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Identification of genes involved in breast cancer and breast cancer stem cells.

Apostolou P, Toloudi M, Papasotiriou I - Breast Cancer (Dove Med Press) (2015)

Bottom Line: Great progress has been made in its treatment but relapse is common.Finally, the gene expression levels of stemness transcription factors were measured.Knockdown experiments confirmed the impact of several of these on NANOG, OCT3/4, and SOX2 transcription factors.

View Article: PubMed Central - PubMed

Affiliation: Research and Development Department, Research Genetic Cancer Centre Ltd, Florina, Greece.

ABSTRACT
Breast cancer is the most frequent type of cancer in women. Great progress has been made in its treatment but relapse is common. One hypothesis to account for the high recurrence rates is the presence of cancer stem cells (CSCs), which have the ability to self-renew and differentiate into multiple malignant cell types. This study aimed to determine genes that are expressed in breast cancer and breast CSCs and to investigate their correlation with stemness. RNA was extracted from established breast cancer cell lines and from CSCs derived from five different breast cancer patients. DNA microarray analysis was performed and any upregulated genes were also studied in other cancer types, including colorectal and lung cancer. For genes that were expressed only in breast cancer, knockdown-based experiments were performed. Finally, the gene expression levels of stemness transcription factors were measured. The outcome of the analysis indicated a group of genes that were aberrantly expressed mainly in breast cancer cells with stemness properties. Knockdown experiments confirmed the impact of several of these on NANOG, OCT3/4, and SOX2 transcription factors. It seems that several genes that are not directly related with hormone metabolism and basic signal transduction pathways might have an important role in relapse and disease progression and, thus, can be targeted for new treatment approaches for breast cancer.

No MeSH data available.


Related in: MedlinePlus

Breast CSCs pre- and post-siRNA knockdown.Note: Representative images showing breast CSCs pre- and post-siRNA knockdown.Abbreviations: CSCs, cancer stem cells; siRNA, small interfering RNA; TMX2, thioredoxin-related transmembrane protein 2; FAM155B, family with sequence similarity 155, member B; PTGER3, prostaglandin E receptor 3 (subtype EP3); GPR3, G protein-coupled receptor 3; DDX49, DEAD (Asp-Glu-Ala-Asp) box polypeptide 49.
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f3-bctt-7-183: Breast CSCs pre- and post-siRNA knockdown.Note: Representative images showing breast CSCs pre- and post-siRNA knockdown.Abbreviations: CSCs, cancer stem cells; siRNA, small interfering RNA; TMX2, thioredoxin-related transmembrane protein 2; FAM155B, family with sequence similarity 155, member B; PTGER3, prostaglandin E receptor 3 (subtype EP3); GPR3, G protein-coupled receptor 3; DDX49, DEAD (Asp-Glu-Ala-Asp) box polypeptide 49.

Mentions: The knockdown experiments were satisfactory only for FAM155B, PTGER3, GPR3, TMX2, and DDX49. The reduction in expression ranged from 20% to 40% for FAM155B, 35%–40% for PTGER3, 35%–50% in GPR3, 10%–25% in TMX2, and around 40% for DDX49. The phenotype of the cells was not particularly affected, except in those in which TMX2 was knocked down (Figure 3). After TMX2 knockdown, the cells seem to obtain more rounded formation, compared with the control. However, the change is not too great, so be considered significant. The cell population also was not affected in all cells.


Identification of genes involved in breast cancer and breast cancer stem cells.

Apostolou P, Toloudi M, Papasotiriou I - Breast Cancer (Dove Med Press) (2015)

Breast CSCs pre- and post-siRNA knockdown.Note: Representative images showing breast CSCs pre- and post-siRNA knockdown.Abbreviations: CSCs, cancer stem cells; siRNA, small interfering RNA; TMX2, thioredoxin-related transmembrane protein 2; FAM155B, family with sequence similarity 155, member B; PTGER3, prostaglandin E receptor 3 (subtype EP3); GPR3, G protein-coupled receptor 3; DDX49, DEAD (Asp-Glu-Ala-Asp) box polypeptide 49.
© Copyright Policy
Related In: Results  -  Collection

License
Show All Figures
getmorefigures.php?uid=PMC4507490&req=5

f3-bctt-7-183: Breast CSCs pre- and post-siRNA knockdown.Note: Representative images showing breast CSCs pre- and post-siRNA knockdown.Abbreviations: CSCs, cancer stem cells; siRNA, small interfering RNA; TMX2, thioredoxin-related transmembrane protein 2; FAM155B, family with sequence similarity 155, member B; PTGER3, prostaglandin E receptor 3 (subtype EP3); GPR3, G protein-coupled receptor 3; DDX49, DEAD (Asp-Glu-Ala-Asp) box polypeptide 49.
Mentions: The knockdown experiments were satisfactory only for FAM155B, PTGER3, GPR3, TMX2, and DDX49. The reduction in expression ranged from 20% to 40% for FAM155B, 35%–40% for PTGER3, 35%–50% in GPR3, 10%–25% in TMX2, and around 40% for DDX49. The phenotype of the cells was not particularly affected, except in those in which TMX2 was knocked down (Figure 3). After TMX2 knockdown, the cells seem to obtain more rounded formation, compared with the control. However, the change is not too great, so be considered significant. The cell population also was not affected in all cells.

Bottom Line: Great progress has been made in its treatment but relapse is common.Finally, the gene expression levels of stemness transcription factors were measured.Knockdown experiments confirmed the impact of several of these on NANOG, OCT3/4, and SOX2 transcription factors.

View Article: PubMed Central - PubMed

Affiliation: Research and Development Department, Research Genetic Cancer Centre Ltd, Florina, Greece.

ABSTRACT
Breast cancer is the most frequent type of cancer in women. Great progress has been made in its treatment but relapse is common. One hypothesis to account for the high recurrence rates is the presence of cancer stem cells (CSCs), which have the ability to self-renew and differentiate into multiple malignant cell types. This study aimed to determine genes that are expressed in breast cancer and breast CSCs and to investigate their correlation with stemness. RNA was extracted from established breast cancer cell lines and from CSCs derived from five different breast cancer patients. DNA microarray analysis was performed and any upregulated genes were also studied in other cancer types, including colorectal and lung cancer. For genes that were expressed only in breast cancer, knockdown-based experiments were performed. Finally, the gene expression levels of stemness transcription factors were measured. The outcome of the analysis indicated a group of genes that were aberrantly expressed mainly in breast cancer cells with stemness properties. Knockdown experiments confirmed the impact of several of these on NANOG, OCT3/4, and SOX2 transcription factors. It seems that several genes that are not directly related with hormone metabolism and basic signal transduction pathways might have an important role in relapse and disease progression and, thus, can be targeted for new treatment approaches for breast cancer.

No MeSH data available.


Related in: MedlinePlus