influences the growth of microorganisms and the chemical processes that they perform. The aim of this study was to assess the influence of the initial pH of the culture medium on the production of 11 low-molecular-weight organic acids and on the solubilization of calcium phosphate by bacteria in growth medium (NBRIP). The following strains isolated from cowpea nodules were studied: UFLA03-08 (Rhizobium tropici), UFLA03-09 (Acinetobacter sp.), UFLA03-10 (Paenibacillus kribbensis), UFLA03-106 (Paenibacillus kribbensis) and UFLA03-116 (Paenibacillus sp.). The strains UFLA03-08, UFLA03-09, UFLA03-10 and UFLA03-106 solubilized Ca3(PO4)2 in liquid medium regardless of the initial pH, although without a significant difference between the treatments. The production of organic acids by these strains was assessed for all of the initial pH values investigated, and differences between the treatments were observed. Strains UFLA03-09 and UFLA03-10 produced the same acids at different initial pH values in the culture medium. There was no correlation between phosphorus solubilized from Ca3(PO4)2 in NBRIP liquid medium and the concentration of total organic acids at the different initial pH values. Therefore, the initial pH of the culture medium influences the production of organic acids by the strains UFLA03-08, UFLA03-09, UFLA03-10 and UFLA03-106 but it does not affect calcium phosphate solubilization.

No MeSH data available.


Total organic acids (mmol L−1) and pH in liquid NBRIP mediumafter 10 days of incubation with bacterial strains in the presence ofCa3(PO4)2 for different initial valuesof pH (5.0, 6.0 and 7.0). Error bars represent the standard errors of themeans, n = 2.
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f03: Total organic acids (mmol L−1) and pH in liquid NBRIP mediumafter 10 days of incubation with bacterial strains in the presence ofCa3(PO4)2 for different initial valuesof pH (5.0, 6.0 and 7.0). Error bars represent the standard errors of themeans, n = 2.

Mentions: With respect to the quantification of organic acids, the strains UFLA 03-08, UFLA03-09, UFLA 03-10 and UFLA 03-106 produced organic acids in the culture media at allof the initial pH values studied (Figure 3).No organic acids were detected in the control without inoculation and in thenegative control, i.e., non-solubilizer strains UFLA 3-116.Considering all of the initial pH values, the highest total acid concentration wasproduced by the strain UFLA 03-106 (167.7 mmol L−1), with the lowerconcentrations being produced by UFLA 03-09 (104.4 mmol L−1), UFLA 03-10(65.35 mmol L−1), and UFLA 03-08 (21.52 mmol L−1). Consideringall of the strains, the highest total acid concentration was found at pH 7.0 (135.2mmol L−1), with lower concentrations being detected at pH 5.0 (113.65mmol L−1) and pH 6.0 (110.0 mmol L−1).


Initial pH of medium affects organic acids production but do not affect phosphate solubilization.

Marra LM, de Oliveira-Longatti SM, Soares CR, de Lima JM, Olivares FL, Moreira FM - Braz. J. Microbiol. (2015)

Total organic acids (mmol L−1) and pH in liquid NBRIP mediumafter 10 days of incubation with bacterial strains in the presence ofCa3(PO4)2 for different initial valuesof pH (5.0, 6.0 and 7.0). Error bars represent the standard errors of themeans, n = 2.
© Copyright Policy - open-access
Related In: Results  -  Collection

License
Show All Figures
getmorefigures.php?uid=PMC4507528&req=5

f03: Total organic acids (mmol L−1) and pH in liquid NBRIP mediumafter 10 days of incubation with bacterial strains in the presence ofCa3(PO4)2 for different initial valuesof pH (5.0, 6.0 and 7.0). Error bars represent the standard errors of themeans, n = 2.
Mentions: With respect to the quantification of organic acids, the strains UFLA 03-08, UFLA03-09, UFLA 03-10 and UFLA 03-106 produced organic acids in the culture media at allof the initial pH values studied (Figure 3).No organic acids were detected in the control without inoculation and in thenegative control, i.e., non-solubilizer strains UFLA 3-116.Considering all of the initial pH values, the highest total acid concentration wasproduced by the strain UFLA 03-106 (167.7 mmol L−1), with the lowerconcentrations being produced by UFLA 03-09 (104.4 mmol L−1), UFLA 03-10(65.35 mmol L−1), and UFLA 03-08 (21.52 mmol L−1). Consideringall of the strains, the highest total acid concentration was found at pH 7.0 (135.2mmol L−1), with lower concentrations being detected at pH 5.0 (113.65mmol L−1) and pH 6.0 (110.0 mmol L−1).

Bottom Line: The strains UFLA03-08, UFLA03-09, UFLA03-10 and UFLA03-106 solubilized Ca3(PO4)2 in liquid medium regardless of the initial pH, although without a significant difference between the treatments.The production of organic acids by these strains was assessed for all of the initial pH values investigated, and differences between the treatments were observed.There was no correlation between phosphorus solubilized from Ca3(PO4)2 in NBRIP liquid medium and the concentration of total organic acids at the different initial pH values.

View Article: PubMed Central - PubMed

Affiliation: Universidade Federal de Lavras, Programa de Pós-Graduação em Ciência do Solo, Departamento de Ciência do Solo, Universidade Federal de Lavras, Lavras, MG, Brasil, Programa de Pós-Graduação em Ciência do Solo, Departamento de Ciência do Solo, Universidade Federal de Lavras, Lavras, MG, Brazil.

ABSTRACT
The pH of the culture medium directly influences the growth of microorganisms and the chemical processes that they perform. The aim of this study was to assess the influence of the initial pH of the culture medium on the production of 11 low-molecular-weight organic acids and on the solubilization of calcium phosphate by bacteria in growth medium (NBRIP). The following strains isolated from cowpea nodules were studied: UFLA03-08 (Rhizobium tropici), UFLA03-09 (Acinetobacter sp.), UFLA03-10 (Paenibacillus kribbensis), UFLA03-106 (Paenibacillus kribbensis) and UFLA03-116 (Paenibacillus sp.). The strains UFLA03-08, UFLA03-09, UFLA03-10 and UFLA03-106 solubilized Ca3(PO4)2 in liquid medium regardless of the initial pH, although without a significant difference between the treatments. The production of organic acids by these strains was assessed for all of the initial pH values investigated, and differences between the treatments were observed. Strains UFLA03-09 and UFLA03-10 produced the same acids at different initial pH values in the culture medium. There was no correlation between phosphorus solubilized from Ca3(PO4)2 in NBRIP liquid medium and the concentration of total organic acids at the different initial pH values. Therefore, the initial pH of the culture medium influences the production of organic acids by the strains UFLA03-08, UFLA03-09, UFLA03-10 and UFLA03-106 but it does not affect calcium phosphate solubilization.

No MeSH data available.